A novel aptasensor based on Fe3O4/Au/g-C3N4 for sensitive detection of sulfameter in food matrices

Yan, Xueling; Wang, Yarong; Kou, Qiming; Sun, Qi; Jian, Tang; Yang, Lulan; Chen, Xingyue; Xu, Wen; Le, Tao

doi:10.1016/j.snb.2021.131148

Cited by 19 publications

(16 citation statements)

References 21 publications

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“…Later, 0.15 g Au/g-C 3 N 4 , 0.5 mmol FeCl 3 ·6H 2 O, and 0.73 mmol of FeSO 4 ·7H 2 O were added to 50 mL DI H 2 O, followed by sonication for 1 h. The mixture was then transferred to a hydrothermal reactor, 3 mmol NaOH was added, and the mixture was then heated at 120 °C for 24 h. After cooling, the mixture was washed 4 times with DI H 2 O followed by ethanol. Finally, we obtained Fe 3 O 4 /Au/g-C 3 N 4 using magnetic separation and freeze-drying [ 12 , 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…All samples were purchased from the local supermarket and processed using a previously established procedure with slight modifications [ 12 ]. To prepare the milk samples, 2 mL of skimmed milk was centrifuged at 14,000 rpm at 4 °C for 20 min and diluted to 20 mL.…”

Section: Methodsmentioning

confidence: 99%

“…Aptamers are 70–100 nt RNA or ssDNA that are generally prepared through systematic evolution of ligands by exponential enrichment (SELEX) and often used as bioprobes, which recognize and bind targets with high affinity and specificity [ 11 ]. Aptamers can fold into unique spatial structures and can specifically bind to target molecules, such as cells, proteins, antibiotics, toxins, and other biomarkers, through hydrogen bonds, hydrophobic interactions, and van der Waals forces [ 12 ]. Kou screened the shortest aptamer (SMZ1S), which can be used as a capture probe to identify and combine SMZ to detect SMZ in the sample matrix.…”

Section: Introductionmentioning

confidence: 99%

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High-Sensitive FAM Labeled Aptasensor Based on Fe3O4/Au/g-C3N4 for the Detection of Sulfamethazine in Food Matrix

et al. 2022

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In this study, we developed a fluorescent aptasensor based on Fe3O4/Au/g-C3N4 and a FAM-labeled aptamer (FAM-SMZ1S) against sulfamethazine (SMZ) for the specific and sensitive detection of SMZ in food matrix. The FAM-SMZ1S was adsorbed by the Fe3O4/Au/g-C3N4 via π–π stacking and electrostatic adsorption, serving as a basis for the ultrasensitive detection of SMZ. Molecular dynamics was used to explain the reasons why SMZ1S and SMZ were combined. This aptasensor presented sensitive recognition performance, with a limit of detection of 0.16 ng/mL and a linear range of 1–100 ng/mL. The recovery rate ranged from 91.6% to 106.8%, and the coefficient of variation (CV) ranged from 2.8% to 13.4%. In addition, we tested the aptasensor for the monitoring of SMZ in various matrix samples, and the results were well-correlated (R2 ≥ 0.9153) with those obtained for HPLC detection. According to these results, the aptasensor was sensitive and accurate, representing a potentially useful tool for the detection of SMZ in food matrix.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High-Sensitive FAM Labeled Aptasensor Based on Fe3O4/Au/g-C3N4 for the Detection of Sulfamethazine in Food Matrix

et al. 2022

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“…A negative control without SQX was also included to assess nonspecific binding. The dissociation constant (K d ) value was obtained by the nonlinear fitting of Origin 9.0 software (Northampton, MA, USA) and calculated according to the nonlinear regression equation Y = B max × X/(K d + X) [28]; where X represents the concentration of aptamer, Y represents the relative fluorescence intensity, and B max represents the most binding sites. The smaller the K d value, the higher the affinity.…”

Section: Fluorescence Polarization-based Binding Affinity and Specifi...mentioning

confidence: 99%

“…Finally, the samples were analyzed with the above aptasensor. The recovery was calculated as follows: recovery rates = (concentration/spiked concentration) × 100% [28].…”

Section: Analysis Of Real Samplesmentioning

confidence: 99%

An AuNPs-Based Fluorescent Sensor with Truncated Aptamer for Detection of Sulfaquinoxaline in Water

et al. 2022

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Herein, we developed a novel truncation technique for aptamer sequences to fabricate highly sensitive aptasensors based on molecular docking and molecular dynamics simulations. The binding mechanism and energy composition of the aptamer/sulfaquinoxaline (SQX) complexes were investigated. We successfully obtained a new SQX-specific aptamer (SBA28-1: CCCTAGGGG) with high affinity (Kd = 27.36 nM) and high specificity determined using graphene oxide. This aptamer has a unique stem-loop structure that can bind to SQX. Then, we fabricated a fluorescence aptasensor based on SBA28-1, gold nanoparticles (AuNPs), and rhodamine B (RhoB) that presented a good linear range of 1.25–160 ng/mL and a limit of detection of 1.04 ng/mL. When used to analyze water samples, the aptasensor presented acceptable recovery rates of 93.1–100.1% and coefficients of variation (CVs) of 2.2–10.2%. In conclusion, the fluorescence aptasensor can accurately and sensitively detect SQX in water samples and has good application prospects.

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Selected ssDNA aptamers–graphene oxide‐based fluorescent biosensor to detect sulfameter in milk

Zhang

Tian

et al. 2023

Luminescence

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The abuse of sulfameter (SME) in animal husbandry can cause drug resistance and toxic or allergic reactions in humans. Therefore, it is very important to establish a simple, inexpensive, and efficient method for detecting SME in food. In this work, we propose a single fluorescent aptamer/graphene oxide (GO)‐based biosensor to detect SME residues in milk. Aptamers that specifically bind to SME were screened using capture‐SELEX and a ssDNA library immobilized on magnetic beads. The 68 active candidate aptamers were chemically synthesized for specificity and affinity characterization. Among the aptamers, the aptamer sulf‐1 revealed the highest affinity (Kd = 77 ± 15 nM) to SME and was selected to construct a GO‐based fluorescent biosensor for real milk sample detection. Under optimal conditions, the single fluorescent aptasensor had a wide linear range (R2 was 0.997) from 7 to 336 ng/ml and a low detection limit of 3.35 ng/ml that was calculated with a 3SD/slope. The single fluorescent method was also validated using SME‐fortified milk samples, showing average recoveries ranging from 99.01% to 104.60% with a relative standard deviation of less than 3.88%. These results demonstrate that this novel aptamer sensor provides an opportunity for sensitive, convenient, and accurate detection of SME residues in milk.

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A novel aptasensor based on Fe3O4/Au/g-C3N4 for sensitive detection of sulfameter in food matrices

Cited by 19 publications

References 21 publications

High-Sensitive FAM Labeled Aptasensor Based on Fe3O4/Au/g-C3N4 for the Detection of Sulfamethazine in Food Matrix

High-Sensitive FAM Labeled Aptasensor Based on Fe3O4/Au/g-C3N4 for the Detection of Sulfamethazine in Food Matrix

An AuNPs-Based Fluorescent Sensor with Truncated Aptamer for Detection of Sulfaquinoxaline in Water

Selected ssDNA aptamers–graphene oxide‐based fluorescent biosensor to detect sulfameter in milk

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