A noninvasive sampling method for genetic analysis of the West Indian manatee (<i>Trichechus manatus</i>)

Muschett, Giselle; Bonacic, Cristián; Vianna, Juliana A.

doi:10.1111/j.1748-7692.2009.00310.x

Cited by 7 publications

(9 citation statements)

References 28 publications

(41 reference statements)

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“…Muschett et al. (2009) proposed the collection of faeces of the West Indian manatee as a source of DNA, which proved to be a completely non‐invasive method of sampling.…”

Section: Resultsmentioning

confidence: 99%

“…In this section, we review six important contributions to invasive and non-invasive sampling methods, as used in South American aquatic mammals: fur seal skin samples (Gemmell & Majluf 1997), skin biopsies from southern right whales (Oliveira et al 2009b), faeces from the West Indian manatee (Muschett et al 2009) and from the South American marine otter (Valqui et al 2010), DNA extracted from formalin fixed specimens (Rodriguez et al 2002) and skin biopsies from Tursiops spp. (Krützen et al 2002).…”

Section: Invasive and Non-invasive Sampling Methods For Genetic Analysismentioning

confidence: 99%

“…The calculated sex ratio was two females: one male, which is similar to most species of baleen whales. Muschett et al (2009) proposed the collection of faeces of the West Indian manatee as a source of DNA, which proved to be a completely non-invasive method of sampling. Faecal samples yielded enough high-quality DNA for satisfactory amplification (Tringali et al 2008).…”

Section: Invasive and Non-invasive Sampling Methods For Genetic Analysismentioning

confidence: 99%

See 2 more Smart Citations

Conservation genetics of South American aquatic mammals: an overview of gene diversity, population structure, phylogeography, non‐invasive methods and forensics

Oliveira¹,

Castro²,

Cárdenas‐Alayza³

et al. 2011

Mammal Review

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Most aquatic mammals have high dispersal potential, and there are often severe conservation concerns related to their legal or illegal harvesting. Therefore, economic, social and forensic factors often arise in decisions relating to their population management. Molecular markers are essential tools in modern conservation genetics, revealing previously unknown aspects of aquatic mammal behaviour, natural history, population structure and demography. Molecular markers also have been used to define management units, to recognize taxonomic units, to conduct forensic analyses and to control illegal wildlife trade, providing valuable information for decision‐making in wildlife conservation and management. We review studies published in peer‐reviewed journals between 1993 and 2010, in which genetic approaches have been applied to conservation‐related issues involving natural populations of 25 species of aquatic mammals in South America. These studies cover just 34% of the 70 aquatic mammal species recorded in South America. Most of the studies are related to population structure, phylogeography, gene flow and dispersal movements. In addition, recent findings relate to evolutionarily significant units, management units, forensics and conservation policy. Finally, we look to the future and, based on numbers of studies and conservation concerns, suggest which species, geographic areas and genetic studies should be prioritized. Moreover, we discuss constraints on research and suggest collaborative works that would provide critical information towards the effective conservation and management of aquatic mammals in South America.

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“…Muschett et al. (2009) proposed the collection of faeces of the West Indian manatee as a source of DNA, which proved to be a completely non‐invasive method of sampling.…”

Section: Resultsmentioning

confidence: 99%

Section: Invasive and Non-invasive Sampling Methods For Genetic Analysismentioning

confidence: 99%

Section: Invasive and Non-invasive Sampling Methods For Genetic Analysismentioning

confidence: 99%

See 1 more Smart Citation

Conservation genetics of South American aquatic mammals: an overview of gene diversity, population structure, phylogeography, non‐invasive methods and forensics

Oliveira¹,

Castro²,

Cárdenas‐Alayza³

et al. 2011

Mammal Review

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show abstract

“…PCR amplification conditions were: 94°C for 2 min, 34 cycles of 94°C for 30 s, 55°C for 45 s and 72°C for 40 s, with a final extension period of 72°C for 10 min. Low quality samples (faeces and bones) were amplified using LTMCR01 and HDDCR01 primer pair and previously published PCR conditions [27]. Volume reaction mixture contained 10µl Maxima® HotStart PCR Master Mix (Fermentas), and 0.5 µM of each primer.…”

Section: Methodsmentioning

confidence: 99%

Phylogeography and Sex-Biased Dispersal across Riverine Manatee Populations (Trichechus inunguis and Trichechus manatus) in South America

et al. 2012

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Phylogeographic patterns and sex-biased dispersal were studied in riverine populations of West Indian (Trichechus manatus) and Amazonian manatees (T. inunguis) in South America, using 410bp D-loop (Control Region, Mitochondrial DNA) sequences and 15 nuclear microsatellite loci. This multi-locus approach was key to disentangle complex patterns of gene flow among populations. D-loop analyses revealed population structuring among all Colombian rivers for T. manatus, while microsatellite data suggested no structure. Two main populations of T. inunguis separating the Colombian and Peruvian Amazon were supported by analysis of the D-loop and microsatellite data. Overall, we provide molecular evidence for differences in dispersal patterns between sexes, demonstrating male-biased gene flow dispersal in riverine manatees. These results are in contrast with previously reported levels of population structure shown by microsatellite data in marine manatee populations, revealing low habitat restrictions to gene flow in riverine habitats, and more significant dispersal limitations for males in marine environments.

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“…PCR amplification conditions were: 94uC for 2 min, 34 cycles of 94uC for 30 s, 55uC for 45 s and 72uC for 40 s, with a final extension period of 72uC for 10 min. Low quality samples (faeces and bones) were amplified using LTMCR01 and HDDCR01 primer pair and previously published PCR conditions [27]. Volume reaction mixture contained 10ml MaximaH HotStart PCR Master Mix (Fermentas), and 0.5 mM of each primer.…”

Section: Pcr Analysis and Sequencingmentioning

confidence: 99%

Phylogeography and Sex-Biased Dispersal across Riverine Manatee Populations (Trichechus inunguis and Trichechus manatus) in South America

Satizábal¹,

Mignucci‐Giannoni²,

Duchêne³

et al. 2019

Preprint

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A noninvasive sampling method for genetic analysis of the West Indian manatee (Trichechus manatus)

Cited by 7 publications

References 28 publications

Conservation genetics of South American aquatic mammals: an overview of gene diversity, population structure, phylogeography, non‐invasive methods and forensics

Conservation genetics of South American aquatic mammals: an overview of gene diversity, population structure, phylogeography, non‐invasive methods and forensics

Phylogeography and Sex-Biased Dispersal across Riverine Manatee Populations (Trichechus inunguis and Trichechus manatus) in South America

Phylogeography and Sex-Biased Dispersal across Riverine Manatee Populations (Trichechus inunguis and Trichechus manatus) in South America

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