A Novel Biomimetic Approach to Repair Enamel Cracks/Carious Damages and to Reseal Dentinal Tubules by Amorphous Polyphosphate

Müller, Wernér E.G.; Ackermann, Maximilian; Neufurth, Meik; Tolba, Emad; Wang, Shunfeng; Feng, Qingling; Schröder, Heinz C.; Wang, Xiaohong

doi:10.20944/preprints201702.0010.v1

Cited by 4 publications

(2 citation statements)

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“…Mg 2+ ions). Similar types of particulates containing PolyPs and inorganic divalent cations have also been observed in nature, such as granules in mitochondria, while synthetic analogues can be prepared in alkaline solutions with excess cations [61][62][63][64] for use in applications of bone regeneration 65 and cartilage repair 64 , among others.…”

Section: Microfluidics Analysis Of Crystal Growth Inhibitionmentioning

confidence: 91%

Engaging a Battle on Two Fronts: Dual Role of Polyphosphates as Potent Inhibitors of Struvite Nucleation and Crystal Growth

et al. 2020

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Polyprotic acids often serve as effective regulators of inorganic crystallization in natural, biological, and synthetic systems.These molecules or macromolecules can function as efficient modifiers that either inhibit or promote crystal growth. Few studies report potent inhibitors of crystal nucleation owing to the presumed challenges of hindering these stochastic events; however, in this study we have identified polyphosphates that operate as potent inhibitors of both nucleation and crystal growth of the natural mineral struvite (MgNH4PO4ˑ6H2O), which is a common component of scale in commercial and physiological processes (e.g. water purification and kidney disease). Systematic investigation of linear and cyclic polyphosphates of varying molecular weight reveal that micromolar concentrations of three modifiers, hexametaphosphate, polyphosphate type 45, and phytic acid, lead to suppressed struvite nucleation. Combined studies of struvite growth at the macroscopic level using microfluidics to track anisotropic rates of crystallization and at the molecular level using in situ atomic force microscopy (AFM) to monitor layered growth of crystal surfaces collectively reveal that these modifiers are also capable of fully suppressing crystal growth via their affinity to interact with all principal crystal facets. Time-resolved AFM images of the (101) surface at varying polyphosphate concentrations reveal a unique mode of crystal growth inhibition wherein surfaces become laden with an amorphous layer that leads to roughened interfaces and growth succession through dynamic sequences that are not commonly witnessed for other minerals. It is also observed under certain conditions that inhibitors can induce significant changes in crystal morphology, leading to exquisite leaf-like hierarchical structures. The discovery of potent inhibitors of struvite formation that operate by unparalleled dual modes of action capable of suppressing both nucleation and crystal growth has potential implications for their use in the prevention of commercial scale in water purification as well as in the treatment of kidney stones. Moreover, the fundamental mechanisms of polyphosphates identified in this study may prove to be relevant for other inorganic crystals given the ubiquitous use of inhibitors in processes spanning from biomineralization to crystal engineering.

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Section: Microfluidics Analysis Of Crystal Growth Inhibitionmentioning

confidence: 91%

Engaging a Battle on Two Fronts: Dual Role of Polyphosphates as Potent Inhibitors of Struvite Nucleation and Crystal Growth

et al. 2020

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“…Collagen type I alpha 1 (COL1A1) is a type of collagen. Researchers have discovered that collagen is the important protein component in teeth, bones, the adhesion of tumor cell, and extracellular matrix (ECM) [ 15 , 16 , 17 ]. In previous studies, COL1A1 was upregulated in cervical cancer cells and restrained cell apoptosis [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

CircRNA circ_0004370 promotes cell proliferation, migration, and invasion and inhibits cell apoptosis of esophageal cancer via miR-1301-3p/COL1A1 axis

et al. 2021

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Background The aim of this study was to investigate the circ_0004370 expression in EC, its effects on cell proliferation, apoptosis, migration, invasion, and epithelial–mesenchymal transition (EMT) process, and the underlying regulatory mechanisms in EC. Methods The protein levels of COL1A1 and EMT-related proteins were detected by western blot. The role of circ_0004370 on cell viability, proliferation, and apoptosis was analyzed by Cell Counting Kit-8 (CCK-8) assay, colony formation assay, and flow cytometry, respectively. The transwell assay was used to examine cell migration and invasion. The binding sites between miR-1301-3p and circ_0004370 or COL1A1 were predicted by starbase software and confirmed by dual-luciferase reporter assay and RNA pull-down assay. Results We discovered that circ_0004370 was remarkably upregulated in EC tissues and cells. Knockdown of circ_0004370 inhibited cell proliferation, migration as well as invasion, and promoted apoptosis in vitro, while its effect was rescued by miR-1301-3p inhibition. And circ_0004370 mediated the EMT process in EC cells. Moreover, we explored its regulatory mechanism and found that circ_0004370 directly bound to miR-1301-3p and COL1A1 was verified as a target of miR-1301-3p. COL1A1 was highly expressed in EC cells and upregulation of COL1A1 reversed the effects of miR-1301-3p on cell proliferation, migration, invasion, and apoptosis. In addition, silencing of circ_0004370 reduced tumor volumes and weights in vivo. We showed that circ_0004370/miR-1301-3p/COL1A1 axis played the critical role in EC to regulate the cell activities. Conclusion Circ_0004370 promotes EC proliferation, migration and invasion, and EMT process and suppresses apoptosis by regulating the miR-1301-3p/COL1A1 axis, indicating that circ_0004370 may be used as a potential therapeutic target for EC.

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Advances in biomimetic mineralization of tooth enamel based on cell-free strategies

Shi

2022

MATEC Web Conf.

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Tooth enamel is a highly-mineralized hard tissue covering the outermost layer of the dental crown, and amelogenesis is inseparable from the participation of necessary components such as ameloblasts, organic matrix proteins, and mineral ions, such as Ca2+ and PO43-. However, mature enamel is an acellular tissue and it is difficult to self-repair once damaged. The current treatment methods for enamel damage are filling or repairing with alloys, ceramics, or composite resins. However, the mechanical properties of these materials are quite different from the natural enamel and they can’t ensure a completely closed interface with the remaining enamel surface, which usually causes a series of post-repair problems. At present, the biomimetic mineralization of tooth enamel is a research hotspot in the field of prosthodontics, and has great clinical application needs and prospects, especially the researches on cell-free strategies have made significant accomplishment. Here, based on the cell-free strategies, we review the recent knowledge from ex situ and in situ two dimensions in the remineralization of tooth ename.

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A Novel Biomimetic Approach to Repair Enamel Cracks/Carious Damages and to Reseal Dentinal Tubules by Amorphous Polyphosphate

Cited by 4 publications

References 54 publications

Engaging a Battle on Two Fronts: Dual Role of Polyphosphates as Potent Inhibitors of Struvite Nucleation and Crystal Growth

Engaging a Battle on Two Fronts: Dual Role of Polyphosphates as Potent Inhibitors of Struvite Nucleation and Crystal Growth

CircRNA circ_0004370 promotes cell proliferation, migration, and invasion and inhibits cell apoptosis of esophageal cancer via miR-1301-3p/COL1A1 axis

Advances in biomimetic mineralization of tooth enamel based on cell-free strategies

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