A nonviral pHEMA+chitosan nanosphere-mediated high-efficiency gene delivery system

Eroğlu, Erdal; Tiwari, Pooja; Waffo, Alain Bopda; Miller, Michael E.; Vig, Komal; Dennis, Vida A.; Singh, Shree R.

doi:10.2147/ijn.s43168

Cited by 15 publications

(8 citation statements)

References 37 publications

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“…The GFP gene was amplified by PCR using previously published GFP plasmid vector [ 26 ] as a template, forward and reverse primers ( Table 1 ) with Bam HI and Not I restriction sites, respectively. The amplified GFP gene was inserted into the phCMV1 vector following the same protocol and conditions as described above.…”

Section: Methodsmentioning

confidence: 99%

Immunogenicity of RSV F DNA Vaccine in BALB/c Mice

Eroğlu

Singh

Bawage

et al. 2016

Advances in Virology

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Respiratory syncytial virus (RSV) causes severe acute lower respiratory tract disease leading to numerous hospitalizations and deaths among the infant and elderly populations worldwide. There is no vaccine or a less effective drug available against RSV infections. Natural RSV infection stimulates the Th1 immune response and activates the production of neutralizing antibodies, while earlier vaccine trials that used UV-inactivated RSV exacerbated the disease due to the activation of the allergic Th2 response. With a focus on Th1 immunity, we developed a DNA vaccine containing the native RSV fusion (RSV F) protein and studied its immune response in BALB/c mice. High levels of RSV specific antibodies were induced during subsequent immunizations. The serum antibodies were able to neutralize RSV in vitro. The RSV inhibition by sera was also shown by immunofluorescence analyses. Antibody response of the RSV F DNA vaccine showed a strong Th1 response. Also, sera from RSV F immunized and RSV infected mice reduced the RSV infection by 50% and 80%, respectively. Our data evidently showed that the RSV F DNA vaccine activated the Th1 biased immune response and led to the production of neutralizing antibodies, which is the desired immune response required for protection from RSV infections.

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Section: Methodsmentioning

confidence: 99%

Immunogenicity of RSV F DNA Vaccine in BALB/c Mice

Eroğlu

Singh

Bawage

et al. 2016

Advances in Virology

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“…Both the nanocomposite formulations were characterized using zeta potential and fourier transform infrared spectroscopy (FT-IR) analysis [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

Novel pegylated silver coated carbon nanotubes kill Salmonella but they are non-toxic to eukaryotic cells

et al. 2015

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BackgroundResistance of food borne pathogens such as Salmonella to existing antibiotics is of grave concern. Silver coated single walled carbon nanotubes (SWCNTs-Ag) have broad-spectrum antibacterial activity and may be a good treatment alternative. However, toxicity to human cells due to their physico-chemical properties is a serious public health concern. Although pegylation is commonly used to reduce metal nanoparticle toxicity, SWCNTs-Ag have not been pegylated as yet, and the effect of pegylation of SWCNTs-Ag on their anti-bacterial activity and cell cytotoxicity remains to be studied. Further, there are no molecular studies on the anti-bacterial mechanism of SWCNTs-Ag or their functionalized nanocomposites.Materials and methodsIn this study we created novel pegylated SWCNTS-Ag (pSWCNTs-Ag), and employed 3 eukaryotic cell lines to evaluate their cytotoxicity as compared to plain SWCNTS-Ag. Simultaneously, we evaluated their antibacterial activity on Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) by the MIC and growth curve assays. In order to understand the possible mechanisms of action of both SWCNTs-Ag and pSWCNTs-Ag, we used electron microscopy (EM) and molecular studies (qRT-PCR).ResultspSWCNTs-Ag inhibited Salmonella Typhimurium at 62.5 μg/mL, while remaining non-toxic to human cells. By comparison, plain SWCNTs-Ag were toxic to human cells at 62.5 μg/mL. EM analysis revealed that bacteria internalized either of these nanocomposites after the outer cell membranes were damaged, resulting in cell lysis or expulsion of cytoplasmic contents, leaving empty ghosts. The expression of genes regulating the membrane associated metabolic transporter system (artP, dppA, and livJ), amino acid biosynthesis (trp and argC) and outer membrane integrity (ompF) protiens, was significantly down regulated in Salmonella treated with both pSWCNTs-Ag and SWCNTs-Ag. Although EM analysis of bacteria treated with either SWCNTs-Ag or pSWCNTs-Ag revealed relatively similar morphological changes, the expression of genes regulating the normal physiological processes of bacteria (ybeF), quorum sensing (sdiA), outer membrane structure (safC), invasion (ychP) and virulence (safC, ychP, sseA and sseG) were exclusively down regulated several fold in pSWCNTs-Ag treated bacteria.ConclusionsAltogether, the present data shows that our novel pSWCNTs-Ag are non-toxic to human cells at their bactericidal concentration, as compared to plain SWCNTS-Ag. Therefore, pSWCNTs-Ag may be safe alternative antimicrobials to treat foodborne pathogens.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-015-0085-5) contains supplementary material, which is available to authorized users.

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“…Nanomaterials have been investigated in the medical field for drugs (Cao et al, 2014; Ranganathan et al, 2012; Tan et al, 2011; Zhao et al, 2013) and genes carriers (Banizs et al, 2014; Eroglu et al, 2013; Liu et al, 2014), cancer therapy (Frank et al., 2014; Jabir et al., 2012; Marques et al, 2014; Wang et al, 2013), gene therapy (Rodriguez-Gascon et al, 2014), antibacterial agents (Amrol, 2007; Seil and Webster, 2012; Taylor and Webster, 2011), antiviral agents (du Toit et al, 2010; Mahajan et al, 2012; Parboosing et al, 2012), tissue engineering (Mazaheri et al., 2015; Partha and Conyers, 2009; Tautzenberger et al., 2012; Tonelli et al, 2012), medical diagnosis (Chen et al, 2013; Kang et al, 2015; Madani et al, 2013), medical imaging (Cao et al, 2014; Peng et al, 2008; Sheng et al, 2014; Yi et al, 2014), etc., which bring many benefits to humans. At the same time, studies on the biosafety of nanomaterials have been soaring in recent years (Vega-Villa et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Potential applications and human biosafety of nanomaterials used in nanomedicine

Bowman

et al. 2017

J of Applied Toxicology

138

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With the rapid development of nanotechnology, potential applications of nanomaterials in medicine have been widely researched in recent years. Nanomaterials themselves can be used as image agents or therapeutic drugs, and for drug and gene delivery, biological devices, nanoelectronic biosensors or molecular nanotechnology. As the composition, morphology, chemical properties, implant sites as well as potential applications become more and more complex, human biosafety of nanomaterials for clinical use has become a major concern. If nanoparticles accumulate in the human body or interact with the body molecules or chemical components, health risks may also occur. Accordingly, the unique chemical and physical properties, potential applications in medical fields, as well as human biosafety in clinical trials are reviewed in this study. Finally, this article tries to give some suggestions for future work in nanomedicine research.

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A nonviral pHEMA+chitosan nanosphere-mediated high-efficiency gene delivery system

Cited by 15 publications

References 37 publications

Immunogenicity of RSV F DNA Vaccine in BALB/c Mice

Immunogenicity of RSV F DNA Vaccine in BALB/c Mice

Novel pegylated silver coated carbon nanotubes kill Salmonella but they are non-toxic to eukaryotic cells

Potential applications and human biosafety of nanomaterials used in nanomedicine

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