A novel assay for the determination of PreScission protease by capillary electrophoresis

Zhou, Shuwen; Cui, Pengfei; Sheng, Jie; Zhang, Xueli; Jiang, Pengju; Ni, Xiaomin; Cao, Kai; Qiu, Lin

doi:10.1016/j.bpc.2021.106696

Cited by 1 publication

(1 citation statement)

References 26 publications

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“…The same BFS capillary, bent to 1, 2, or 4 semicircularshaped turns with a 3-cm diameter and serving as a microreactor, was employed in a new assay for determination of PreScission protease activity in a microscale [260]. Due to different mobilities, the protease and the sub-strate (peptide labeled with ATTO 590 fluorescent dye and immobilized on GSH stabilized quantum dots) were mixed inside the bent capillary with sequential injections.…”

Section: Monitoring Of Chemical and Enzymatic Reactionsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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Section: Monitoring Of Chemical and Enzymatic Reactionsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

View full text Add to dashboard Cite

show abstract

A novel assay for the determination of PreScission protease by capillary electrophoresis

Cited by 1 publication

References 26 publications

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

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