A novel approach to create an antibacterial surface using titanium dioxide and a combination of dip-pen nanolithography and soft lithography

Arango-Santander, Santiago; Peláez-Vargas, Alejandro; Freitas, Sidónio C.; García, Cristhian

doi:10.1038/s41598-018-34198-w

Cited by 39 publications

(32 citation statements)

References 54 publications

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“…In this regard, different metallic nanoparticles (NPs) including silver (Ag) [4][5][6], gold (Au) [7], zinc (Zn) [8], selenium (Se) [9,10], titanium [11], ceria [12] have been explored [13]. Se is an essential metalloid element that has been extensively used in medical applications owing to its antioxidative, antimicrobial and antitumor activities [14].…”

Section: Introductionmentioning

confidence: 99%

Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

et al. 2020

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The exploitation of plant extracts for the synthesis of nano selenium having antibacterial and antioxidant activities is an exciting approach to counteract the prevalence of infections caused by antibiotic-resistant bacteria, which holds relevance for medical and food industries. In the present work, a green and facile method for the preparation of nano selenium (nSe) using the fruit extract of Indian gooseberry (Phyllanthus Emblica) has been reported. The optical and structural properties of the as-synthesized nSe were studied through various characterization techniques. Eventually, the antioxidant potential of nSe was investigated via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl free radical scavenging assays. Parallely, the antibacterial activity of nSe against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa was evaluated. The antioxidant assays indicated that even low dosage of nSe showed excellent activity with EC 50 values of 0.21 μg ml −1 and 3.34 μg ml −1 , respectively. Moreover, nSe exhibited significant inhibition in bacterial growth at low minimum inhibitory concentration (MIC) values against Escherichia coli (16 μg ml −1 ), Staphylococcus aureus (32 μg ml −1 ) and Pseudomonas aeruginosa (48 μg ml −1 ) compared to MIC values for standard drug ampicillin. Importantly, nSe did not induce any cytotoxic effects on normal human keratinocytes (HaCaT) at the tested concentrations; representing their biocompatible nature. The data obtained demonstrated the versatility of phytogreen nSe as a potent antioxidant and antibacterial agent to effectively prevent as well as treat multidrug-resistant bacterial infections.

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Section: Introductionmentioning

confidence: 99%

Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

et al. 2020

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“…0.017 × 0.025 in, 10 mm-long 316L stainless steel and NiTi orthodontic archwire fragments (ORMCO, Orange, CA, USA) were used. The archwire fragments of the aforementioned dimensions were sequentially cleaned, following a protocol established by our group [12,35], using surfactant, acetone (99.8% v/v, Merck Millipore, Burlington, MA, USA), distilled water (Protokimica, Medellin, Colombia), and absolute ethanol (99% v/v, Merck Millipore, Burlington, MA, USA) for 8 min each in an ultrasound bath and let dry in air.…”

Section: Substratementioning

confidence: 99%

Assessment of Streptococcus Mutans Adhesion to the Surface of Biomimetically-Modified Orthodontic Archwires

et al. 2020

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Bacterial adhesion and biofilm formation on the surfaces of dental and orthodontic biomaterials is primary responsible for oral diseases and biomaterial deterioration. A number of alternatives to reduce bacterial adhesion to biomaterials, including surface modification using a variety of techniques, has been proposed. Even though surface modification has demonstrated a reduction in bacterial adhesion, information on surface modification and biomimetics to reduce bacterial adhesion to a surface is scarce. Therefore, the main objective of this work was to assess bacterial adhesion to orthodontic archwires that were modified following a biomimetic approach. The sample consisted of 0.017 × 0.025, 10 mm-long 316L stainless steel and NiTi orthodontic archwire fragments. For soft lithography, a polydimethylsiloxane (PDMS) stamp was obtained after duplicating the surface of Colocasia esculenta (L) Schott leaves. Topography transfer to the archwires was performed using silica sol. Surface hydrophobicity was assessed by contact angle and surface roughness by atomic force microscopy. Bacterial adhesion was evaluated using Streptococcus mutans. The topography of the Colocasia esculenta (L) Schott leaf was successfully transferred to the surface of the archwires. Contact angle and roughness between modified and unmodified archwire surfaces was statistically significant. A statistically significant reduction in Streptococcus mutans adhesion to modified archwires was also observed.

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“…Gradients can also be used to introduce a controlled degree of complexity into a biomaterial and, in turn, guide organism or cell behavior . Even more direct structuring on the nanolevel can be realized applying approaches such as dip‐pen lithography or AFM‐based oxidation techniques …”

Section: Arena Fabricationmentioning

confidence: 99%

Artificial Microbial Arenas: Materials for Observing and Manipulating Microbial Consortia

et al. 2019

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From the smallest ecological niche to global scale, communities of microbial life present a major factor in system regulation and stability. As long as laboratory studies remain restricted to single or few species assemblies, however, very little is known about the interaction patterns and exogenous factors controlling the dynamics of natural microbial communities. In combination with microfluidic technologies, progress in the manufacture of functional and stimuli-responsive materials makes artificial microbial arenas accessible. As habitats for natural or multispecies synthetic consortia, they are expected to not only enable detailed investigations, but also the training and the directed evolution of microbial communities in states of balance and disturbance, or under the effects of modulated stimuli and spontaneous response triggers. Here, a perspective on how materials research will play an essential role in generating answers to the most pertinent questions of microbial engineering is presented, and the concept of adaptive microbial arenas and possibilities for their construction from particulate microniches to 3D habitats is introduced. Materials as active and tunable components at the interface of living and nonliving matter offer exciting opportunities in this field. Beyond forming the physical horizon for microbial cultivates, they will enable dedicated intervention, training, and observation of microbial consortia. Microbe ManipulationThe ORCID identification number(s) for the author(s) of this article can be found under https://doi.

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A novel approach to create an antibacterial surface using titanium dioxide and a combination of dip-pen nanolithography and soft lithography

Cited by 39 publications

References 54 publications

Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

Assessment of Streptococcus Mutans Adhesion to the Surface of Biomimetically-Modified Orthodontic Archwires

Artificial Microbial Arenas: Materials for Observing and Manipulating Microbial Consortia

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