A novel approach for preventing esophageal stricture formation: olmesartan prevented apoptosis

Dereli, Murat Levent; Krazinski, Bartlomiej E.; Ayvaz, Süleyman; Aksu, Burhan; Kanter, Mehmet; Uzun, Hafize; Gelışgen, Remise; Umit, C. Hasan; İnan, Mustafa; Başaran, Ümit Nusret; Pul, Mehmet

doi:10.5603/fhc.2014.0003

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…Local injection of corticosteroids is another approach to prevent stricture formation which appears to be effective, but is associated with an increased risk of oesophageal perforation [12]. Similar to scars [13], oesophageal strictures [14] are characterized by an abnormal increase in the production and deposition of collagen. Corticosteroids have also been used to prevent scar‐formation in the skin [15], with one mode‐of‐action being reduction of fibroblast proliferation, the major collagen‐synthesizing cell [16].…”

Section: Introductionmentioning

confidence: 99%

Exosomes derived from clinical‐grade oral mucosal epithelial cell sheets promote wound healing

Sjöqvist

Ishikawa

Shimura

et al. 2019

J of Extracellular Vesicle

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The oral mucosa exhibits unique regenerative properties, sometimes referred to as foetal-like wound healing. Researchers from our institute have used sheets of oral mucosa epithelial cells (OMECs) for regenerative medicine applications including cornea replacement and oesophageal epithelial regeneration for stricture prevention. Here, we have isolated exosomes from clinical-grade production of OMEC sheets from healthy human donors (n = 8), aiming to evaluate the clinical potential of the exosomes to stimulate epithelial regeneration and to improve understanding of the mode-of-action of the cells. Exosomes were isolated from conditioned (cExo) and non-conditioned (ncExo) media. Characterization was performed using Western blot for common exosomal-markers: CD9 and flotillin were positive while annexin V, EpCam and contaminating marker GRP94 were negative. Nanoparticle tracking analysis revealed a diameter of ~120 nm and transmission electron microscopy showed a corresponding size and spherical appearance. Human skin fibroblasts exposed to exosomes showed dose-dependent reduction of proliferation and a considerable increase of growth factor gene expression (HGF, VEGFA, FGF2 and CTGF). The results were similar for both groups, but with a trend towards a larger effect from cExo. To study adhesion, fluorescently labelled exosomes were topically applied to pig oesophageal wound-beds ex vivo and subsequently washed. Positive signal could be detected after as little as 1 min of adhesion, but increased adhesion time produced a stronger signal. Next, labelled exosomes were added to full-thickness skin wounds in rats and signal was detected up to 5 days after application. cExo significantly reduced the wound size at days 6 and 17. In conclusion, exosomes from OMEC sheets showed pro-regenerative effects on skin wound healing. This is the first time that the healing capacity of the oral mucosa is studied from an exosome perspective. These findings might lead to a combinational therapy of cell sheets and exosomes for future patients with early oesophageal cancer.

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Section: Introductionmentioning

confidence: 99%

Exosomes derived from clinical‐grade oral mucosal epithelial cell sheets promote wound healing

Sjöqvist

Ishikawa

Shimura

et al. 2019

J of Extracellular Vesicle

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Caustic Esophageal Injury

French¹,

Sundaresan²

2019

Shackelford's Surgery of the Alimentary Tract, 2 Volume Set

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Small Extracellular Vesicles and Oral Mucosa: The Power Couple in Regenerative Therapies?

Borowiec,

Dyszkiewicz-Konwińska,

Bukowska

et al. 2024

Cells

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Although ongoing debates persist over the scope of phenomena classified as regenerative processes, the most up-to-date definition of regeneration is the replacement or restoration of damaged or missing cells, tissues, organs, or body parts to full functionality. Despite extensive research on this topic, new methods in regenerative medicine are continually sought, and existing ones are being improved. Small extracellular vesicles (sEVs) have gained attention for their regenerative potential, as evidenced by existing studies conducted by independent research groups. Of particular interest are sEVs derived from the oral mucosa, a tissue renowned for its rapid regeneration and minimal scarring. While the individual regenerative potential of both sEVs and the oral mucosa is somewhat understood, the combined potential of sEVs derived from the oral mucosa has not been sufficiently explored and highlighted in the existing literature. Serving as a broad compendium, it aims to provide scientists with essential and detailed information on this subject, including the nature of the materials employed, isolation and analysis methodologies, and clinical applications. The content of this survey aims to facilitate the comparison of diverse methods for working with sEVs derived from the oral mucosa, aiding in the planning of research endeavors and identifying potential research gaps.

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A novel approach for preventing esophageal stricture formation: olmesartan prevented apoptosis

Cited by 3 publications

References 36 publications

Exosomes derived from clinical‐grade oral mucosal epithelial cell sheets promote wound healing

Exosomes derived from clinical‐grade oral mucosal epithelial cell sheets promote wound healing

Caustic Esophageal Injury

Small Extracellular Vesicles and Oral Mucosa: The Power Couple in Regenerative Therapies?

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