A Nontoxic Chimeric Cholera Toxin Analog<sup>a</sup>

Boesman-Finkelstein, Mary; Peterson, Johnny W.; Thai, L. S.; Finkelstein, R. A.

doi:10.1111/j.1749-6632.1996.tb52973.x

Cited by 3 publications

(2 citation statements)

References 3 publications

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“…The CT-2* protein, a mutant form of the cholera toxin, produced by a vaccine strain of V. cholerae, was purified to homogeneity by sodium hexametaphosphate precipitation, affinity purification on a galactose column and Sephadex G75 gel filtration chromatography as described earlier [25][26][27][28]. The purified toxin was dissolved in pyrogen-free water, and the lipopolysaccharide (LPS) contamination was determined by the Limulus amebocyte lysate assay (QCL-1000kit, BioWhitaker, Walkerville, MD).…”

Section: Mutant Cholera Toxin (Ct-2*)mentioning

confidence: 99%

“…The purified toxin was dissolved in pyrogen-free water, and the lipopolysaccharide (LPS) contamination was determined by the Limulus amebocyte lysate assay (QCL-1000kit, BioWhitaker, Walkerville, MD). The amount of LPS detected in 1 μg of CT-2* (amount used as adjuvant in mice) was 0.5 pg, which did not stimulate production of any cytokine in the mouse ligated ileal loops [25][26][27][28].…”

Section: Mutant Cholera Toxin (Ct-2*)mentioning

confidence: 99%

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Intranasal immunization with synthetic peptides corresponding to the E6 and E7 oncoproteins of human papillomavirus type 16 induces systemic and mucosal cellular immune responses and tumor protection

Manuri

Nehete

Reisenauer

et al. 2007

Vaccine

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The E6 and E7 oncoproteins of the high-risk HPV type16 represent ideal targets for HPV vaccine development, they being consistently expressed in cervical cancer lesions. Since HPV-16 is primarily transmitted through genital mucosal route, mucosal immune responses constitute an essential feature for vaccination strategies against HPV-associated lesions. We present here evidence showing that mucosal immunization of mice by the intranasal route with a mixture of peptides E7 44-62 and E6 43-57 from the E7 and E6 oncoproteins of HPV-16, respectively, using a mutant cholera toxin adjuvant (CT-2*), primed strong antigen-specific cellular immune responses in systemic and mucosal tissues. Significant levels of IFN-γ production by both CD4 and CD8 cells were observed along with CTL responses that were effective against both peptide-pulsed targets as well as syngeneic tumor cells (TC-1) expressing the cognate E6 and E7 proteins. Furthermore, mice immunized with the peptide mixture and CT-2* effectively resisted TC-1 tumor challenge. These results together with our earlier observations that T cell responses to these peptides correlate with recurrence-free survival in women after ablative treatment for HPV-associated cervical intraepithelial neoplasia, support the potential of these E6 and E7 peptides for inclusion in vaccine formulations.

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Section: Mutant Cholera Toxin (Ct-2*)mentioning

confidence: 99%

Section: Mutant Cholera Toxin (Ct-2*)mentioning

confidence: 99%

Intranasal immunization with synthetic peptides corresponding to the E6 and E7 oncoproteins of human papillomavirus type 16 induces systemic and mucosal cellular immune responses and tumor protection

Manuri

Nehete

Reisenauer

et al. 2007

Vaccine

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Alpha and beta myosin isoforms and human atrial and ventricular contraction

Walklate

Ferrantini

Johnson

et al. 2021

Cell. Mol. Life Sci.

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Human atrial and ventricular contractions have distinct mechanical characteristics including speed of contraction, volume of blood delivered and the range of pressure generated. Notably, the ventricle expresses predominantly β-cardiac myosin while the atrium expresses mostly the α-isoform. In recent years exploration of the properties of pure α- & β-myosin isoforms have been possible in solution, in isolated myocytes and myofibrils. This allows us to consider the extent to which the atrial vs ventricular mechanical characteristics are defined by the myosin isoform expressed, and how the isoform properties are matched to their physiological roles. To do this we Outline the essential feature of atrial and ventricular contraction; Explore the molecular structural and functional characteristics of the two myosin isoforms; Describe the contractile behaviour of myocytes and myofibrils expressing a single myosin isoform; Finally we outline the outstanding problems in defining the differences between the atria and ventricles. This allowed us consider what features of contraction can and cannot be ascribed to the myosin isoforms present in the atria and ventricles.

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A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens

Lomada

Gambhira

Nehete

et al. 2004

Vaccine

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A Nontoxic Chimeric Cholera Toxin Analog^a

Cited by 3 publications

References 3 publications

Intranasal immunization with synthetic peptides corresponding to the E6 and E7 oncoproteins of human papillomavirus type 16 induces systemic and mucosal cellular immune responses and tumor protection

Intranasal immunization with synthetic peptides corresponding to the E6 and E7 oncoproteins of human papillomavirus type 16 induces systemic and mucosal cellular immune responses and tumor protection

Alpha and beta myosin isoforms and human atrial and ventricular contraction

A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens

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A Nontoxic Chimeric Cholera Toxin Analoga

Cited by 3 publications

References 3 publications

Intranasal immunization with synthetic peptides corresponding to the E6 and E7 oncoproteins of human papillomavirus type 16 induces systemic and mucosal cellular immune responses and tumor protection

Intranasal immunization with synthetic peptides corresponding to the E6 and E7 oncoproteins of human papillomavirus type 16 induces systemic and mucosal cellular immune responses and tumor protection

Alpha and beta myosin isoforms and human atrial and ventricular contraction

A two-codon mutant of cholera toxin lacking ADP-ribosylating activity functions as an effective adjuvant for eliciting mucosal and systemic cellular immune responses to peptide antigens

Contact Info

Product

Resources

About

A Nontoxic Chimeric Cholera Toxin Analog^a