A Novel Approach for Real-Time Enumeration of Escherichia coli ATCC 47076 in Water through High Multi-Functional Engineered Nano-Dispersible Electrode

Abstract

The climate change is significantly evolving novel microbes in the environment. In addition, nanoscience is advancing promptly to provide environmentally friendly engineering solutions to detect these microbes (i.e., pathogenic bacteria and viruses) in blood and water. There is a need to develop smart and efficient nano-biosensor to detect the pathogens, Escherichia coli (ATCC 47076) in the drinking water to protect the public against the diseases like hemolytic uremic, gastroenteritis, and acute diarrheas. The immunomagnetic separation strategy enables detecting bacteria in water samples fast and efficiently. The developed sensor is capable for the detection targeted E. coli ATCC 46076 based on Stripping differential pulse voltammetry (SDPV) and Cyclic Voltammetry (CV) measurements with a dynamic linear range of 101 to 107 CFU ml−1. Functionalized magnetite metal-organic frameworks (MOFs) serve as a capture probe and Spectro-electrochemical label. The developed disposable electrode offers advantages such as large dynamic range, high sensitivity, high selectivity, and short analysis time (5 min). As for as we know, this is the first report to display the potential of the AuNPs and MOFs nanoparticles based dispersible electrode for the detection of targeted E. coli from water and blood.

Recent advances in bioelectrochemical detection of E. coli bacteria in saline buffers and spiked real samples using traditional and nanostructured active substrates

“…At the same time, biocompatible polymeric films played an important role in coating MNPs by improving the surface functionality for antibody conjugation and aqueous stability. Three electrochemical immunosensing schemes are here proposed for discussions [50,51,52] (Figure 5, Table 2).…”

“…SPCEs were drop-cast with a mixture of L-lysine magnetic nanoparticles (MNP) exposed to glutaraldehyde/capture primary antibody/different suspensions of E. coli CECT 425 in PBS/a secondary horseradish peroxidase (HP)-modified antibody prepared in approximately 3 h and held with a magnet. The immunorecognition complexes were monitored by constant potential amperometry (CPA) in the presence of F I G U R E 5 Electrochemical immunodetection of E.coli strains using magnetic nanoparticles (MNP) on solid supports: (A) conductivity and impedance [50], (B) constant potential amperometry [51] and (C) stripping differential pulse voltammetry (SDPV) [52].…”

Water quality analysis of old and new Phuleli Canal for irrigation purpose in the vicinity of Hyderabad, Pakistan

“…Coliforms are the bacteria or organisms that present in environment (Panhwar et al 2021a(Panhwar et al , 2021b. In biological characteristics the Fecal Coliforms were targeted.…”

Biological Indicators for Fecal Pollution Detection and Source Tracking: A Review

“…These non-pathogenic strains are considered safe fecal indicators (categorized as biosafety Level I organisms in the U.S.) and can be enumerated by similar methods, as described for coliforms in environmental waters. In addition, there are specialized methods that only detect certain E. coli strains, such as ATCC 47,076, in drinking water [109]. Pathogenic E. coli strains, including the aforementioned O157, a Shiga toxin-producing E. coli (STEC) serotype categorized as a biosafety Level II organism, caused 5441 infections across the United States in 1996 [110].…”