A NOS1 Gene Polymorphism Associated with Asthma and Specific Immunoglobulin E Response to Mite Allergens in a Colombian Population

Martínez, Beatriz; Barrios, Katherine; Vergara, Candelaria; Mercado, Dilia; Jiménez, Silvia; Gusmão, Leonor; Caraballo, Luis

doi:10.1159/000103221

Cited by 23 publications

(27 citation statements)

References 80 publications

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“…In the past, measurement of small nuclear polymorphisms (SNPs) and quantitative mRNA expression by PCR technologies have been well established which can easily differentiate between highly homologous isoforms [26][27][28][29][30][31][32][33][34].…”

Section: Methods Developmentmentioning

confidence: 99%

Current affairs in quantitative targeted proteomics: multiple reaction monitoring-mass spectrometry

Yocum¹,

Chinnaiyan²

2009

Briefings in Functional Genomics and Proteomics

109

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Quantitative targeted proteomics has recently taken front stage in the proteomics community. Centered on multiple reaction monitoring^mass spectrometry (MRM^MS) methodologies, quantitative targeted proteomics is being used in the verification of global proteomics data, the discovery of lower abundance proteins, protein post-translational modifications, discrimination of select highly homologous protein isoforms and as the final step in biomarker discovery. An older methodology utilized with small molecule analysis, the proteomics community is making great technological strides to develop MRM^MS as the next method to address previously challenging issues in global proteomics experimentation, namely dynamic range, identification of post-translational modifications, sensitivity and selectivity of measurement which will undoubtedly further biomedical knowledge.This brief review will provide a general introduction of MRM^MS and highlight its novel application for targeted quantitative proteomic experimentations.

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Section: Methods Developmentmentioning

confidence: 99%

Current affairs in quantitative targeted proteomics: multiple reaction monitoring-mass spectrometry

Yocum¹,

Chinnaiyan²

2009

Briefings in Functional Genomics and Proteomics

109

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“…Four hundred and seventeen patients with asthma were recruited from the Social Security Clinic and public outpatient health centers between the years 2002 and 2005 in Cartagena, a tropical city on the Caribbean Coast of Colombia. Asthma was defined according to the Global Initiative for Asthma criteria, using a standardized questionnaire previously tested [26,27,28] in patients with a history of physician-diagnosed asthma. The diagnosis was confirmed by a physician belonging to the research staff, supported by a clear clinical history with clinical symptoms.…”

Section: Methodsmentioning

confidence: 99%

“…Since Blomia tropicalis and Dermatophagoides pteronyssinus are the main source of sensitization in tropical environments [33,34,35], and the prevalence of IgE sensitization to other aeroallergens is very low in asthmatics and in the general population of Cartagena [36], specific IgE against these 2 mite extracts was determined by indirect ELISA in all individuals, as described previously [28]. Absorbance was measured at 405 nm using a Spectrophotometer (Spectra Max 250, Molecular Devices, Sunnyvale, Calif., USA) and expressed as optical density (OD) units.…”

Section: Methodsmentioning

confidence: 99%

The C-509T Promoter Polymorphism of the Transforming Growth Factor Beta-1 Gene Is Associated with Levels of Total and Specific IgE in a Colombian Population

Acevedo

Vergara

Gusmão

et al. 2009

Int Arch Allergy Immunol

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Background: The C-509T polymorphism of the transforming growth factor beta-1 (TGFB1) gene has been associated with asthma and asthma-related phenotypes, but its influence on total and specific IgE levels is controversial. Objective: To investigate the association between C-509T and asthma, as well as total IgE and specific IgE against Blomia tropicalis and Dermatophagoides pteronyssinus, in a Colombian population. Methods: The study population consisted of 417 asthmatics, 390 controls and 116 nuclear families. The C-509T polymorphism was genotyped using single-base extension minisequencing or Taq Man probes. IgE and TGFβ1 levels were measured by ELISA. Regression analysis and family-based association tests were performed in cases/controls and families. Associations were corrected by population structure using a panel of 52 ancestry informative markers in the case-control dataset. Results: There was no association between C-509T and asthma. In asthmatics, the CC genotype was associated with higher total IgE levels compared with the other genotypes [mean IgE: 2.81 ± 0.42 vs. 2.71 ± 0.45 log IU/ml; p = 0.016, corrected p value (p_c) = 0.019]. When only atopic asthmatics were included, the significance remained (p = 0.02, p_c = 0.03). In the family-based analyses, the C allele was associated with higher total IgE levels (p = 0.02) and the CC genotype with specific IgE to D. pteronyssinus (p = 0.01). Conclusions: C-509T is associated with total IgE levels and specific IgE to D. pteronyssinus in asthmatic patients. In contrast to other studies, we found the CC genotype to be associated with higher levels of total and specific IgE. Differences in the frequency of this allele among populations could alter its effects as a risk factor for asthma-associated phenotypes.

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“…Blood samples were collected from all subjects for serum measurements of total serum IgE (tIgE) concentrations and DNA extraction for genotyping. Asthma definition and tIgE measurements were previously described (23)(24)(25)(26) for each population as explained in detail in the online supplement.…”

Section: Subjectsmentioning

confidence: 99%

Gene Encoding Duffy Antigen/Receptor for Chemokines Is Associated with Asthma and IgE in Three Populations

Vergara¹,

Tsai²,

Grant³

et al. 2008

Am J Respir Crit Care Med

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Rationale: Asthma prevalence and severity are high among underserved minorities, including those of African descent. The Duffy antigen/receptor for chemokines is the receptor for Plasmodium vivax on erythrocytes and functions as a chemokine-clearing receptor. Unlike European populations, decreased expression of the receptor on erythrocytes is common among populations of African descent, and results from a functional T-46C polymorphism (rs2814778) in the promoter. This variant provides an evolutionary advantage in malaria-endemic regions, because Duffy antigen/receptor for chemokines-negative erythrocytes are more resistant to infection by P. vivax. Objectives: To determine the role of the rs2814778 polymorphism in asthma and atopy as measured by total serum IgE levels among four populations of African descent (African Caribbean, African American, Brazilian, and Colombian) and a European American population. Methods: Family-based association tests were performed in each of the five populations to test for association between the rs2814778 polymorphism and asthma or total IgE concentration. Measurements and Main Results: Asthma was significantly associated with the rs2814778 polymorphism in the African Caribbean, Colombian, and Brazilian families (P , 0.05). High total IgE levels were associated with this variant in African Caribbean and Colombian families (P , 0.05). The variant allele was not polymorphic among European Americans. Conclusions: Susceptibility to asthma and atopy among certain populations of African descent is influenced by a functional polymorphism in the gene encoding Duffy antigen/receptor for chemokines. This genetic variant, which confers resistance to malarial parasitic infection, may also partially explain ethnic differences in morbidity of asthma.

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A NOS1 Gene Polymorphism Associated with Asthma and Specific Immunoglobulin E Response to Mite Allergens in a Colombian Population

Cited by 23 publications

References 80 publications

Current affairs in quantitative targeted proteomics: multiple reaction monitoring-mass spectrometry

Current affairs in quantitative targeted proteomics: multiple reaction monitoring-mass spectrometry

The C-509T Promoter Polymorphism of the Transforming Growth Factor Beta-1 Gene Is Associated with Levels of Total and Specific IgE in a Colombian Population

Gene Encoding Duffy Antigen/Receptor for Chemokines Is Associated with Asthma and IgE in Three Populations

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