A novel and simple biosensor based on poly(indoleacetic acid) film and its application for simultaneous electrochemical determination of dopamine and epinephrine in the presence of ascorbic acid

Abstract

A novel and simple biosensor based on poly (indoleacetic acid) film-modified electrode (PIAA/CPE) was fabricated by electrochemical polymerization of indoleacetic acid on a carbon paste electrode (CPE) through cyclic voltammetry. The resulting electrode was characterized by scanning electron microscopy, and the electrochemical behaviors of dopamine (DA) and epinephrine (EP) at the electrode were studied. It was illustrated that PIAA/CPE had excellent electrochemical catalytic activities toward DA and EP. The anodic peak currents (I pa ) were dramatically enhanced by about seven-fold for DA and ten times for EP at PIAA/CPE. Thus, the determinations of DA and EP were carried out using PIAA/CPE successfully. The linear responses were obtained in the range of 3.0×10 −7 ∼7.0× 10 −4 and 1.0×10 −6 ∼8.0×10 −4 mol L −1 with the detection limits (3σ) of 1×10 −7 and 4×10 −7 mol L −1 corresponding with DA and EP, respectively. Moreover, the cathodic peaks of DA and EP were well-separated with a potential difference about 325 mV in pH 5.3 phosphate-buffered saline, so simultaneous determination of DA and EP was carried out in this paper. Additionally, the interference studies showed that the PIAA/CPE exhibited excellent selectivity in the presence of ascorbic acid (AA). With good selectivity and sensitivity, the present method has been successfully applied to the determination of DA and EP in pharmaceutical samples.

Electrochemical characterization of poly(fuchsine acid) modified glassy carbon electrode and its application for simultaneous determination of ascorbic acid, epinephrine and uric acid

“…The detection limits (S/N = 3) were determined to be 0.009, 0.01 and 0.03 μmol L −1 for AA, EP, and UA, respectively. These were much lower than those previously reported for the simultaneous detection of AA, EP, and UA [15][16][17][18][19][20][21][22][23]. The anodic peak currents of AA, EP, and UA were reduced to 97.9%, 98.7% and 97.35% of the initial responses, respectively, after 40 consecutive CV measurements in the same condition.…”

Simultaneous determination of epinephrine and dopamine by electrochemical reduction on the hybrid material SiO2/graphene oxide decorated with Ag nanoparticles

“…Different methods have ben employed in the determination of catecholamines, including chromatography 22,23 , mass spectroscopy 24 , fluorescence 25 and electrochemical approaches 26,27 .…”

Polymer/Iron Oxide Nanoparticle Modified Glassy Carbon Electrodes for the Enhanced Detection of Epinephrine

“…Various methods, including liquid chromatography , spectrophotometry , flow injection analysis , chemiluminescence , capillary electrophoresis , and photokinetics have been reported for the determination of epinephrine. However, since epinephrine is easily oxidized, electrochemical methods constitute a quicker, cost‐effective, simple and more sensitive alternative, and have been amongst the most appealing and suitable techniques employed . Despite the advantages, the electrochemical determination of epinephrine can be a challenging task.…”

“…Additionally, epinephrine coexists in natural environments with other electroactive species such as ascorbic acid and uric acid, which oxidize at unmodified electrodes at almost the same potential, leading to poor selectivity and reproducibility . To overcome these limitations, the use of modified electrodes for monitoring epinephrine has been proposed .…”