A Novel Biological Agent Cytosinpeptidemycin Inhibited the Pathogenesis of Tobacco Mosaic Virus by Inducing Host Resistance and Stress Response

An, Mengnan; Zhao, Xing‐Ming; Zhou, Tao; Wang, Guanzhong; Xia, Zihao; Wu, Yuanhua

doi:10.1021/acs.jafc.9b02662

Cited by 16 publications

(25 citation statements)

References 51 publications

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“…Induction of ZAT12 is indicated to be closely related with the oxidative stress response, and serves as an abiotic stress marker [43]. Our previous work has shown that the biological agent of cytosinpeptidemycin significantly induced up-regulation of ZAT12 [20]. In this work, gene expression of ZAT12 was significantly up-regulated under NNM treatment.…”

Section: Discussionmentioning

confidence: 66%

“…The extracted RNA of each treatment group was adjusted to 10 μg and subjected to northern blot analysis as described [19] using a DIG High Prime DNA Labeling and Detection Starter Kit II (Roche, Mannheim, Germany). The digoxigenin-labelled RNA probes for TMV positive- and negative-strand RNA have been previously described [20]. The RNA signals were detected with a chemical luminous imaging system, Tanon 5200 (Tanon, Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

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Molecular Regulation of Host Defense Responses Mediated by Biological Anti-TMV Agent Ningnanmycin

Zhou

Guo

et al. 2019

Viruses

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Ningnanmycin (NNM) belongs to microbial pesticides that display comprehensive antiviral activity against plant viruses. NNM treatment has been shown to efficiently delay or suppress the disease symptoms caused by tobacco mosaic virus (TMV) infection in local-inoculated or systemic-uninoculated tobacco leaves, respectively. However, the underlying molecular mechanism of NNM-mediated antiviral activity remains to be further elucidated. In this study, 414 differentially expressed genes (DEGs), including 383 which were up-regulated and 31 down-regulated, caused by NNM treatment in TMV-infected BY-2 protoplasts, were discovered by RNA-seq. In addition, KEGG analysis indicated significant enrichment of DEGs in the plant–pathogen interaction and MAPK signaling pathway. The up-regulated expression of crucial DEGs, including defense-responsive genes, such as the receptor-like kinase FLS2, RLK1, and the mitogen-activated protein kinase kinase kinase MAPKKK, calcium signaling genes, such as the calcium-binding protein CML19, as well as phytohormone responsive genes, such as the WRKY transcription factors WRKY40 and WRKY70, were confirmed by RT-qPCR. These findings provided valuable insights into the antiviral mechanisms of NNM, which indicated that the agent induces tobacco systemic resistance against TMV via activating multiple plant defense signaling pathways.

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Section: Discussionmentioning

confidence: 66%

Section: Methodsmentioning

confidence: 99%

Molecular Regulation of Host Defense Responses Mediated by Biological Anti-TMV Agent Ningnanmycin

Zhou

Guo

et al. 2019

Viruses

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“…8 Some natural antivirals are also being commercialized, such as ningnanmycin 9 and cytosinpeptidemycin. 10 However, the creation and use of antivirals are still extremely limited as a result of complex syntheses, the ready decomposition of active components, their relatively low efficiency, high toxicity in non-target organisms, considerable pesticide resistance, and pesticide interactions. 11 Consequently, the development of novel, efficient, and environmentally friendly new antivirals is a top priority.…”

Section: ■ Introductionmentioning

confidence: 99%

Design, Synthesis, and Mechanism of Antiviral Acylurea Derivatives Containing a Trifluoromethylpyridine Moiety

Guo

Zhao

Wang

et al. 2021

J. Agric. Food Chem.

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Novel acylurea derivatives 7a–7ab were designed and synthesized by linking the active substructures trifluoromethylpyridine and anthranilic diamide via an acylurea bridge. Most of the title compounds exhibited good activity against tobacco mosaic virus (TMV), particularly compound 7x (EC50 of 211.8 μg/mL), which showed much higher curative activity than ningnanmycin (EC50 of 389.8 μg/mL), and compound 7ab, which showed excellent inactivation activity (EC50 of 36.1 μg/mL), similar to ningnanmycin (EC50 of 23.2 μg/mL). The preliminary mechanism of these derivatives was investigated. Autodocking analysis revealed that compounds 7x and 7ab had good affinity for TMV coat protein (TMV CP), with low binding energies (−7.86 and −8.59 kcal/mol) comparable to ningnanmycin (−8.75 kcal/mol). Molecular dynamics simulation showed that compound 7x had a stable system structure with a better binding free energy (−32.94 kcal/mol) than ningnanmycin (−25.62 kcal/mol). Microscale thermophoresis showed that compound 7x bound more strongly to TMV CP (K d of 19.8 ± 7.3 μM) than ningnanmycin (K d of 21.2 ± 7.3 μM). Transmission electron microscopy and self-assembly experiments demonstrated that compounds 7x and 7ab significantly obstructed the self-assembly of TMV RNA and TMV CP. This new acylurea derivative has excellent antiviral activity by targeting TMV CP and inhibiting TMV self-assembly and can be considered a candidate for antiviral applications.

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“…Plant viruses rely on interaction with multiple viral hosts for self‐multiplication and systemic infection 37 . Plant antiviral medicines can directly act on nucleic acid or viral coat protein to inhibit viruses, or indirectly change the balance of virus‐host interaction by regulating host response 38 . Previous studies have shown that extracellular proteins produced by beneficial microorganisms have antiviral activity.…”

Section: Discussionmentioning

confidence: 99%

BLB8, an antiviral protein from Brevibacillus laterosporus strain B8, inhibits Tobacco mosaic virus infection by triggering immune response in tobacco

Jiao

Shi

et al. 2021

Pest Management Science

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BACKGROUND Tobacco mosaic virus (TMV) is one of destructive plant viruses, causing serious economic losses in the world. Using antiviral proteins or elicitors to inhibit viral infection or promote plant immunity is one of the efficient strategies against TMV. Our previous study identified that the fermentation broth of Brevibacillus laterosporus strain B8 showed strong antiviral activity against TMV. However, the active antiviral ingredient is still unclear. RESULTS Here, BLB8 (B. laterosporus strain B8 protein, BLB8), an antiviral protein from B. laterosporus strain B8 was isolated and characterized. BLB8 showed protective, inactive and curative effects against TMV, and the inhibition rate reached up to 63%, 83% and 55%, respectively. BLB8 infiltrated around the infection site of the recombinant virus TMV‐GFP inhibited the systemic extend and movement of TMV. Pretreatment of the bottom leaves with BLB8 inhibited the spread and accumulation of TMV in upper systemic leaves. Furthermore, BLB8 caused hypersensitive response (HR) in a dose‐dependent way, promoted H2O2 accumulation, and induced the expression of defense‐relative genes in Nicotiana benthamiana. CONCLUSION The antiviral protein BLB8 from B. laterosporus strain B8 effectively inhibits TMV infection in inactivation, protective and curative effects through triggering plant immunity in tobacco. Therefore, the present study provides a new antiviral agent for prevention and control of viral disease. © 2021 Society of Chemical Industry.

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A Novel Biological Agent Cytosinpeptidemycin Inhibited the Pathogenesis of Tobacco Mosaic Virus by Inducing Host Resistance and Stress Response

Cited by 16 publications

References 51 publications

Molecular Regulation of Host Defense Responses Mediated by Biological Anti-TMV Agent Ningnanmycin

Molecular Regulation of Host Defense Responses Mediated by Biological Anti-TMV Agent Ningnanmycin

Design, Synthesis, and Mechanism of Antiviral Acylurea Derivatives Containing a Trifluoromethylpyridine Moiety

BLB8, an antiviral protein from Brevibacillus laterosporus strain B8, inhibits Tobacco mosaic virus infection by triggering immune response in tobacco

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