A novel Bacillus cereus bacteriophage DLn1 and its endolysin as biocontrol agents against Bacillus cereus in milk

Li, Na; Yuan, Xiaoming; Li, Chun; Wang, Juan; Chen, Bo; Yu, Shuai; Yu, Pengfei; Zhang, Junhui; Zeng, Haiyang; Wu, Shi; Yang, Xiaojuan; Yang, Meiyan; Zhang, Jumei; Wu, Qingping; Ding, Yu

doi:10.1016/j.ijfoodmicro.2022.109615

Cited by 26 publications

(4 citation statements)

References 70 publications

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“…Phages generally present a specific host range, whereas endolysins possess a much broader lytic spectrum than their parental phages [ 49 , 50 ]. Our results support these existing conclusions by showing that LysSYL could reduce the viable bacterial numbers (+++) by over 4 log10 units across all 115 tested strains (100%), including S. epidermidis , S. haemolyticus , S. hominis , S. capitis , and most major clones of S. aureus .…”

Section: Discussionmentioning

confidence: 99%

LysSYL: a broad-spectrum phage endolysin targeting Staphylococcus species and eradicating S. aureus biofilms

Liu,

Wei,

Wang

et al. 2024

Microb Cell Fact

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Background Staphylococcus aureus and its single or mixed biofilm infections seriously threaten global public health. Phage therapy, which uses active phage particles or phage-derived endolysins, has emerged as a promising alternative strategy to antibiotic treatment. However, high-efficient phage therapeutic regimens have yet to be established. Results In this study, we used an enrichment procedure to isolate phages against methicillin-resistant S. aureus (MRSA) XN108. We characterized phage SYL, a new member of the Kayvirus genus, Herelleviridae family. The phage endolysin LysSYL was expressed. LysSYL demonstrated stability under various conditions and exhibited a broader range of efficacy against staphylococcal strains than its parent phage (100% vs. 41.7%). Moreover, dynamic live/dead bacterial observation demonstrated that LysSYL could completely lyse MRSA USA300 within 10 min. Scan and transmission electron microscopy revealed evident bacterial cell perforation and deformation. In addition, LysSYL displayed strong eradication activity against single- and mixed-species biofilms associated with S. aureus. It also had the ability to kill bacterial persisters, and proved highly effective in eliminating persistent S. aureus when combined with vancomycin. Furthermore, LysSYL protected BALB/c mice from lethal S. aureus infections. A single-dose treatment with 50 mg/kg of LysSYL resulted in a dramatic reduction in bacterial loads in the blood, liver, spleen, lungs, and kidneys of a peritonitis mouse model, which resulted in rescuing 100% of mice challenged with 108 colony forming units of S. aureus USA300. Conclusions Overall, the data provided in this study highlight the strong therapeutic potential of endolysin LysSYL in combating staphylococcal infections, including mono- and mixed-species biofilms related to S. aureus.

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Section: Discussionmentioning

confidence: 99%

LysSYL: a broad-spectrum phage endolysin targeting Staphylococcus species and eradicating S. aureus biofilms

Liu,

Wei,

Wang

et al. 2024

Microb Cell Fact

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“…DLn1 belongs to the podovirus morphotype, while Kirov belongs to the siphovirus morphotype. DLn1 demonstrated high thermostability and pH stability and was able to reduce the CFU count of B. cereus in contaminated milk by up to 10 4 -fold [ 40 ]. The bacteriophage Kirov effectively prevents the contamination of milk with ATCC 4342 at a concentration of 10 4 PFU/mL ( Figure 9 ).…”

Section: Discussionmentioning

confidence: 99%

A Genomic Analysis of the Bacillus Bacteriophage Kirovirus kirovense Kirov and Its Ability to Preserve Milk

Kazantseva

Skorynina

Piligrimova

et al. 2023

IJMS

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Bacteriophages are widely recognized as alternatives to traditional antibiotics commonly used in the treatment of bacterial infection diseases and in the food industry, as phages offer a potential solution in combating multidrug-resistant bacterial pathogens. In this study, we describe a novel bacteriophage, Kirovirus kirovense Kirov, which infects members of the Bacillus cereus group. Kirovirus kirovense Kirov is a broad-host-range phage belonging to the Caudoviricetes class. Its chromosome is a linear 165,667 bp double-stranded DNA molecule that contains two short, direct terminal repeats, each 284 bp long. According to bioinformatics predictions, the genomic DNA contains 275 protein-coding genes and 5 tRNA genes. A comparative genomic analysis suggests that Kirovirus kirovense Kirov is a novel species within the Kirovirus genus, belonging to the Andregratiavirinae subfamily. Kirovirus kirovense Kirov demonstrates the ability to preserve and decontaminate B. cereus from cow milk when present in milk at a concentration of 104 PFU/mL. After 4 h of incubation with the phage, the bacterial titer drops from 105 to less than 102 CFU/mL.

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“…Phages generally present a speci c host range, whereas endolysins possess a much broader lytic spectrum than their parental phages 36,37 . Our results support these existing conclusions by showing that LysSYL could reduce the viable bacterial numbers (+++) by over 4 log10 units across all 115 tested strains, including S. epidermidis, S. haemolyticus, S. hominis, S. capitis, and most major clones of S. aureus.…”

Section: Discussionmentioning

confidence: 99%

A novel phage endolysin inactivates a wide range of Staphylococcus aureus and eliminates mono- and mixed-species biofilms associated with S. aureus

Rao,

Liu,

Wei

et al. 2023

Preprint

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Staphylococcus aureus and its single or mixed biofilm infections seriously threaten global public health. Phage therapy, which uses active particles or phage-derived endolysins, has emerged as a promising alternative strategy to antibiotic treatment. However, high-efficient phage therapeutic regimens have yet to be established. In this study, we used a mixed-sample procedure to isolate phages against methicillin-resistant S. aureus (MRSA) XN108. We characterized a new phage, SYL, sequenced its genomes, and engineered its novel endolysin, LysSYL. LysSYL demonstrated stability under various conditions and exhibited a broader range of efficacy against staphylococcal strains than its parent phage (100% vs. 41.7%). Moreover, dynamic live/dead bacterial observation demonstrated that LysSYL could completely inactivate MRSA USA300 within 10 min. Scan and transmission electron microscopy revealed evident bacterial cell perforation and deformation. In addition, LysSYL displayed strong eradication activity against single- and mixed-species biofilms associated with S. aureus. It also had the ability to disrupt bacterial persisters, and it proved highly effective in eliminating persistent S. aureus when combined with vancomycin. Furthermore, LysSYL protected BALB/c mice from lethal S. aureus infections. A single-dose treatment with 50 mg/kg of LysSYL resulted in a dramatic reduction in bacterial loads in the blood, liver, spleen, lungs, and kidneys of a peritonitis mouse model, which resulted in rescuing 100% of mice challenged with 108 colony forming units of S. aureus USA300. Overall, the data provided in this study highlight the strong therapeutic potential of endolysin LysSYL in combating staphylococcal infections, including mono- and mixed-species biofilms related to S. aureus.

show abstract

A novel Bacillus cereus bacteriophage DLn1 and its endolysin as biocontrol agents against Bacillus cereus in milk

Cited by 26 publications

References 70 publications

LysSYL: a broad-spectrum phage endolysin targeting Staphylococcus species and eradicating S. aureus biofilms

LysSYL: a broad-spectrum phage endolysin targeting Staphylococcus species and eradicating S. aureus biofilms

A Genomic Analysis of the Bacillus Bacteriophage Kirovirus kirovense Kirov and Its Ability to Preserve Milk

A novel phage endolysin inactivates a wide range of Staphylococcus aureus and eliminates mono- and mixed-species biofilms associated with S. aureus

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