A Novel Agarase, Gaa16B, Isolated from the Marine Bacterium Gilvimarinus agarilyticus JEA5, and the Moisturizing Effect of Its Partial Hydrolysis Products

Lee, Jehee; Jo, Eunyoung; Eom, Tae-Yang; Gang, Yehui; Kang, Yoon-Hyeok; Marasinghe, Svini Dileepa; Hettiarachchi, Sachithra Amarin; Kang, Do‐Hyung; Oh, Chulhong

doi:10.3390/md20010002

Cited by 9 publications

(5 citation statements)

References 34 publications

(40 reference statements)

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“…Some Gilvimarinus strains have been reported to exhibit the capability to degrade marine algal polysaccharides, such as agar, cellulose, carrageenan and laminarin ( Du et al, 2009 ; Kim et al, 2011 ; Cheng et al, 2015 ; Kouzui et al, 2016 ; Kouzuma and Fujii, 2018 ; Lee et al, 2018 , 2021 ). Based on the genomic annotation and dbCAN analysis, the genome of strain HB14 T encodes 120 GHs, 23 polysaccharide lyases (PL), 38 carbohydrate esterases (CE) and 57 carbohydrate-binding modules (CBM), distributed in 40, 14, 12 and 24 families, respectively ( Figure 2B ).…”

Section: Resultsmentioning

confidence: 99%

“…Limnobacter litoralis NBRC 105857 T was used as the outgroup. 2018; Lee et al, 2018Lee et al, , 2021. Based on the genomic annotation and dbCAN analysis, the genome of strain HB14 T encodes 120 GHs, 23 polysaccharide lyases (PL), 38 carbohydrate esterases (CE) and 57 carbohydrate-binding modules (CBM), distributed in 40, 14, 12 and 24 families, respectively (Figure 2B).…”

Section: Morphological Physiological and Biochemical Characteristicsmentioning

confidence: 99%

“…All species in the genus Gilvimarinus have been isolated from coastal environments, including seawater, sea sand, seaweed and debris ( Du et al, 2009 ; Kim et al, 2011 ; Cheng et al, 2015 ; Kouzui et al, 2016 ), where phytoplankton blooms frequently occur. Furthermore, type strains of all Gilvimarinus species were reported to possess the capability to degrade algal polysaccharides, especially agar and cellulose ( Du et al, 2009 ; Kim et al, 2011 ; Cheng et al, 2015 ; Kouzui et al, 2016 ; Kouzuma and Fujii, 2018 ; Lee et al, 2018 , 2021 ). Thus, they likely play important ecological roles in seaweed decomposition in coastal marine environments.…”

Section: Introductionmentioning

confidence: 99%

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Gilvimarinus xylanilyticus sp. nov., a novel 1,3-xylanase-secreting bacterium isolated from a marine green alga

Zhang

Sun

et al. 2022

Front. Microbiol.

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1,3-xylan, an important organic carbon in the ocean, is peculiar to marine algae. 1,3-xylanase-secreting bacteria and their extracellular 1,3-xylanases play pivotal roles in the degradation and biomass conversion of 1,3-xylan. However, only a few 1,3-xylanase-secreting bacteria and 1,3-xylanases have been reported. Here, we identified a novel marine bacterium capable of secreting 1,3-xylanases, designated as strain HB14T. Phylogenetic analysis revealed that strain HB14T clustered tightly with known species of the genus Gilvimarinus, showing the highest 16S rRNA gene sequence similarity (97.7%) with the type strain of Gilvimarinus chinensis. Based on phylogenetic, genomic, chemotaxonomic and phenotypic studies, strain HB14T was classified as a representative of a novel species in the genus Gilvimarinus, for which the name Gilvimarinus xylanilyticus sp. nov. was proposed. The type strain is HB14T (=CCTCC AB 2022109T = KCTC 92379T). Four 1,3-xylanases secreted by strain HB14T were identified based on genome and secretome analyses, and the two (Xyn65 and Xyn80) with relatively higher abundance in secretome were successfully expressed in Escherichia coli and biochemically characterized. They showed the highest activity at pH 6.0–7.0 and 40°C and released mainly 1,3-xylobiose and 1,3-xylotriose from 1,3-xylan. These data suggest that strain HB14T acts as a player in marine 1,3-xylan degradation and recycling and that its extracellular 1,3-xylanases may have a good potential in 1,3-xylooligosaccharides preparation.

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Section: Resultsmentioning

confidence: 99%

Section: Morphological Physiological and Biochemical Characteristicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gilvimarinus xylanilyticus sp. nov., a novel 1,3-xylanase-secreting bacterium isolated from a marine green alga

Zhang

Sun

et al. 2022

Front. Microbiol.

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“…Several agarose-degrading strains with special properties (e.g., cold adaptability) [24] have been isolated from the deep sea, such as Gilvimarinus agarilyticus JEA5 [25] and Shewanella sp. WPAGA9 [26].…”

Section: Introductionmentioning

confidence: 99%

Agarose biodegradation by deep‐sea bacterium Vibrio natriegens WPAGA4 with the agarases through horizontal gene transfer

Zhang,

Tong,

Wang

et al. 2023

J Basic Microbiol

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This study aimed to reveal the importance of horizontal gene transfer (HGT) for the agarose‐degrading ability and the related degradation pathway of a deep‐sea bacterium Vibrio natriegens WPAGA4, which was rarely reported in former works. A total of four agarases belonged to the GH50 family, including Aga3418, Aga3419, Aga3420, and Aga3472, were annotated and expressed in Escherichia coli cells. The agarose degradation products of Aga3418, Aga3420, and Aga3472 were neoagarobiose, while those of Aga3419 were neoagarobiose and neoagarotetraose. The RT‐qPCR analysis showed that the expression level ratio of Aga3418, Aga3419, Aga3420, and Aga3472 was stable at about 1:1:1.5:2.5 during the degradation, which indicated the optimal expression level ratio of the agarases for agarose degradation by V. natriegens WPAGA4. Based on the genomic information, three of four agarases and other agarose‐degrading related genes were in a genome island with a G + C content that was obviously lower than that of the whole genome of V. natriegens WPAGA4, indicating that these agarose‐degrading genes were required through HGT. Our results demonstrated that the expression level ratio instead of the expression level itself of agarase genes was crucial for agarose degradation by V. natriegens WPAGA4, and HGT occurred in the deep‐sea environment, thereby promoting the deep‐sea carbon cycle and providing a reference for studying the evolution and transfer pathways of agar‐related genes.

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“…Agarases are the enzyme groups that catalyze the depolymerization of agar and agarose into agaro and neoagaro oligosaccharides (AOS and NAOS) [2]. These oligosaccharide products parade their applications in the food, cosmetic, medical and pharmaceutical industries [3]. Amylases break down starch molecules' glycosidic linkages and they account for around 30% of the global enzyme market in the industrial sector [4].…”

Section: Introductionmentioning

confidence: 99%

Agarase, Amylase and Xylanase from Halomonas meridiana: A Study on Optimization of Coproduction for Biomass Saccharification

Veerakumar

Manian

2022

Fermentation

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Coproduction of multienzymes from single potential microbe has captivated contemplation in industries. Bacterial strain, Halomonas meridiana VITSVRP14, isolated from seaweed was labored to produce amylase, agarase and xylanase conjointly using submerged fermentation. The optimum production conditions clinched by classical optimization were: pH 8; 1.5% inoculum; 24 h incubation, 40 °C; 8% NaCl (sodium chloride); 1% lactose and NaNO3 (sodium nitrate). The preponderant variables (pH, temperature, lactose) and their interaction effect on enzyme production were studied by Plackett-Burman design and Response Surface Methodology (RSM). There were 3.29, 1.81 and 2.08 fold increase in enzyme activity with respect to agarase, amylase and xylanase after optimization against basal medium. After 24 h of enzymatic treatment, the saccharification rates of the coproduced enzyme mixture were 38.96% on rice bran, 49.85% on wheat bran, 61.2% on cassava bagasse and 57.82% on corn cob. Thus, the coproduced enzyme mixture from a bacterium with halotolerance is plausible in pretreated lignocellulose degradation. The ability of this single microbe Halomonas meridiana VITSVRP14, in coproducing agarase, amylase and xylanase give the nod for its application in biomass saccharification by subsiding cost, energy and time involved in the process.

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A Novel Agarase, Gaa16B, Isolated from the Marine Bacterium Gilvimarinus agarilyticus JEA5, and the Moisturizing Effect of Its Partial Hydrolysis Products

Cited by 9 publications

References 34 publications

Gilvimarinus xylanilyticus sp. nov., a novel 1,3-xylanase-secreting bacterium isolated from a marine green alga

Gilvimarinus xylanilyticus sp. nov., a novel 1,3-xylanase-secreting bacterium isolated from a marine green alga

Agarose biodegradation by deep‐sea bacterium Vibrio natriegens WPAGA4 with the agarases through horizontal gene transfer

Agarase, Amylase and Xylanase from Halomonas meridiana: A Study on Optimization of Coproduction for Biomass Saccharification

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