A novel approach to the simultaneous extraction and non-targeted analysis of the small molecules metabolome and lipidome using 96-well solid phase extraction plates with column-switching technology

Li, Yubo; Zhang, Zhenzhu; Liu, Xinyu; Li, Aizhu; Hou, Zhiguo; Wang, Yuming; Zhang, Yanjun

doi:10.1016/j.chroma.2015.07.048

Cited by 31 publications

(18 citation statements)

References 20 publications

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“…A wide range of applications of this technique includes analysis of low‐molecular mass pharmaceuticals in clinical samples; detection of toxicological substances, illicit and medicinal drugs and their metabolites in human blood and urine; and quantification of natural biologically active compounds . There are few reports on the peptides extraction from biological matrixes by microelution plates, nevertheless it was proved to be useful for proteomic studies and pharmaceutical profiling of peptide drug …”

Section: Introductionmentioning

confidence: 99%

Solid‐phase extraction of small biologically active peptides on cartridges and microelution 96‐well plates from human urine

Semenistaya¹,

Zvereva²,

Krotov³

et al. 2015

Drug Testing and Analysis

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Currently liquid chromatography - mass spectrometry (LC-MS) analysis after solid-phase extraction (SPE) on weak cation-exchange cartridges is a method of choice for anti-doping analysis of small bioactive peptides such as growth hormone releasing peptides (GHRPs), desmoporessin, LHRH, and TB-500 short fragment. Dilution of urine samples with phosphate buffer for pH adjustment and SPE on weak cation exchange microelution plates was tested as a means to increase throughput of this analysis. Dilution using 200 mM phosphate buffer provides good buffering capacity without affecting the peptides recoveries. SPE on microelution plates was performed on Waters Positive Pressure-96 Processor with subsequent evaporation of eluates in nitrogen flow. Though the use of smaller sample volume decreases the pre-concentration factor and increases the limits of detection of 5 out of 17 detected peptides, the recovery, linearity, and reproducibility of the microelution extraction were comparable with cartridge SPE. The effectiveness of protocols was confirmed by analysis of urine samples containing ipamorelin, and GHRP-6 and its metabolites. SPE after urine sample dilution with buffer can be used for faster sample preparation. The use of microelution plates decreases consumption of solvents and allows processing of up to 96 samples simultaneously. Cartridge SPE with manual рН adjustment remains the best option for confirmation. Copyright © 2015 John Wiley & Sons, Ltd.

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Section: Introductionmentioning

confidence: 99%

Solid‐phase extraction of small biologically active peptides on cartridges and microelution 96‐well plates from human urine

Semenistaya¹,

Zvereva²,

Krotov³

et al. 2015

Drug Testing and Analysis

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“…These observations may be due to the larger surface area (230 m 2 /g) of the of the HSS‐T3 particles as compared with the others (185 m 2 /g for BEH and CSH, and 225 m 2 /g for Ascentis Express), which enables faster interactions of the analytes with the stationary phase, resulting in better retentivity (Ali et al, ; Wyndham et al, ). Additionally, the T3‐endcapping and lower ligand density of this column has been shown to perform well with low molecular weight, polar analytes (Gros et al, ; Li et al, ). The HSS‐T3 column was used in subsequent analyses.…”

Section: Resultsmentioning

confidence: 99%

Development of a Rapid Ultra High‐Performance Liquid Chromatography/Tandem Mass Spectrometry Method for the Analysis of sn‐1 and sn‐2 Lysophosphatidic Acid Regioisomers in Mouse Plasma

Aristizabal‐Henao

Fernandes

Duncan

2019

Lipids

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Lysophosphatidic acids (lysoPtdOH) are involved in several physiological processes including cell proliferation, inflammation, and glucose metabolism. However, measuring lysoPtdOH is challenging due to inadequate extraction techniques, poor chromatographic resolution, or the inability to discriminate between sn‐1 and sn‐2 regioisomers. In the present work, we developed a high‐throughput (10 min run times) ultra‐high‐performance liquid chromatography–tandem mass spectrometry method capable of discriminating lysoPtdOH species by their fatty acyl composition and sn‐localization on glycerol backbones. We quantitated sn‐1/sn‐2 regioisomeric pairs of lysoPtdOH with 16:0, 18:0, 18:1, 18:2, 20:4, and 22:6 fatty acyl chains using 50 μL of mouse plasma. The method presented here can be expanded to profile more lysoPtdOH species, and has the potential to be used in clinical settings to quickly screen lysoPtdOH profiles. Finally, the ability to discriminate between sn‐1 and sn‐2 isomers can provide insights regarding the metabolic origins and fates of specific lysoPtdOH molecules.

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“…C8 and C18 cartridges have been used to isolate cerebrosides, gangliosides, eicosanoids, steroid hormones and FAs from polar matrixes in biofluid samples [26,[29][30][31]. 96-well SPE plates enable pretreatment of 96 samples at once, which enables improved automation, throughput and parallel mode of lipid extractions [32,33]. The development of automated SPE that is coupled online with LC largely simplifies lipid extraction procedures [34][35][36].…”

Section: Universal Extraction Methodsmentioning

confidence: 99%

Mass‐spectrometry‐based lipidomics

Zhang

2017

J of Separation Science

128

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Lipids, which have a core function in energy storage, signalling and biofilm structures, play important roles in a variety of cellular processes because of the great diversity of their structural and physiochemical properties. Lipidomics is the large-scale profiling and quantification of biogenic lipid molecules, the comprehensive study of their pathways and the interpretation of their physiological significance based on analytical chemistry and statistical analysis. Lipidomics will not only provide insight into the physiological functions of lipid molecules but will also provide an approach to discovering important biomarkers for diagnosis or treatment of human diseases. Massspectrometry-based analytical techniques are currently the most widely used and most effective tools for lipid profiling and quantification. In this review, the field of massspectrometry-based lipidomics was discussed. Recent progress in all essential steps in lipidomics was carefully discussed in this review, including lipid extraction strategies, separation techniques and mass-spectrometry-based analytical and quantitative methods in lipidomics. We also focused on novel resolution strategies for difficult problems in determining C=C bond positions in lipidomics. Finally, new technologies that were developed in recent years including single-cell lipidomics, flux-based lipidomics and multiomics technologies were also reviewed.

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A novel approach to the simultaneous extraction and non-targeted analysis of the small molecules metabolome and lipidome using 96-well solid phase extraction plates with column-switching technology

Cited by 31 publications

References 20 publications

Solid‐phase extraction of small biologically active peptides on cartridges and microelution 96‐well plates from human urine

Solid‐phase extraction of small biologically active peptides on cartridges and microelution 96‐well plates from human urine

Development of a Rapid Ultra High‐Performance Liquid Chromatography/Tandem Mass Spectrometry Method for the Analysis of sn‐1 and sn‐2 Lysophosphatidic Acid Regioisomers in Mouse Plasma

Mass‐spectrometry‐based lipidomics

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