A novel amino acid substitution Trp574Arg     in acetolactate synthase (ALS) confers broad resistance to ALS‐inhibiting herbicides in crabgrass (<scp><i>Digitaria sanguinalis</i></scp>)

Li, Jian; Mei, Li; Gao, Xingxiang; Fěng, Fang

doi:10.1002/ps.4651

Cited by 32 publications

(24 citation statements)

References 30 publications

(60 reference statements)

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“…Trp 574 Arg, which was detected in only one plant of 17–56 H, had previously been found in Digitaria sanguinalis (L.) Scop. causing a broad‐spectrum resistance to nicosulfuron, imazethapyr and flumetsulam . Notably, survival rates to thifensulfuron‐methyl and imazamox of each cross‐resistant accession, i.e .…”

Section: Discussioncontrasting

confidence: 96%

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A family affair: resistance mechanism and alternative control of three Amaranthus species resistant to acetolactate synthase inhibitors in Italy

Milani

Scarabel

Sattin

2019

Pest Management Science

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BACKGROUND Several soybean fields in Italy were found to be infested by multiple species of Amaranthus spp. not adequately controlled by acetolactate (ALS) inhibitor herbicides. The objectives of this research were (i) to create a simplified botanical key to identify weedy amaranths; (ii) to determine the number and type of sites of action the accession are resistant to, i.e. resistance pattern; and (iii) to determine the main resistance mechanisms involved d) to evaluate the efficacy of herbicides with different site of action. RESULTS An easy‐to‐use botanical key was devised and successfully used in the infested sites and results were confirmed through a species‐specific molecular marker. Amaranthus retroflexus L. (redrood pigweed) was found in three sites; plants with Asp376Glu substitution at the ALS gene were resistant to thifensulfuron‐methyl. Amaranthus tuberculatus (Moq.) J.D.Sauer (waterhemp) and Amaranthus hybridus L. (smooth pigweed) accessions were cross‐resistant to thifensulfuron‐methyl and imazamox; most ALS‐resistant plants had a point mutation at position 574. One A. hybridus accession had the substitution Trp574Met, new for Amaranthus genus. All ALS‐resistant accessions were controlled by glyphosate and metribuzin. A. retroflexus accessions were controlled by bentazon, instead an A. hybridus and some A. tuberculatus accession were not. CONCLUSIONS The simplified botanical key proposed herein could be a useful tool for farmers and weed scientists to reliably identify Amaranthus species in the field. The main resistance mechanism in the three Amaranthus species is target‐site mediated. This is the first evidence of ALS‐resistant A. tuberculatus outside its native North American range. © 2019 Society of Chemical Industry

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Section: Discussioncontrasting

confidence: 96%

“…causing a broad-spectrum resistance to nicosulfuron, imazethapyr and flumetsulam. 51 Notably, survival rates to thifensulfuron-methyl and imazamox of each cross-resistant accession, i.e. A. hybridus and A. tuberculatus, had comparable values.…”

Section: Discussionmentioning

confidence: 91%

A family affair: resistance mechanism and alternative control of three Amaranthus species resistant to acetolactate synthase inhibitors in Italy

Milani

Scarabel

Sattin

2019

Pest Management Science

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“…Juncus effusus, treated with the ALS inhibiting herbicide Imazamox, an Imidazolinone class herbicide, a single mutation was identified in one of the six plants sampled. This is an active site mutation, W574R (Arabidopsis numbering), W548R (Juncus numbering) previously described in the weed Digitaria sanguinalis (Li et al 2017).…”

Section: Herbicide Target Genesmentioning

confidence: 99%

“…Though sequences were obtained for each isolate, a mutation was only discovered in Juncus effucus acetolactate synthase. This variant is cognate to the W574R (Arabidopsis numbering) mutation previously described in Digitaria sanguinalis (Li et al 2017). However, this is the first report of the sequence of this transcript and the first discovery of a herbicide inhibiting mutant in any Poales species.…”

Section: Herbicide Target Genesmentioning

confidence: 99%

Combining protein-based transcriptome assembly, and efficient MinION long read sequencing for targeted transcript sequencing in orphan species. Validation on herbicide targets and low copy number genes in Gymnosperms, Juncaceae and Pteridophyta

Evans

2020

Preprint

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Orphan species that are evolutionarily distant from their closest sequenced/assembled neighbour provide a significant challenge in terms of gene or transcript assembly for functional analysis. This is because 30% sequence divergence from the closest available reference sequence means that, even with a complete genome or transcriptome sequence, mapping-based or reference-based approaches to gene assembly and gene identification break down. A new approach is required for reference-guided gene and transcript assembly in such orphan species, or species that are evolutionarily very divergent from their closest relatives. When annotating genes, the protein sequence is often preferred as it diverges less than the DNA/RNA sequence and it is often simpler to find meaningful homology at the protein level. This greater conservation of protein sequence across evolutionary time also makes proteins a prime candidate for use as the basis for sequence assembly. A protein-based pipeline was developed for transcript assembly between distantly related species. This was tested on three evolutionarily divergent species with little sequence information available for them and for which the closest genome representatives were at least 40 million years divergent as well as one species (Azolla filiculoides) for which a genome assembly is available. All the species have the potential to be weeds and herbicide targets were chosen as functional genes, whilst low copy number genes were chosen for evolutionary studies. Transcriptomic sequences were assembled using a bait and assemble strategy and final assemblies were verified by direct sequencing.

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“…The Trp-574-Arg substitution was first reported in Digitaria sanguinalis in China [40]. Substitutions at the Trp-574 site have been documented to confer broad cross-resistance to the ALS-inhibiting chemistries.…”

Section: Summary By Herbicide Groupmentioning

confidence: 99%

Target-Site Mutations Conferring Herbicide Resistance

Murphy

Tranel

2019

Plants

107

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Mutations conferring evolved herbicide resistance in weeds are known in nine different herbicide sites of action. This review summarizes recently reported resistance-conferring mutations for each of these nine target sites. One emerging trend is an increase in reports of multiple mutations, including multiple amino acid changes at the glyphosate target site, as well as mutations involving two nucleotide changes at a single amino acid codon. Standard reference sequences are suggested for target sites for which standards do not already exist. We also discuss experimental approaches for investigating cross-resistance patterns and for investigating fitness costs of specific target-site mutations.

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A novel amino acid substitution Trp574Arg in acetolactate synthase (ALS) confers broad resistance to ALS‐inhibiting herbicides in crabgrass (Digitaria sanguinalis)

Cited by 32 publications

References 30 publications

A family affair: resistance mechanism and alternative control of three Amaranthus species resistant to acetolactate synthase inhibitors in Italy

A family affair: resistance mechanism and alternative control of three Amaranthus species resistant to acetolactate synthase inhibitors in Italy

Combining protein-based transcriptome assembly, and efficient MinION long read sequencing for targeted transcript sequencing in orphan species. Validation on herbicide targets and low copy number genes in Gymnosperms, Juncaceae and Pteridophyta

Target-Site Mutations Conferring Herbicide Resistance

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