A novel 17β-hydroxysteroid dehydrogenase in Rhodococcus sp. P14 for transforming 17β-estradiol to estrone

Ye, Xueying; Wang, Hui; Kan, Jie; Li, Jin; Huang, Tongwang; Guan, Xiong; Huang, Zhong

doi:10.1016/j.cbi.2017.06.010

Cited by 33 publications

(14 citation statements)

References 35 publications

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“…HSD plays a major role in the dehydrogenation of steroid hormones. Both 17 β -HSD recombinant strains and purified proteins can efficiently convert E2 into E1 by C17 dehydrogenation [ 49 ], and a similar role for HSD has been reported in some fungi and Pseudomonas sp. [ 50 – 52 ].…”

Section: Resultsmentioning

confidence: 92%

“…DSSKY-A-001 was cultured with estradiol as the sole carbon source, Huo found that the dioxygenase gene, RspwpGM002188 ; the catechol 1,2 dioxygenase gene, RspwpGM001668 ; and the 7 α -hydroxysteroid dehydrogenase gene, RspwpGM001333 , were involved in different E2 degradation stages and had high expression levels [ 37 ]. Ye et al [ 38 ] used plasmid construction and heterologous expression to demonstrate that HSD , which encodes a 17 β -hydroxysteroid dehydrogenase in Rhodococcus sp. P14, was related to the bacterium's E2 degradation ability.…”

Section: Introductionmentioning

confidence: 99%

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The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001

Tian

Meng

et al. 2020

International Journal of Genomics

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Estrogen contamination is recognized as one of the most serious environmental problems, causing widespread concern worldwide. Environmental estrogens are mainly derived from human and vertebrate excretion, drugs, and agricultural activities. The use of microorganisms is currently the most economical and effective method for biodegradation of environmental estrogens. Rhodococcus equi DSSKP-R-001 (R-001) has strong estrogen-degrading capabilities. Our study indicated that R-001 can use different types of estrogen as its sole carbon source for growth and metabolism, with final degradation rates above 90%. Transcriptome analysis showed that 720 (E1), 983 (E2), and 845 (EE2) genes were significantly upregulated in the estrogen-treated group compared with the control group, and 270 differentially expressed genes (DEGs) were upregulated across all treatment groups. These DEGs included ABC transporters; estrogen-degrading genes, including those that perform initial oxidation and dehydrogenation reactions and those that further degrade the resulting substrates into small molecules; and metabolism genes that complete the intracellular transformation and utilization of estrogen metabolites through biological processes such as amino acid metabolism, lipid metabolism, carbohydrate metabolism, and the tricarboxylic acid cycle. In summary, the biodegradation of estrogens is coordinated by a metabolic network of estrogen-degrading enzymes, transporters, metabolic enzymes, and other coenzymes. In this study, the metabolic mechanisms by which Rhodococcus equi R-001 degrades various estrogens were analyzed for the first time. A new pollutant metabolism system is outlined, providing a starting point for the construction of engineered estrogen-degrading bacteria.

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Section: Resultsmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001

Tian

Meng

et al. 2020

International Journal of Genomics

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“…These compounds can interfere with hormonal systems and adversely impact sexual development, reproduction, the nervous system, and immunity of both wildlife and humans, even at concentrations as low as parts per million (ppm) and parts per billion (ppb) (Su et al, 2020;Viguié et al, 2020). For example, 17β-estradiol (E2) is a representative estrogen that causes serious environmental and global issues owing to its induction of reproductive disorders, immune deficiencies, and even carcinogenic risks (Ye et al, 2017;Du et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Introducing a Thermo-Alkali-Stable, Metallic Ion-Tolerant Laccase Purified From White Rot Fungus Trametes hirsuta

Cao

et al. 2021

Front. Microbiol.

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This study introduces a valuable laccase, designated ThLacc-S, purified from white rot fungus Trametes hirsuta. ThLacc-S is a monomeric protein in nature with a molecular weight of 57.0 kDa and can efficiently metabolize endocrine disrupting chemicals. The enzyme was successfully purified to homogeneity via three consecutive steps consisting of salt precipitation and column chromatography, resulting in a 20.76-fold increase in purity and 46.79% yield, with specific activity of 22.111 U/mg protein. ThLacc-S was deciphered as a novel member of the laccase family and is a rare metalloenzyme that contains cysteine, serine, histidine, and tyrosine residues in its catalytic site, and follows Michaelis-Menten kinetic behavior with a Km and a kcat/Km of 87.466 μM and 1.479 s–1μM–1, respectively. ThLacc-S exerted excellent thermo-alkali stability, since it was markedly active after a 2-h incubation at temperatures ranging from 20 to 70°C and retained more than 50% of its activity after incubation for 72 h in a broad pH range of 5.0–10.0. Enzymatic activities of ThLacc-S were enhanced and preserved when exposed to metallic ions, surfactants, and organic solvents, rendering this novel enzyme of interest as a green catalyst for versatile biotechnological and industrial applications that require these singularities of laccases, particularly biodegradation and bioremediation of environmental pollutants.

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“…In human, multiple 17β-hydroxysteroid dehydrogenases (17β-HSDs) have been confirmed to catalyze the oxidation/reduction reaction of 17β-estradiol and estrone (Labrie et al 1997). In bacteria, several enzymes belonging to the short-chain dehydrogenase/reductase (SDR) superfamily were also identified with similar function (Chen et al 2017;Ye et al 2017;Yu et al 2015). In D. actinosclerus SJTR1, genome analysis showed that there were 18 genes encoding SDRs, probably contributing to the transformation from 17β-estradiol to estrone (Table S4).…”

Section: Introductionmentioning

confidence: 99%

Identification and genome analysis of Deinococcus actinosclerus SJTR1, a novel 17β-estradiol degradation bacterium

2018

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Biodegradation with microorganisms is considered as an efficient strategy to remove the environmental pollutants. In this work, Deinococcus actinosclerus SJTR1 isolated from the wastewater was confirmed with great degradation capability to 17β-estradiol, one typical estrogen chemical. It could degrade nearly 90% of 17β-estradiol (10 mg/L) in 5 days and transform it into estrone; its degradation kinetics fitted for the first-order kinetic equation. The whole genome sequence of D. actinosclerus SJTR1 was obtained and annotated, containing one chromosome (3,315,586 bp) and four plasmids (ranging from 17,267 bp to 460,244 bp). A total of 3913 CDSs and 73 RNA genes (including 12 rRNA genes, 50 tRNA genes, and 11 ncRNA genes) were identified in its whole genome sequence. On this basis, a series of potential genes involved in steroid metabolism and stress responses of D. actinosclerus SJTR1 were predicted. It is the first report of Deinococcus strain with the degradation capability to estrogens. This work could enrich the genome sources of the estrogen-degrading strains and promote the degradation mechanism study of 17β-estradiol in bacteria.

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A novel 17β-hydroxysteroid dehydrogenase in Rhodococcus sp. P14 for transforming 17β-estradiol to estrone

Cited by 33 publications

References 35 publications

The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001

The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001

Introducing a Thermo-Alkali-Stable, Metallic Ion-Tolerant Laccase Purified From White Rot Fungus Trametes hirsuta

Identification and genome analysis of Deinococcus actinosclerus SJTR1, a novel 17β-estradiol degradation bacterium

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