A novel anti-plant viral protein from coelomic fluid of the earthworm Eisenia foetida: Purification, characterization and its identification as a serine protease

Ueda, Mitsuhiro; Noda, Kanako; Nakazawa, Masami; Miyatake, Kazutaka; Ohki, Satoshi T.; Sakaguchi, Minoru; Inouye, Kuniyo

doi:10.1016/j.cbpb.2008.08.005

Cited by 23 publications

(12 citation statements)

References 19 publications

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“…In 2008, another serine protease was purified from the coelomic fluid of the earthworm E. fetida [27]. It has strong antiviral activities against cucumber mosaic virus and tomato mosaic virus.…”

Section: Isozymes From Eisenia Fetidamentioning

confidence: 99%

Earthworm Protease

Pan

Zhang

2010

Applied and Environmental Soil Science

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The alimentary tract of earthworm secretes a group of proteases with a relative wide substrate specificity. In 1983, six isozymes were isolated from earthworm with fibrinolytic activities and called fibriniolytic enzymes. So far, more isozymes have been found from different earthworm species such as Lumbricus rubellus and Eisenia fetida. For convenience, the proteases are named on the basis of the earthworm species and the protein function, for instance, Eisenia fetida protease (Ef P). The proteases have the abilities not only to hydrolyze fibrin and other protein, but also activate proenzymes such as plasminogen and prothrombin. In the light of recent studies, eight of the Ef Ps contain oligosaccharides chains which are thought to support the enzyme structure. Interestingly, Ef P-II has a broader substrate specificity presenting alkaline trypsin, chymotrypsin and elastase activities, but Ef P-III-1 has a stricter specificity. The protein crystal structures show the characteristics in their specificities. Earthworm proteases have been applied in several areas such as clinical treatment of clotting diseases, anti-tumor study, environmental protection and nutritional production. The current clinical utilizations and some potential new applications of the earthworm protease will be discussed in this paper.

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Section: Isozymes From Eisenia Fetidamentioning

confidence: 99%

Earthworm Protease

Pan

Zhang

2010

Applied and Environmental Soil Science

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“…SDS-PAGE was run with 12% acrylamide concentration and each fraction was loaded with standard low range protein ladder. Fractions having average molecular weight of the range [15][16][17][18][19][20] kDa and caseinolytic activity were pooled up and subjected to charge based separation [18] . DEAE-Cellulose (G E Healthcare product), an anion resin, was used for separation of protein on charge basis.…”

Section: Isolation and Purification Of Serine Proteasementioning

confidence: 99%

“…We have collected 20 fractions with a speed of 3mL per minute. Each fraction was assayed for caseinolytic activity and total protein content [19] .…”

Section: Isolation and Purification Of Serine Proteasementioning

confidence: 99%

“…All Sephadex-G50 fractions: 25 were analyzed for caseinolytic activity and SDS-PAGE analysis. Fraction number 18,19 and 20 were pooled and further purified with DEAE-Cellulose resin. Fraction number 11 and 12 were pooled up and subjected to further activity analysis.…”

Section: Isolation and Purification Of Serine Proteasementioning

confidence: 99%

“…Fraction number 11 and 12 were pooled up and subjected to further activity analysis. Basic criteria for selection of fractions in between molecular weight [15][16][17][18][19][20] kDa is based on the experimental study carried out using E. foetida [24] .…”

Section: Isolation and Purification Of Serine Proteasementioning

confidence: 99%

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