A novel approach to eliminateWolbachiainfections inNasonia vitripennisrevealed different antibiotic resistance between two bacterial strains

Abstract

Wolbachia are widespread in insects and can manipulate host reproduction. Nasonia vitripennis is a widely studied organism with a very high prevalence of Wolbachia infection. To study the effect of Wolbachia infection in Nasonia spp., it is important to obtain noninfected individuals by artificial methods. Current methods that employ sugar water-containing antibiotics can successfully eliminate Wolbachia from the parasitic wasps; however, treatment of at least three generations is required. Here, we describe a novel, feasible, and effective approach to eliminate Wolbachia from N. vitripennis by feeding fly pupae continuously offering antibiotics to Nasonia populations, which shortened the time to eliminate the pathogens to two generations. Additionally, the Wolbachia Uni and CauB strains have obviously different rifampicin-resistance abilities, which is a previously unknown phenomenon.

Bacteriophage WO Can Mediate Horizontal Gene Transfer in Endosymbiotic Wolbachia Genomes

“…The N. vitripennis populations were the Hangzhou strain (from the Gongyin Ye lab, ZheJiang University) (Zhang et al, 2005) infected with Wolbachia supergroup A (Liu et al, 2014). The housefly larvae were fed bran for 5–6 days until pupation.…”

Effects of Sublethal Concentrations of Tetracycline Hydrochloride on the Biological Characteristics and Wolbachia Titer in Parthenogenesis Trichogramma pretiosum

“…Previous studies showed that Wolbachia could be removed by feeding insects sugar water containing antibiotics, but the complete elimination of Wolbachia required at least three to six successive generations of antibiotic treatment [ 39 , 40 , 41 ]. Liu et al [ 42 ] described a novel approach to eliminate Wolbachia from Nasonia vitripennis by feeding pupae with rifampicin to two generations. Therefore, different antibiotics and methods could be used to try to eliminate Wolbachia from T. pretiosum in further research.…”

First report onWolbachiaendosymbiosis in freshwaterAphelocheirus aestivalis(Heteroptera: Aphelocheiridae) and its potential impact on genetic diversity of host

“…The similar approach was used in previous studies of Wolbachia diversity in parasitic wasp Nasonia vitripennis (Liu et al . ) or in terrestrial heteropteran bugs (Kikuchi & Fukatsu ). Here, we found that Wolbachia infecting A. aestivalis belongs to supergroup A.…”