A novel biamperometric biosensor for urinary oxalate determination using flow-injection analysis

Milardović, Stjepan; Kereković, Irena; Nodilo, Marijana

doi:10.1016/j.talanta.2008.06.020

Cited by 24 publications

(15 citation statements)

References 29 publications

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“…Many solid materials have been employed for this purpose, and the best enzyme loading so far discovered is 182 mg g −1 on alkylamine glass beads; however, the activity of the immobilized OxOx was only 5.17% that of the free OxOx 11c. The high activity of the immobilized OxOx on CRGO can dramatically decrease the cost of using free OxOx in clinical oxalate detection and determination 21. The higher enzyme loading, better stability, and high activity of the immobilized OxOx suggested that CRGO is a promising immobilization substrate for proteins and that structural alteration caused by dehydration is less prominent 14b, 18…”

Section: Resultsmentioning

confidence: 99%

Assembly of Graphene Oxide–Enzyme Conjugates through Hydrophobic Interaction

Zhang

Huang

et al. 2011

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Biochemical and biomedical applications of graphene oxide (GO) critically rely on the interaction of biomolecules with it. It has been previously reported that the biological activity of the GO-enzyme conjugate decreases due to electrostatic interaction between the enzymes and GO. Herein, the immobilization of horseradish peroxidase (HRP) and oxalate oxidase (OxOx) on chemically reduced graphene oxide (CRGO) are reported. The enzymes can be adsorbed onto CRGO directly with a tenfold higher enzyme loading than that on GO, and maximum enzyme loadings reach 1.3 and 12 mg mg(-1) for HRP and OxOx, respectively. Significantly, the more CRGO is reduced, the higher the enzyme loading. The CRGO-HRP conjugates also exhibit higher enzyme activity and stability than GO-HRP. Excellent properties of the CRGO-enzyme conjugates are attributed to hydrophobic interaction between the enzymes and the CRGO. The hydrophobic interaction mode of the CRGO-enzyme conjugates can be applied to other hydrophobic proteins, and thus could dramatically improve the performance of immobilized proteins. The results indicate that CRGO is a potential substrate for efficient enzyme immobilization, and is an ideal candidate as a macromolecule carrier and biosensor.

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Section: Resultsmentioning

confidence: 99%

Assembly of Graphene Oxide–Enzyme Conjugates through Hydrophobic Interaction

Zhang

Huang

et al. 2011

Small

252

216

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“…Separation methods [ 2 , 4 , 5 , 6 , 7 , 8 ] and methods employing oxalate oxidase enzyme [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ] are the most widely used for oxalate determination. Classical methods for oxalate determination include capillary electrophoresis [ 2 ] and chromatographic methods [ 4 , 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Determination of Food Oxalates Using Silica–Titania Xerogel Modified with Eriochrome Cyanine R

Morosanova

Samodelov

Моросанова

2018

Sensors

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The interaction of silica–titania xerogel with triphenylmethane dyes (pyrocatechol violet, chrome azurol S, eriochrome cyanine R) has been investigated to create a new sensor material for solid phase spectrophotometric determination of food oxalates. The complex forming reaction between xerogel incorporated titanium(IV) and triphenylmethane dyes has been studied; half-reaction periods, complex composition, equilibrium constants, and xerogel sorption capacity have been calculated for each dye. Eriochrome cyanine R (ECR) is characterized by the shortest half-reaction period, the smallest equilibrium constant, and the greatest capacity; it has been chosen for the sensor material construction because titanium(IV)-ECR complex is formed faster and can be destroyed easier than other studied complexes. The interaction of this sensor material with oxalates has been described: the presence of oxalates causes sensor material discoloration and the absorbance is used as analytical signal. The analytical range is 35–900 mg/L (LOD 10.5 mg/L, n = 7). High concentrations of interfering inorganic anions, organic acids, and sucrose did not affect oxalate determination. Proposed solid phase spectrophotometric procedure has been successfully applied for the determination of oxalates in food samples (sorrel, spinach, parsley, ginger, and black pepper) and the results are in good agreement with HPLC oxalate determination.

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“…The ingestion of a large quantity of food rich in oxalic acid can cause loss of calcium in the blood as well as injury to the kidneys [43,46]. Many methods have been recommended for oxalate determination in clinical laboratory analyses but some of them are time-consuming (as chromatographic and spectrophotometric) while some others need a chemically pre-treated sample [42].…”

Section: Biosensor Applications In the Food Industrymentioning

confidence: 99%