A novel amperometric biosensor for the detection of nitrophenol

Kafi, A.K.M.; Chen, Aicheng

doi:10.1016/j.talanta.2009.03.015

Cited by 79 publications

(30 citation statements)

References 37 publications

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“…The catalytic current was linearly dependent on the 4-nitrophenol concentration within the 10-to 30-μM range with a linear correlation coefficient of 0.992. The limit of detection was 0.2 μM at a three times S/N ratio which is comparable to the 0.11 μM reported for HRP and methylene blue with chitosan immobilised on Au-modified TiO 2 nanotube arrays [68]. The repeatability (RDS) was determined for 10 μM 4-nitrophenol to be 5.5% for n=10 within one electrode and 8.5% between different electrodes for n=5.…”

Section: Peroxide-dependent Hydroxylation Of Aromatic Substratessupporting

confidence: 69%

“…The result for AaeAPO is in accordance with its remarkable ability to peroxygenate the aromatic rings of toluene and naphthalene to p-and o-cresol as well as naphthalene 1,2- [50] oxide and naphthols, respectively [37,48]. Kafi and Chen [68] reported that HRP immobilised with methylene blue in chitosan on Au-modified TiO 2 nanotubes oxidised 3-nitrophenol, 4-nitrophenol and five other phenolic compounds. The radical products generated from the phenolic substrates could be electrochemically reduced at the electrode at −50 mV, resulting in reduction peaks which were proportional to the substrate concentration.…”

Section: Peroxide-dependent Hydroxylation Of Aromatic Substratessupporting

confidence: 59%

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Can peroxygenase and microperoxidase substitute cytochrome P450 in biosensors

Yarman

Peng

et al. 2011

Bioanal Rev

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Aromatic peroxygenase (APO) from the basidiomycetous mushroom Agrocybe aegerita (AaeAPO) and microperoxidases (MPs) obtained from cytochrome c exhibit a broad substrate spectrum including hydroxylation of selected aromatic substrates, demethylation and epoxidation by means of hydrogen peroxide. It overlaps with that of cytochrome P450 (P450), making MPs and APOs to alternate recognition elements in biosensors for the detection of typical P450 substrates. Here, we discuss recently developed approaches using microperoxidases and peroxygenases in view of their potential to supplement P450 enzymes as recognition elements in biosensors for aromatic compounds. Starting as early as the 1970s, the direct electron transfer between electrodes and the heme group of heme peptides called microperoxidases has been used as a model of oxidoreductases. These MP-modified electrodes are used as hydrogen peroxide detectors based on the catalytic current generated by electrically contacted microperoxidase molecules. A similar catalytic reaction has been obtained for the electrode-immobilised heme protein AaeAPO. However, up to now, no MP-based sensors for substrates have been described. In this review, we present biosensors which indicate 4-nitrophenol, aniline, naphthalene and p-aminophenol based on the peroxide-dependent substrate conversion by electrode-immobilised MP and AaeAPO. In these enzyme electrodes, the signal is generated by the conversion of all substrates, thus representing in complex media an overall parameter. The performance of these sensors and their further development are discussed in comparison with P450-based electrodes

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Section: Peroxide-dependent Hydroxylation Of Aromatic Substratessupporting

confidence: 69%

Section: Peroxide-dependent Hydroxylation Of Aromatic Substratessupporting

confidence: 59%

Can peroxygenase and microperoxidase substitute cytochrome P450 in biosensors

Yarman

Peng

et al. 2011

Bioanal Rev

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“…The use of oxidative enzymes such as laccase [8], tyrosinase [9] and horseradish peroxidase (HRP) [10] to design electrochemical biosensors for detection of phenolic compounds has received great attention. However, reaction mechanisms of the biosensors based on tyrosinase, laccase and HRP are different for various types of phenolic compounds.…”

Section: Introductionmentioning

confidence: 99%

Laccase immobilization on the electrode surface to design a biosensor for the detection of phenolic compound such as catechol

Nazari

Kashanian

Rafipour

2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…Except for the electrochemical approach, all the other methods are either time consuming or require expensive facilities. Amperometric biosensors act as an important subclass of chemical sensors, in which an electrode is used as the transduction element, because they are highly qualified for meeting the cost, size, and short response time requirements in medical and environmental analysis [5,6]. Because of simplicity, high selectivity and high sensitivity, the amperometric system has been extensively employed towards designing the H 2 O 2 biosensor [7][8][9].…”

Section: Introductionmentioning

confidence: 99%