A novel, abundant sodium channel expressed in neurons and glia

Schaller, K; D, Krzemień; Yarowsky, PJ; Krueger, Bruce K.; Caldwell, John H.

doi:10.1523/jneurosci.15-05-03231.1995

Cited by 218 publications

(173 citation statements)

References 45 publications

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“…In the adult, both zebrafish genes are predominantly expressed in neural tissue (brain, spinal cord and eye), similar to the expression pattern of SCN8A in mammals (Krzemien et al 2000;Schaller et al 1995;Tzoumaka et al 2000). Interestingly our RT-PCR analyses also reveal expression in skeletal and cardiac muscle, as recently reported for mammalian SCN8A in cardiac tissue (Dhar Malhotra et al 2001;Maier et al 2002).…”

Section: Scna Gene Expression In Teleosts and Mammalssupporting

confidence: 87%

Gene Duplications and Evolution of Vertebrate Voltage-Gated Sodium Channels

Novak¹,

Jost

et al. 2006

J Mol Evol

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Abstract.Voltage-gated sodium channels underlie action potential generation in excitable tissue.To establish the evolutionary mechanisms that shaped the vertebrate sodium channel a-subunit (SCNA) gene family and their encoded Na v 1 proteins, we identified all SCNA genes in several teleost species. Molecular cloning revealed that teleosts have eight SCNA genes, comparable to the number in another vertebrate lineage, mammals.Prior phylogenetic analyses had indicated that teleosts and tetrapods share four monophyletic groups of SCNA genes and that tandem duplications selectively expanded the number of genes in two of the four mammalian groups. However, the number of genes in each group varies between teleosts and tetrapods suggesting different evolutionary histories in the two vertebrate lineages. Our findings from phylogenetic analysis and chromosomal mapping of Danio rerio genes indicate that tandem duplications are an unlikely mechanism for generation of the extant teleost SCNA genes. Instead, analysis of other closely mapped genes in D. rerio supports the hypothesis that a whole genome duplication was involved in expansion of the SCNA gene family in teleosts. Interestingly, despite their different evolutionary histories, mRNA analyses demonstrated a conservation of expression patterns for SCNA orthologues in teleosts and tetrapods, suggesting functional conservation.

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Section: Scna Gene Expression In Teleosts and Mammalssupporting

confidence: 87%

Gene Duplications and Evolution of Vertebrate Voltage-Gated Sodium Channels

Novak¹,

Jost

et al. 2006

J Mol Evol

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“…SCN8A is expressed broadly throughout the central and peripheral nervous systems during early embryogenesis (Schaller et al, 1995;Krzemien et al, 2000;Schaller and Caldwell, 2000). Previous work has shown that zebrafish scn8aa mRNA expression begins early (16 hpf) and occurs in many regions of both the central and peripheral nervous systems (Tsai et al, 2001).…”

Section: Scn8aa and Scn8ab Gene Expressionmentioning

confidence: 99%

Embryonic and larval expression of zebrafish voltage‐gated sodium channel α‐subunit genes

Novak¹,

Taylor²,

Pineda³

et al. 2006

Developmental Dynamics

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Whereas it is known that voltage-gated calcium channels play important roles during development, potential embryonic roles of voltage-gated sodium channels have received much less attention. Voltagegated sodium channels consist of pore-forming ␣-subunits (Na v 1) and auxiliary ␤-subunits. Here, we report the embryonic and larval expression patterns for all eight members of the gene family (scna) coding for zebrafish Na v 1 proteins. We find that each scna gene displays a distinct expression pattern that is temporally and spatially dynamic during embryonic and larval stages. Overall, our findings indicate that scna gene expression occurs sufficiently early during embryogenesis to play developmental roles for both muscle and nervous tissues.

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“…Na v 1.6 (also termed SCN8A or PN4) is a sodium channel that is expressed throughout the CNS and the PNS (Burgess et al, 1995;Schaller et al, 1995) and is abundant at the axon initial segment, at the nodes of Ranvier Krzemien et al, 2000;Tzoumaka et al, 2000;Boiko et al, 2001), and along nonmyelinated axons of the PNS . Na v 1.6 produces a fast inactivating, TTX-sensitive (TTX-S) sodium current (Dietrich et al, 1998;Smith et al, 1998;Herzog et al, 2003b).…”

Section: Introductionmentioning

confidence: 99%

Fibroblast Growth Factor Homologous Factor 2B: Association with Na_v1.6 and Selective Colocalization at Nodes of Ranvier of Dorsal Root Axons

Wittmack¹,

Rush²,

Craner³

et al. 2004

J. Neurosci.

129

174

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Voltage-gated sodium channels interact with cytosolic proteins that regulate channel trafficking and/or modulate the biophysical properties of the channels. Na v 1.6 is heavily expressed at the nodes of Ranvier along adult CNS and PNS axons and along unmyelinated fibers in the PNS. In an initial yeast two-hybrid screen using the C terminus of Na v 1.6 as a bait, we identified FHF2B, a member of the FGF homologous factor (FHF) subfamily, as an interacting partner of Na v 1.6. Members of the FHF subfamily share ϳ70% sequence identity, and individual members demonstrate a cell-and tissue-specific expression pattern. FHF2 is abundantly expressed in the hippocampus and DRG neurons and colocalizes with Na v 1.6 at mature nodes of Ranvier in myelinated sensory fibers in the dorsal root of the sciatic nerve. However, retinal ganglion cells and spinal ventral horn motor neurons show very low levels of FHF2 expression, and their axons exhibit no nodal FHF2 staining within the optic nerve and ventral root, respectively. Thus, FHF2 is selectively localized at nodes of dorsal root sensory but not ventral root motor axons. The coexpression of FHF2B and Na v 1.6 in the DRG-derived cell line ND7/23 significantly increases the peak current amplitude and causes a 4 mV depolarizing shift of voltage-dependent inactivation of the channel. The preferential expression of FHF2B in sensory neurons may provide a basis for physiological differences in sodium currents that have been reported at the nodes of Ranvier in sensory versus motor axons.

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A novel, abundant sodium channel expressed in neurons and glia

Cited by 218 publications

References 45 publications

Gene Duplications and Evolution of Vertebrate Voltage-Gated Sodium Channels

Gene Duplications and Evolution of Vertebrate Voltage-Gated Sodium Channels

Embryonic and larval expression of zebrafish voltage‐gated sodium channel α‐subunit genes

Fibroblast Growth Factor Homologous Factor 2B: Association with Na_v1.6 and Selective Colocalization at Nodes of Ranvier of Dorsal Root Axons

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A novel, abundant sodium channel expressed in neurons and glia

Cited by 218 publications

References 45 publications

Gene Duplications and Evolution of Vertebrate Voltage-Gated Sodium Channels

Gene Duplications and Evolution of Vertebrate Voltage-Gated Sodium Channels

Embryonic and larval expression of zebrafish voltage‐gated sodium channel α‐subunit genes

Fibroblast Growth Factor Homologous Factor 2B: Association with Nav1.6 and Selective Colocalization at Nodes of Ranvier of Dorsal Root Axons

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Fibroblast Growth Factor Homologous Factor 2B: Association with Na_v1.6 and Selective Colocalization at Nodes of Ranvier of Dorsal Root Axons