A novel anti‐hypertensive peptide derived from ovalbumin induces nitric oxide‐mediated vasorelaxation in an isolated SHR mesenteric artery

Abstract

In this report, we deal with the isolation of a novel vasorelaxing peptide from a chymotryptic digest of ovalbumin and its vasorelaxing activities. This peptide is composed of ArgAla-Asp-His-Pro-Phe (RADHPF) in its sequence, corresponding to residues 359^364 of ovalbumin. This peptide (30^300 W WM) exerted a dose-dependent vasodilation in an isolated mesenteric artery from a spontaneously hypertensive rat which was preconstricted by phenylephrine, besides the relaxation being endothelium-dependent. It is noteworthy that the nitric oxide synthase inhibitor N G -nitro-L-arginine methyl ester inhibited this relaxation, implying involvement of nitric oxide in its mechanism of action. Following oral administration of RADHPF at a dose of 10 mg/kg, the systolic blood pressure in a spontaneously hypertensive rat was significantly lowered.z 1999 Federation of European Biochemical Societies.

Peptides: Production, bioactivity, functionality, and applications

“…It is noteworthy that NO is regarded as an important signaling molecule in vasodilation, neurotransmission, and host immune defense (Ahn et al, 2012) and peptides from whey protein (Ballard et al, 2009), Aa-lactabumin and Ab-lactabumin (Sipola et al, 2002), ovalbumin (Matoba et al, 1999), flaxseed protein (Udenigwe and Aluko, 2012), skeletal muscle protein (Takahashi et al, 2009), and even human casein (Fujita et al, 1996) were found to enhance NO production.…”

β-Conglycinin Peptides Improve Glucose Uptake through the AMPK Signaling Pathway in L6 Myotubes

“…A number of bioactive peptides derived from the enzymatic digestion of food proteins exhibit various physiological functions, including antihypertensive (Matoba et al, 1999;Zhao, et al, 2008) and cholesterol-lowering (Yamauchi, et al, 2003) activities. et al (2011) showed that soymorphin-5, a pentapeptide derived from the β-subunit of βCG, improves glucose and lipid metabolism in KK-A y mice, probably via activation of the adiponectin and PPARγ pathways, followed by increases in β-oxidation and energy expenditure.…”

Functional Proteins and Peptides of Hen’s Egg Origin

“…A vasorelaxing peptide -ovokinin (2-7) -was isolated from chymotryptic digest of ovalbumin [87]. However, the mechanisms for the relaxation were different from ovokinin.…”