A Novel Amperometric Glucose Biosensor Based on Fe₃O₄‐Chitosan‐β‐Cyclodextrin/MWCNTs Nanobiocomposite

Abstract

A novel enzymatic biosensing platform toward glucose is achieved with nanocomposite of magnetic nanoparticles (Fe3O4−CS−CD) and multi‐walled carbon nanotubes (MWCNTs). The synergistic effect of chitosan, β‐cyclodextrin and MWCNTs can facilitate electron transfer between enzyme and electrode based on the promoting results of the cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The new biosensors exhibited direct electron transfer (DET) from enzyme to electrode after glucose oxidase (GOx) was immobilized on the modified electrode with the nanocomposite. Consequently, the enzymatic glucose biosensor displayed a considerably wide linear range (40 μM to 1.04 mM) with a high sensitivity of 23.59 μA mM−1cm−2, low detection limit of 19.30 μM, good selectivity, reproducibility and repeatability for detecting glucose. In addition, the current response still retained at 93.4 % after 25 days. Furthermore, the practical application of glucose biosensor was test in human serum samples with satisfactory accuracy, demonstrating promising and practical potential in biomedical diagnostics.

Electrochemical Immunosensor for Lactate Dehydrogenase Detection Through Analyte‐driven Catalytic Reaction on Multi‐walled Carbon Nanotubes and Gold Nanoparticle Modified Carbon Electrode

“…The corresponding linear equation was Y = 25.78807 + 4.50166X with a correlation coefficient (R 2 ) of 0.99186 (n = 3). The LOD calculated based on the 3σ b /slope criteria was 0.39 ng/mL, where σ b represented the standard deviation of the blank samples (n = 3) [21]. Admittedly, the proposed method, under consideration of LOD, performed better than that of previously reported methods (Table 1) [22][23][24][25][26][27].…”

Magnetically Stimulated Bio‐ and Electrochemical Systems: State‐of‐the‐Art, Applications, and Future Directions

“…Food safety, heavy ions, sugars dopamine detection [34,[47][48][49] Fe In MET-based bioelectrocatalysis, a redox mediator is required to transfer electrons between the oxidoreductase enzyme and the electrode surface. Adapted with permission, [58] Copyright 2020, Multidisciplinary Digital Publishing Institute (MDPI).…”

Immobilization of laccase on Fe3O4@MF-CNTs for the rapid and sensitive biosensing of catechol

“…An effective strategy to solve this defect is to utilize CNTs to enhance the electroconductivity of magnetic nanocomposites. 28 Herein, we synthesized an Fe 3 O 4 @MF-CNTs-Lac biosensor for the detection of catechol. The combination of Fe 3 O 4 @MF and CNTs can improve the conductivity of the Fe 3 O 4 @MF-CNT nanocomposites, which facilitates electron transfer between laccase and an electrode.…”

“…Fe 3 O 4 based magnetic nanoparticles are promising biomolecule carriers for biosensors owing to their biocompatibility, less toxicity and unique magnetic properties. 18 Since Fe 3 O 4 nanoparticles are highly susceptible to acidic and oxidative conditions, the catalytic activity of the immobilized laccase directly on the bare Fe 3 O 4 nanoparticles would be rapidly decreased. 19,20 Coating an outer protective layer is highly obligatory to maintain the stability of Fe 3 O 4 nanoparticles.…”

“…An effective strategy to solve this defect is to utilize CNTs to enhance the electroconductivity of magnetic nanocomposites. 28…”