A novel and effective procedure for removing HIV-1 RNA from human semen

A novel antibiotic cocktail for eliminating bacteria in human semen

“…1 indicated that a 15-min incubation, with a 1:1 (50%) dilution of the medium containing the antibiotic cocktail, was sufficient to eliminate all four microorganisms tested. Treatment of human semen with this novel antibiotic cocktail forms part of a combined protocol that includes a multiple density gradient centrifugation step that exposes sperm briefly to a proteolytic enzyme [7]. The combined treatment can eliminate a variety of microbes in semen without significantly affecting sperm vitality or functionality and, thus, has the potential to streamline and safeguard sperm processing in the future.…”

Affordable ART services in Africa: synthesis and adaptation of laboratory services

“…Figure 2) combined with a discontinuous density gradient containing a recombinant serine protease and inhibitor (PureSperm ® Pro Top and Bottom Layer, Nidacon), we have illustrated that HIV-1, HCV and CMV and white blood cells can be removed from spiked semen samples (Loskutoff et al, 2004(Loskutoff et al, , 2005Huyser et al, 2006). Recent (unpublished) results indicated that the insert also removed various bacteria concentrations efficiently when compared with traditional density gradient centrifugation.…”