A novel acetylcholinesterase biosensor based on ionic liquids-AuNPs-porous carbon composite matrix for detection of organophosphate pesticides

Wei, Min; Wang, Jingjing

doi:10.1016/j.snb.2015.01.112

Cited by 110 publications

(45 citation statements)

References 46 publications

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“…It has been found the AChE inhibited by paraoxon can resume 96.74 % original activity by immersing in 0.1 M PBS (pH 7.4) for 20 min. This method is simple and reliable, comparing with the previously reported nucleophilic compounds as a reagent of reactivation .…”

Section: Resultssupporting

confidence: 92%

Novel Acetylcholinesterase Biosensor for Detection of Paraoxon Based on Holey Graphene Oxide Modified Glass Carbon Electrode

Zhao

Han

et al. 2018

Electroanalysis

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Holey graphene oxide (HGO) has been synthesized by one step hydrothermal method and applied to construct a highly sensitive acetylcholinesterase (AChE) biosensor. The developed system is characterized by using SEM, TEM, nitrogen adsorption‐desorption, Raman, and electrochemical impedance spectroscopy. It is proved that HGO can effectively immobilize AChE and accelerate electron transfer rate. The prepared biosensor AChE/HGO/glass carbon electrode (GCE) shows excellent affinity to the acetylthiocholine chloride (ATCl) with a Michaelis‐Menten constant value of 0.23 mM. Under optimum conditions, AChE/HGO/GCE can effectively detect paraoxon with a linear ranged from 10 to 45 ng/mL and a detection limit of 1.58 ng/mL. Furthermore, the prepared AChE/HGO/GCE biosensor demonstrates excellent stability and reproducibility, which provides a reliable method for the detection of organophosphate pesticides. The developed biosensor is used for detecting paraoxon in real samples of lettuce and cabbage and shows a satisfactory recovery.

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Section: Resultssupporting

confidence: 92%

Novel Acetylcholinesterase Biosensor for Detection of Paraoxon Based on Holey Graphene Oxide Modified Glass Carbon Electrode

Zhao

Han

et al. 2018

Electroanalysis

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“…The maximum current for the biosensor response was observed at pH 7.5-8.0. This optimal pH of poly(TTP)/AChE/GCE is close to that of AChE-based biosensors reported previously (Chauhan and Pundir 2011;Wei and Wang 2015). However, 0.1 M of phosphate buffer with pH of 7.4 was selected for all electrochemical experiments due to the physiological pH.…”

Section: Effect Of Ph and Temperature On Poly(ttp)/ache/gce Responsesupporting

confidence: 73%

Electrochemical detection of malathion pesticide using acetylcholinesterase biosensor based on glassy carbon electrode modified with conducting polymer film

Güler

Türkoğlu

Kıvrak

2016

Environ Sci Pollut Res

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Acetylcholinesterase (AChE) biosensor based on conducting poly([2,2̍';5̍' 2″]-terthiophene-3̍-carbaldehyde) (PTT) modified glassy carbon electrode (GCE) was constructed. AChE was immobilized on PTT film surface through the covalent bond between aldehyde and amino groups. The properties of PTT modified GCE were studied using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). The biosensor showed an oxidation peak at +0.83 V related to the oxidation of thiocholine, hydrolysis product of acetylthiocholine iodide (ATCI), catalyzed by AChE. The optimum current response of the biosensor was observed at pH 7.5-8.0, 40 °C and 120 U/cm(2) of AChE concentration. The biosensor showed a high sensitivity (183.19 μA/mM), a linear range from 0.015 to 1.644 mM, and a good reproducibility with 1.7 % of relative standard deviation (RSD). The biosensor showed a good stability. The interference of glycin, ascorbic acid, histidine, uric acid, dopamine, and arginine on the biosensor response was studied. An important analytical response from these inteferents that overlaps the biosensor response was not observed. The inhibition rate of malathion as a model pesticide was proportional to its concentrations from 9.99 to 99.01 nM. The detection limit was 4.08 nM.

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“…Wei et al. reported ion liquids‐AuNPs‐porous carbon composite modified electrode for the detection of OPs, which showed excellent conductivity, lower detection limit and good reproducibility . However, much effort that contributes the biosensor performance and further enhancement of electrical conductivity are still required to develop high performance biosensors.…”

Section: Introductionmentioning

confidence: 62%

Mesoporous Bimetallic PtPd Nanoflowers as a Platform to Enhance Electrocatalytic Activity of Acetylcholinesterase for Organophosphate Pesticide Detection

Zhou

et al. 2018

Electroanalysis

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Mesoporous bimetallic PtPd nanoflowers (MPtPdN) were synthesized by a surfactant‐directing method. The MPtPdN was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and wide‐angle X‐ray diffraction (XRD). The use of MPtPdN as a platform for the indirect electrochemical detection of organophosphate pesticides (OPs) via enzymatic inhibition pathway was demonstrated. Due to the nanostructure of MPtPdN and the synergy effect of the noble metal nanoparticles, a novel and sensitive acetylcholinesterase (AChE) biosensor for OPs detections based on MPtPdN and enzyme inhibition was prepared. The inhibition of omethoate exhibited a linear relationship from 4.7×10−11 to 4.7×10−8 M with a detection limit of 1.7×10−12 M (S/N=3). The proposed AChE biosensor was reliable and can be used to measure the concentration of omethoate in different spiked samples with high accuracy and satisfactory recovery. The preparation of biosensors based on the MPtPdN can be further extended to construct many other important enzyme biosensors.

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A novel acetylcholinesterase biosensor based on ionic liquids-AuNPs-porous carbon composite matrix for detection of organophosphate pesticides

Cited by 110 publications

References 46 publications

Novel Acetylcholinesterase Biosensor for Detection of Paraoxon Based on Holey Graphene Oxide Modified Glass Carbon Electrode

Novel Acetylcholinesterase Biosensor for Detection of Paraoxon Based on Holey Graphene Oxide Modified Glass Carbon Electrode

Electrochemical detection of malathion pesticide using acetylcholinesterase biosensor based on glassy carbon electrode modified with conducting polymer film

Mesoporous Bimetallic PtPd Nanoflowers as a Platform to Enhance Electrocatalytic Activity of Acetylcholinesterase for Organophosphate Pesticide Detection

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