A “notch” in the cellular communication network in response to anoxia by wood frog (Rana sylvatica)

Gupta, Aakriti; Storey, Kenneth B.

doi:10.1016/j.cellsig.2022.110305

Cited by 2 publications

(1 citation statement)

References 54 publications

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“…Thus, suppression of DNMT3B-S during recovery from rehydration may promote transcription of survival-associated processes, rather than dysregulated proliferation. Some signal transduction pathways that DNMT3B is known to influence have previously been shown to be involved in regulating wood frog stress models, including NF-κB, STAT3, PI3K-Akt, and Notch pathways (Douglas et al, 2022;Gupta & Storey, 2022;So et al, 2020;Zhang & Storey, 2014). However, research also suggests an accessory role of some DNMT3B forms, the suppression of which is expected to achieve a similar purpose (Duymich et al, 2016).…”

Section: Mbd2 and Dnmt3b-smentioning

confidence: 99%

Response of DNA and Histone Lysine Methylation Regulators During Anoxia-Reoxygenation and Dehydration-Rehydration in Wood Frog (Rana sylvatica) Skeletal Muscle

Douglas

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The ability of wood frogs, Rana sylvatica¸ to survive freezing and clinical death during harsh northern winters necessitates well-grounded mechanisms for managing extreme anoxia, dehydration, and reperfusion damage. These stresses are major instigators of pathogenesis yet to be overcome by humans. Contemporary efforts in the field are focused on interrogating stress-specific mediators that play a cytoprotective role in vital tissues such as skeletal muscle, providing valuable information to the biomedical community. Herein, the potential role of DNA and histone lysine methylation enzymes are examined in wood frog skeletal muscle in response to 24 h anoxia and 40% dehydration independently, and recovery from both stresses. This thesis demonstrates a condition-specific response of many epigenetic methylation regulators, highlighting some conserved similarities in comparison to prior freeze-thaw models. These findings support an integral role of epigenetic regulators in survival of hypometabolic stresses, most prominently during recovery stages. These cytoprotective effects are likely attributed to functional roles in transcriptional suppression during hypometabolism and activation during recovery, but also alternative roles based on known interactions with regulators of the cell cycle and repair pathways.iii ACKNOWLEDGEMENTSTo everyone at the Storey Lab, and to the extended fam at Carleton, thank you so much for all the great memories over the years! Every single one of you has been a joy to meet, and I will forever cherish the time we spent together grinding away in the office, at the bench, and furiously 'grading' at Ollie's. Ken and Jan, you took a chance on a rusty headbanger that had little prior lab experience and provided me with a hopeful new direction in life by accepting me into your lab. It is inspiring the number of lives you have positively impacted with your wisdom and guidance, as is this welcoming and diverse space for research you have created, which has felt like a safehouse from the storms of life. You have given me the means to nurture my scientific abilities, and facilitated my journey to being a productive member of society that is proud of the work I do. You two are truly legendary humans in so many ways. I'd also like to give a special thanks to my mentors Sam and Tighe. Your dedication and enthusiasm for helping others, and for this work, have been contagious. You have awoken a beast-of-research in me, while taming my technical and analytic skills into something of finesse that I am confident in. You have shown me how to have a critical eye for research, and what it takes to be a good scientist in this world, but also how fun and rewarding it can be. I will always hold your impromptu lessons close to me and strive to be more like you in my work. To the lads (Mackenzie, Kupa, Wale, Saif, and Gurjit), and especially the 'lunch ladies' (Aakriti, Aline, Anchal, Maire, Rasha, and Sarah), I would not be where I am today without you. Your kindness and guidance have been monumental to my success in...

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Section: Mbd2 and Dnmt3b-smentioning

confidence: 99%

Response of DNA and Histone Lysine Methylation Regulators During Anoxia-Reoxygenation and Dehydration-Rehydration in Wood Frog (Rana sylvatica) Skeletal Muscle

Douglas

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Activation of p53 in anoxic freshwater crayfish, Faxonius virilis

Gupta

Breedon

Storey

2022

Journal of Experimental Biology

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Tumor suppressing transcription factor p53 regulates multiple pathways including DNA repair, cell survival, apoptosis, and autophagy. The current work studies stress-induced activation of p53 in anoxic crayfish (Faxonius virilis). Relative levels of target proteins and mRNAs involved in the DNA damage response was measured in normoxic control and anoxic hepatopancreas and tail muscle. Phosphorylation levels of p53 was assessed using immunoblotting at sites known to be phosphorylated (Serine 15 and 37) in response to DNA damage or reduced oxygen signaling. The capacity for DNA binding by phospho-p53 was also measured, followed by transcript analysis of a potentially pro-apoptotic downstream target, the etoposide induced (ei24) gene. Following this, both inhibitor (MDM2) and activator (p19-ARF) protein levels in response to low oxygen stress were studied. The results showed an increase in p53 levels during anoxia in both hepatopancreases and tail muscle. Increased transcript levels of ei24, a downstream target of p53, support the activation of p53 under anoxic stress. Cytoplasmic accumulation of Ser-15 p-p53 was observed during anoxia when proteins from cytoplasmic and nuclear fractions were measured. Increased cytoplasmic concentration is known to initiate an apoptotic response, which can be assumed as a preparatory step to prevent autophagy. The results suggest that p53 might play a protective role in crayfish defense against low oxygen stress. Understanding how anoxia-tolerant organisms are able to protect against DNA damage could provide important clues towards survival under metabolic rate depression and preparation for recovery to minimize damage.

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A “notch” in the cellular communication network in response to anoxia by wood frog (Rana sylvatica)

Cited by 2 publications

References 54 publications

Response of DNA and Histone Lysine Methylation Regulators During Anoxia-Reoxygenation and Dehydration-Rehydration in Wood Frog (Rana sylvatica) Skeletal Muscle

Response of DNA and Histone Lysine Methylation Regulators During Anoxia-Reoxygenation and Dehydration-Rehydration in Wood Frog (Rana sylvatica) Skeletal Muscle

Activation of p53 in anoxic freshwater crayfish, Faxonius virilis

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