A note on the universal calibration curve for gel permeation chromatography

Weiss, Alan R.; Cohn-Ginsberg, Elizabeth

doi:10.1002/pol.1969.110070510

Cited by 72 publications

(2 citation statements)

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“…12,[18][19][20] The common drawback of these methods is that they require something what is often unavailable or doubtful. 12,[18][19][20] The common drawback of these methods is that they require something what is often unavailable or doubtful.…”

Section: Theory and Basic Principlesmentioning

confidence: 99%

“…Literature shows various other methods of the calibration of SEC columns by means of polydisperse standards or the recalculation of the calibration established with narrow standards to a calibration valid for a polymer of different chemical composition. 12,[18][19][20] The common drawback of these methods is that they require something what is often unavailable or doubtful. Well characterized polydisperse standards are rare, and in addition, the calibration based on them may be less accurate than that based on narrow standards.…”

Section: Characterization Of Molar Mass Distribution By Secmentioning

confidence: 99%

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Truths and myths about the determination of molar mass distribution of synthetic and natural polymers by size exclusion chromatography

Podzimek

2013

J of Applied Polymer Sci

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This article discusses various aspects of the determination of molar mass distribution by means of size exclusion chromatography (SEC) in various application modes. The effects of erroneous specific refractive index increment (dn/dc), branching, column performance, and enthalpic interactions on the results obtained by different SEC techniques are discussed. Combination of SEC and a light scattering detector represents the most direct way to the molar mass distribution of all natural and synthetic polymers as it completely eliminates the need for column calibration and to a certain extent eliminates the dependence of the obtained results on some operational variables such as flow rate, temperature, or injected mass. A multiangle light scattering (MALS) photometer has become the most frequently used light scattering detector capable of determination of molecular size as another important polymer characteristic. This article contrasts SEC-MALS method with other application modes of SEC from the viewpoint of some frequent confusions and misunderstandings.

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Section: Theory and Basic Principlesmentioning

confidence: 99%

Section: Characterization Of Molar Mass Distribution By Secmentioning

confidence: 99%

Truths and myths about the determination of molar mass distribution of synthetic and natural polymers by size exclusion chromatography

Podzimek

2013

J of Applied Polymer Sci

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Chromatography of Polymers

Radke

2007

Macromolecular Engineering

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Application of the Π‐theorem to the determination of molecular mass distribution in individual unfractionated polymer samples. I. Basic considerations

Kalfus

1981

Acta Polymerica

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The theoretical principles of the method are summarized in 25 equations, resulting from t h e application of the ITtheorem t o the calibration of the sedimentation coefficientmolecular mass and abscissa of the gel-permeation chromatogrammolecular mass relations. Turbidimetric titration was considered. Only t w o independently determined moments, e.g. Bw and H,, are needed for the calibration. The method was developed for investigations in preparing new polymers. Die Anwendung des Ii-Theorems zur Bestimmung der Molekiilinassenoerteilung in individuellen unfraktionierten Polymerproben. I . Theoretische GrudlagenDie theoretischen Grundlagen des Bestimmungsverfahrens werden in 25 Gleichungen angegeben. Die Gleichungen wurden aus der Anwendung des 17-Theorems auf die Eichung der Beziehungen zwischen Sedimentationskoeffizient und Molekiilmasse sowie zwischen der Abszisse des Gelchromatogramms und der Molekulmasse abgeleitet. Die turbidimetrische Titration wird betrachtet. Nur zwei unabhiingig bestimmte Momente, z. B. zw und z,,, sind fur die Eichung notwendig. Die Methode wurde fur Untersucbungen bei der Entwicklung neuer Polymere ausgearbeitet. IIp w n e n u e n-meopewiIE onpetleaenurn Nonexyaspnomaccoeozo pacnpetlemnus e otlunornbsx npo6ax nomrepoe I I . Ocnosnbce c o o 6 p~e n u s . OcHoBHue TeopeTmecKHe COObpaXeHHH no BbmeynoMsHyToMy onpegenemm, n p e~~i a 3~a q e~~o~y gns mccnego-BaHIifi B XOAe pa3pa6OTKH HOBMX nOJIHMepOB, npegCTaBJIeHM B 25 YpaBHeHHHX, BbIBeAeHHbIX IIj'TeM npUMeHeHHfI ?L-TeOpeMbI K HaJIIlbpOBKe COOTHOIUeHHfi KOB@@liqHeHT CenliMeHTaqHU -MOJleKyJrHpHaR MaCCa li a6cq~cca XPO-MaTOl' paMMbI -MOJ' leKyJ' lHpHaH MaCCa. ObCyWgeHO TypbHAEiMeTp~'4eCK~e THTpOBaHHe. A J I H KaJIHbpOBKH TpebymT-CH TOJIbKO ABa He3aBHCEIMO OIIpeAeJIeHHMe MOMeHThI, HaIIpHMep, &fw Y,.

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A note on the universal calibration curve for gel permeation chromatography

Cited by 72 publications

References 3 publications

Truths and myths about the determination of molar mass distribution of synthetic and natural polymers by size exclusion chromatography

Truths and myths about the determination of molar mass distribution of synthetic and natural polymers by size exclusion chromatography

Chromatography of Polymers

Application of the Π‐theorem to the determination of molecular mass distribution in individual unfractionated polymer samples. I. Basic considerations

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