A novel and rapid method to determine doxycycline in human plasma by liquid chromatography tandem mass spectrometry

Krishna, A Chaitanya; Sathiyaraj, M; Saravanan, R; Chelladurai, R; Vignesh, R.

doi:10.4103/0250-474x.110599

Cited by 14 publications

(6 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Food coatings from edible films may contain lipids, proteins, carbohydrates or their combinations and are reported to prolong food shelf-life and reduce the rate of hazardous occurrence in food products ( Guilbert, 1986 ; Kester and Fennema, 1986 ; Krishna et al ., 2012 ; Krochta and Johnston, 1997 ). From this point of view, the use of an edible film chitosan [poly-a-(1-4)-2-amino-2-deoxy-D-glucose], has been increasingly preferred in recent years.…”

Section: Introductionmentioning

confidence: 99%

Chitosan/whey Protein (CWP) Edible Films Efficiency for Controlling Mould Growth and on Microbiological, Chemical and Sensory Properties During Storage of Göbek Kashar Cheese

Yangılar¹

2015

Korean Journal for Food Science of Animal Resources

View full text Add to dashboard Cite

The objective of present study was to evaluate the effects of the application of chitosan and chitosan/whey protein on the chemical, microbial and organoleptic properties of Göbek Kashar cheese during ripening time (on 3rd, 30th, 60th and 90th d). Difference in microbiological and chemical changes between samples was found to be significant (p<0.05) during ripening period. Cheese samples with edible coating had statistically lower mould counts compared to the uncoated samples. Furthermore the highest and lowest mould counts were determined in control (4.20 Log CFU/g) and other samples (<1 Log CFU/g) at 60th and 90th d of storage. All samples exhibited higher levels of water soluble nitrogen and ripening index at the end of storage process. At the end of 90 day storage period, no signicant dierences in salt and fat values were observed among the cheeses studied. The edible coatings had a beneficial effect on the sensory quality of cheese samples. In the result of sensory analysis, while cheese C and the chitosan coated cheese samples were more preferred by the panellists, the chitosan/whey protein film-coated cheese samples received the lowest scores. This study shows coating suggests could be used to improve the quality of cheese during ripening time.

show abstract

Section: Introductionmentioning

confidence: 99%

Chitosan/whey Protein (CWP) Edible Films Efficiency for Controlling Mould Growth and on Microbiological, Chemical and Sensory Properties During Storage of Göbek Kashar Cheese

Yangılar¹

2015

Korean Journal for Food Science of Animal Resources

View full text Add to dashboard Cite

show abstract

“…MS were also found 6 . The use of a mobile phone instead of the UV spectrophotometer for the detection of the color intensity would be highly beneficial as well as cost-effective.…”

Section: Ijpsr (2020) Volume 11 Issue 11 (Research Article)mentioning

confidence: 90%

“…Thus the analysis of the uncertainty is needed in such cases. The simple recovery may be calculated by the help of equations (6) and 7shown below, depending on the spiked and the recovered concentration of the standard.…”

mentioning

confidence: 99%

Untitled

2020

IJPSR

View full text Add to dashboard Cite

“…Kromatografi partisi fase terbalik dengan kolom C 18 merupakan metode yang umum digunakan saat ini karena lebih sederhana dan selektif dengan waktu analisis yang lebih singkat (Harmita, 2006). Beberapa metode kromatografi cair dengan spektrometri massa (Krishna et al, 2012), UV-Vis (Mustariachie et al, 2011) …”

Section: Pendahuluanunclassified

Validasi Metode Analisis Ofloksasin dalam Plasma In Vitro secara Kromatografi Cair Kinerja Tinggi-Fluoresensi Mengacu pada European Medicines Agency Guideline

Tania¹,

Sitepu²,

Harahap³

2016

Pharm Sci Res

View full text Add to dashboard Cite

AbstrakOfloksasin merupakan salah satu antibiotika golongan fluorokuinolon generasi kedua. Konsentrasi ofloksasin dalam plasma kecil, sehingga diperlukan metode analisis yang selektif, akurat, dan sensitif. Pada penelitian ini, dilakukan optimasi dan validasi metode analisis ofloksasin dalam plasma in vitro menggunakan kromatografi cair kinerja tinggi deteksi fluoresensi dengan siprofloksasin HCl sebagai baku dalam. Pemisahan dilakukan dengan menggunakan kolom C 18 (Waters, Sunfire TM 5 µm; 250 x 4,6 mm) dengan fase gerak isokratik yang terdiri dari trietilamin 1% dalam air pH 3,0-asetonitril (84:16), laju alir 1,0 mL/menit, suhu kolom 40 o C, dan deteksi ofloksasin dilakukan pada panjang gelombang eksitasi 300 nm dan emisi 500 nm. Proses ekstraksi plasma dilakukan dengan metode pengendapan protein menggunakan metanol melalui proses vorteks selama 2 menit dan sentrifugasi pada kecepatan 10000 rpm selama 10 menit. Validitas dan linearitas metode analisis berada pada rentang konsentrasi 21,4 ng/mL -4280 ng/mL dengan LLOQ 21,4 ng/mL. Nilai % diff dan koefisien variasi untuk akurasi dan presisi intra hari dan antar hari tidak lebih dari + 20% untuk konsentrasi LLOQ dan + 15% untuk konsentrasi rendah, sedang, dan tinggi. Ofloksasin dalam plasma dinyatakan stabil selama 3 siklus beku dan cair, stabil minimal 24 jam pada suhu kamar dan selama minimal 28 hari pada suhu -20 o C. Metode analisis ofloksasin dalam plasma ini sudah memenuhi kriteria penerimaan sesuai persyaratan validasi pedoman EMEA. AbstractOfloxacin is an antibiotic from second generation of fluoroquinolones group. Concentration of ofloxacin in human plasma is in low level, and due to that fact, it requires a selective, accurate, sensitive method of analysis. In this study, the optimization and validation of ofloxacin analysis in human plasma using high performance liquid chromatography-fluorescence with ciprofloxacin-HCl as an internal standard were carried out. Separation of ofloxacin was performed using C 18 (Waters, Sunfire TM 5 µm; 250 x 4.6 mm) column with an isocratic mobile phase consisted of triethylamine 1% in water pH 3.0-acetonitrile (84:16) in the flow rate of 1.0 mL/min, 40 o C column temperature whereas the detection was carried out at excitation of 300 nm and emission of 500 nm. Plasma extraction was done by deproteination using methanol, through the process of vortex and centrifugation (10000 rpm) for 2 minutes and 10 minutes consecutively. The method was valid and linear within the concentration ranged from 21,4 ng/mL to 4280 ng/mL with LLOQ of 21,4 ng/mL. Intra-day and inter-day accuracy and presicion was not more than + 20% for LLOQ and not more than + 15% for QCL, QCM, and QCH samples in both % diff and coefficient of variation. Ofloxacin was stable in human plasma at least three freeze and thaw cycle, for at least 24 hours in room temperature and 28 days at -20 o C. This bioanalytical method fulfilled the acceptance criteria following EMEA guideline.

show abstract

A novel and rapid method to determine doxycycline in human plasma by liquid chromatography tandem mass spectrometry

Cited by 14 publications

References 9 publications

Chitosan/whey Protein (CWP) Edible Films Efficiency for Controlling Mould Growth and on Microbiological, Chemical and Sensory Properties During Storage of Göbek Kashar Cheese

Chitosan/whey Protein (CWP) Edible Films Efficiency for Controlling Mould Growth and on Microbiological, Chemical and Sensory Properties During Storage of Göbek Kashar Cheese

Untitled

Validasi Metode Analisis Ofloksasin dalam Plasma In Vitro secara Kromatografi Cair Kinerja Tinggi-Fluoresensi Mengacu pada European Medicines Agency Guideline

Contact Info

Product

Resources

About