A novel 9×9 map-based solvent selection strategy for targeted counter-current chromatography isolation of natural products

Liang, Junling; Meng, Jie; Wu, Dazhi; Guo, Mengzhe; Wu, Shuai

doi:10.1016/j.chroma.2015.04.043

Cited by 48 publications

(24 citation statements)

References 44 publications

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“…Some studies were performed using FR purchased from the Pharmazeutische Biologie at the Universit€ at Bonn. In addition, FR was also isolated from the roots of Ardisia crenata sims using the targeted counter-current chromatography method with minor modifications (34,35). In brief, the dry powder of the roots of Ardisia crenata sims was first extracted with 95% ethanol, then the ethanol extract was partitioned by n-butanol-water.…”

Section: Fr900359mentioning

confidence: 99%

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Effects of Oncogenic Gαq and Gα11 Inhibition by FR900359 in Uveal Melanoma

Lapadula

Farías

Randolph

et al. 2019

Molecular Cancer Research

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Uveal melanoma is the most common intraocular tumor in adults and often metastasizes to the liver, leaving patients with few options. Recurrent activating mutations in the G proteins, Gαq and Gα11, are observed in approximately 93% of all uveal melanomas. While therapeutic intervention of downstream Gαq/11 targets has been unsuccessful in treating uveal melanoma, using in vitro [35S]GTPγS binding and cell signaling assays we have found that the Gαq/11 inhibitor, FR900359 (FR), effectively inhibits oncogenic Gαq/11 signaling in uveal melanoma cells expressing either mutant Gαq or Gα11. Inhibition of oncogenic Gαq/11 by FR results in cell cycle arrest and induction of apoptosis. Furthermore, colony formation is prevented by FR treatment of uveal melanoma cells in 3D‐cell culture, providing promise for future in vivo studies. This suggests direct inhibition of activating Gαq/11 mutants may be a potential means of treating uveal melanoma. Support or Funding Information Dr. Ralph and Marian Falk Medical Research Trust and NIH award F31 CA225064 This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

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Section: Fr900359mentioning

confidence: 99%

“…A positive ESI-MS spectra showed the characteristic molecular ions at m/z 1002.27 [MþH] þ and 1024. 35 [MþNa] þ implying its molecular formula (C 49 H 75 N 7 O 15 ). 1 H NMR and 13 C NMR data were in close agreement with previous data (29).…”

Section: Fr900359mentioning

confidence: 99%

Effects of Oncogenic Gαq and Gα11 Inhibition by FR900359 in Uveal Melanoma

Lapadula

Farías

Randolph

et al. 2019

Molecular Cancer Research

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“…Choosing a suitable solvent system is an extremely important step for the separation of compounds by CCC, especially in the case of complex mixtures, such as plant extracts 8,27 . Literature reports n-hexane-ethyl acetatemethanol-water (HEMWat) [28][29][30][31]25 solvent system as the most suitable for isolation of flavonoid compounds.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Phenolic Compounds from Eryngium glaziovianum Stem by Countercurrent Chromatography with Off-line HPLC-DAD Detection

Wuillda

Trovó²,

Martins

et al. 2018

Rev. Virtual Quim.

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Isolamento e Identificação de Substâncias Fenólicas do Caule de Eryngium glaziovianum por Cromatografia Contracorrente com Detecção HPLC-DAD Off-line Resumo: O extrato bruto hidroalcoólico do caule da espécie Eryngium glaziovianum (Apiaceae) foi fracionado por Cromatografia Contracorrente (CCC) usando um sistema de solventes bifásico composto por n-hexano-acetato de etila-metanol-água (0,5: 6: 0,5: 6, v/v) no modo de eluição normal. As frações obtidas por CCC foram analisadas e parcialmente caracterizadas por HPLC-DAD off-line e as principais substâncias foram identificados por ESI-MS/MS e RMN uni e bidimensional. Esta técnica permitiu o isolamento de uma mistura de isômeros dos flavonoides quercetina 3-O-β-D-galactopiranosídeo e quercetina 3-O-β-D glucopiranosídeo, além dos ácidos fenólicos clorogênico e 3,4-dicafeoilquínico. A CCC provou ser uma técnica eficiente e rápida para a separação e purificação de metabolitos secundários isoméricos e sua combinação com a detecção HPLC-DAD off-line permitiu a análise precisa das frações e a caracterização parcial das substâncias fenólicas.

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“…And then when the proportion was 8:2:8:2, peak 1 had a suitable K value 1.54 (0.4 < K < 2.5) . In addition, this system has short settling time and is suitable to achieve high retention of stationary phase in CCC separation . So the solvent system (HEMWat, 8:2:8:2, v/v) was selected for the separation of peak 1.…”

Section: Resultsmentioning

confidence: 99%

Preparative isolation of a cytotoxic principle of a forest mushroom Suillus luteus by sodium dodecyl sulfate based “salting‐in” countercurrent chromatography

Yang

2016

J of Separation Science

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In the course of screening new anticancer natural products, an edible forest mushroom Suillus luteus (L. Ex Franch). Gray was found to have potent cytotoxicity against several human cancer cells. However, the lipophilic sample made some countercurrent chromatography solvent systems emulsify, which caused difficulties in the separation of its cytotoxic components. Here, we found that the addition of an organic salt sodium dodecyl sulfate could efficiently shorten the settling time of the mushroom sample solutions by eliminating the emulsification of two-phase solvent systems. Moreover, we found that sodium dodecyl sulfate could play a new "salting-in" role and made the partition coefficients of the solutes decrease with the increased concentrations. Thus, a sodium dodecyl sulfate based salting-in countercurrent chromatography method has been successfully established for the first time for preparative isolation of a cytotoxic principle of the mushroom. The active component was identified as isosuillin. Whole results indicated that sodium dodecyl sulfate could be used as an efficient salting-in reagent for two-phase solvent system selection and targeted countercurrent chromatography isolation. It is very useful for current natural products isolation and drug discovery.

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A novel 9×9 map-based solvent selection strategy for targeted counter-current chromatography isolation of natural products

Cited by 48 publications

References 44 publications

Effects of Oncogenic Gαq and Gα11 Inhibition by FR900359 in Uveal Melanoma

Effects of Oncogenic Gαq and Gα11 Inhibition by FR900359 in Uveal Melanoma

Isolation and Identification of Phenolic Compounds from Eryngium glaziovianum Stem by Countercurrent Chromatography with Off-line HPLC-DAD Detection

Preparative isolation of a cytotoxic principle of a forest mushroom Suillus luteus by sodium dodecyl sulfate based “salting‐in” countercurrent chromatography

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