A Note on the Effect of Different Types of Agar on the Recovery of Heated Escherichia coli K‐12

Pellón, J R; Gómez, Reinaldo F.

doi:10.1111/j.1365-2672.1979.tb00828.x

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…Lower recoveries in agar media than in liquid have been attributed to impurities in the agar (Jacobs and Harris 1961;Pellon and Gomez 1979), cell density effects (Payne 1978) or limited diffusion of nutrients to cells and of toxic products away from cells (Miller and Davey 1965). In our case, it is possible that a toxic product, e.g.…”

Section: Resultsmentioning

confidence: 86%

A comparison of solid and liquid media for resuscitation of starvation- and low-temperature-induced nonculturable cells of Aeromonas hydrophila

Wai

Mizunoe

Takade

et al. 2000

Archives of Microbiology

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Like many other gram-negative bacteria, starved cells of Aeromonas hydrophila can be induced into a viable but nonculturable (VBNC) state by incubation at low temperature, as shown here by using various bacterial enumeration methods. Starved A. hydrophila strain HR7 cells at 4 degrees C reached the nonculturable stage in about 45 days. The cells were resuscitated by either a solid medium resuscitation method, using solid agar amended with H2O2-degrading agents, catalase or sodium pyruvate, or a liquid medium resuscitation method, by incubating nonculturable cells in liquid media containing these compounds before spreading onto plates. The liquid medium resuscitation method using catalase resulted in nearly complete recovery of nonculturable cells.

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Section: Resultsmentioning

confidence: 86%

A comparison of solid and liquid media for resuscitation of starvation- and low-temperature-induced nonculturable cells of Aeromonas hydrophila

Wai

Mizunoe

Takade

et al. 2000

Archives of Microbiology

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“…Reproducibility of results Lemcke & White (1959) demonstrated that the heat resistance of bacterial cells varied during the growth cycle with stationary phase cells being measureably more heat resistant. Pellon & Gomez (1979) found that batches of agar contained materials toxic to heated Escherichia coli. In order to obtain reproducible results it was, therefore, considered necessary to standardize the growth conditions and the time of incubation of the organisms to be heated and also wherever possible to use the same batches of plate count agar.…”

Section: Determination Ofthe Death Rates Of'sulmonella Serotypesmentioning

confidence: 99%

The Effects of pH and Levels of Organic Matter on the Death Rates of Salmonellas in Chicken Scald‐tank Water

Humphrey

1981

Journal of Applied Bacteriology

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The immersion scalding of chicken carcases at 52°C for 2.5 min was found to result in the accumulation of bacteria and organic matter in the water. Disassociation of ammonium urate present in chicken faeces caused the pH value of the water to fall rapidly from 8.4 to 6.0. This pH was maintained for most of the time the tank was in operation. The change in pH value greatly increased the heat resistance of some common salmonella serotypes with a strain of Salmonella typhimurium being the most resistant, having a D52"C value of 34.5 min. The pH value of the water was found to be close to the optimum for the heat resistance of salmonellas. In scald water at pH 5.9-60 the death rate of Salm. typhimurium was shown to be independent of organic matter levels indicating that pH was the major influence on heat resistance.

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“…Typhimurium, with no substantial difference observed in the case of LB. Factors involved may include the physical, chemical and biological effects of agar, such as nutritional diffusion, oxygen supply and contaminating substances (Corry et al, 2007; Ouyang et al, 2018; Pellon & Gomez, 1979; Stephens et al, 2000; Thomas et al, 2014). Although we cannot specify the target factor at present, one or some of these may affect the generation and degree of injury and the differences between solid and liquid media in heated E. coli cells, as suggested by other investigators (Mackey & Derrick, 1982; Stephens et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of distinct modes of oxidative secondary injury generated in heat-treated cells of Escherichia coli with solid/liquid and complex/semi-synthetic media sets

Wada

Iwata

et al. 2022

Journal of Applied Microbiology

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Aims:To characterize and evaluate oxidative secondary injury generated in heattreated Escherichia coli cells during recovery cultivation either on agar or in a broth of a semi-synthetic enriched M9 (EM9) medium and a complex Luria broth (LB) medium with different types of antioxidants.Methods and Results: E. coli cells grown in the EM9 and LB broth were heated at 50°C in a buffer (pH 7.0). Heated cells were recovered on the same kind of agar medium as that used for growth, with or without different antioxidants. Although these antioxidants mostly protected the cells from oxidative secondary injury on the recovery media, sodium thiosulphate and sodium pyruvate were most protective on EM9 and LB agars, respectively. Determination of viability using the most probable number and growth delay analysis methods showed significant reductions in the protective effects of antioxidants in the EM9 and LB media. Conclusion:Oxidative secondary injury generated in heated E. coli cells was found to be qualitatively and quantitatively diverse under cellular and environmental conditions.Significance and impact of the study: Our results suggest that different modes of oxidation should be considered in viability determination and injured cell enumeration of heat-treated cells.

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A Note on the Effect of Different Types of Agar on the Recovery of Heated Escherichia coli K‐12

Cited by 4 publications

References 10 publications

A comparison of solid and liquid media for resuscitation of starvation- and low-temperature-induced nonculturable cells of Aeromonas hydrophila

A comparison of solid and liquid media for resuscitation of starvation- and low-temperature-induced nonculturable cells of Aeromonas hydrophila

The Effects of pH and Levels of Organic Matter on the Death Rates of Salmonellas in Chicken Scald‐tank Water

Evaluation of distinct modes of oxidative secondary injury generated in heat-treated cells of Escherichia coli with solid/liquid and complex/semi-synthetic media sets

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