A Novel Biomolecule Sorter Using Thermosensitve Hydrogel in Micro Flow System

Shirasaki, Yoshitaka; Makazu, Hiroshi; Tashiro, Koichi; Ikeda, Shingo; Sekiguchi, Tetsushi; Shoji, Shuichi; Tsukita, Shöichiro; Funatsu, Takashi

doi:10.1007/978-94-010-0504-3_107

Cited by 15 publications

(3 citation statements)

References 1 publication

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“…Previous [9] thermosensitive hydrogel sheath flow system is 24 times faster than the previous sorter and the error is almost 0%. The switching time and error of the two-phase sheath flow sorter were less than 20 ms and 5-10%, respectively.…”

Section: Parametersmentioning

confidence: 87%

“…Previous studies have reported the fabrication of active hydrogel components 0957-0233/06/123141+06$30.00 © 2006 IOP Publishing Ltd Printed in the UK inside microchannels using direct photo patterning [5], yeast cells fixed by thermal gelation of a poly N-isopropylac-lamide (PNIPAAm) hydrogel and the microbes desired separated from the population [6], and a one-inlet and nine-outlet multi-sorting system using thermoreversible gelation of polymer (Mebiol Gel TM [7]) [8]. In our previous study of sorting, time was limited to about 120 ms [9]. This previous sorting system has been successfully operated to separate E. coli bacterial cells expressing GFP continuously for up to 6 h [10].…”

Section: Introductionmentioning

confidence: 99%

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High-speed particles and biomolecules sorting microsystem using thermosensitive hydrogel

Arakawa

Shirasaki

Izumi

et al. 2006

Meas. Sci. Technol.

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Two types of high-speed particle and biomolecule sheath flow sorters were realized using thermosensitive hydrogel. One is all hydrogel sheath flow and the other is two-phase sheath flow that consists of a water flow including samples and two hydrogel carrier flows; these have been utilized instead of the air and water droplet two-phase flow used in the conventional commercialized cell sorting system. Flow switching is performed by the sol–gel transfer of the thermosensitive hydrogel generated by focused infrared (IR) laser irradiation. High-speed sorting less than 5.0 ms and no error sorting (120 000 counts) in the all hydrogel sheath flow system while 20 ms in the two-phase sheath flow was realized. Since sorting was performed in simple PDMS-glass microchannels without any electric stimulation and mechanical valve structures, the proposed system is suitable for many biochemical applications.

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Section: Parametersmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

High-speed particles and biomolecules sorting microsystem using thermosensitive hydrogel

Arakawa

Shirasaki

Izumi

et al. 2006

Meas. Sci. Technol.

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“…However, because this system tends to be large and expensive, a low-cost cell sorter has been developed on a microchip. There are many methods of microscale cell sorting, such as dielectrophoretic sorting [8][9][10], laser trap [11], magnetic isolation, and switching in microfluidic channels using microvalves [12]. It is necessary to design the system to fit the characteristics of the sorting object (e.g., size), the condition of the carrier liquid, and the sorting speed.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible polymeric magnetically driven microtool for particle sorting

Yamanishi

Sakuma

Onda

et al. 2008

J. Micro-Nano Mech.

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The paper describes a novel non-contact sorting system using an on-chip, magnetically driven micro-tool (MMT) rather than conventional biomanipulation by hand, which has a higher risk of contamination and lower success rate and repeatability. MMTs can sort particles individually, and have the unique feature that they can be installed directly in a microchannel (width = 150 µm), unlike other conventional cell sorting systems based on field gradients or electrostatic force. The proposed method can be applied to other micro-objects independently of dielectric properties and without the complexity and power requirement associated with optical means. The drive unit was significantly downsized by amplifying the density of magnetic flux using a permanent magnet. Lower power consumption could also be realized, because no energy is required to keep MMTs stationary at a particular position. Sorting was performed using real-time sensing images of microbeads, where the system successfully sorted beads of different sizes at switching speeds up to 18 Hz. The MMT developed in this study is unique with respect to its flexibility and biocompatibility; in addition, since the PDMS (polydimethylsiloxane) microchip is disposable, it can be applied to cell sorting without any risk of contamination. Potential fields of application of this technology include cloning techniques, which require sorting of oocytes with and without nuclei.

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Non-Contact On-Chip Manipulation of 3-D Microtools and Its Applications

Arai

Yamanishi

2010

Next-Generation Actuators Leading Breakthroughs

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A Novel Biomolecule Sorter Using Thermosensitve Hydrogel in Micro Flow System

Cited by 15 publications

References 1 publication

High-speed particles and biomolecules sorting microsystem using thermosensitive hydrogel

High-speed particles and biomolecules sorting microsystem using thermosensitive hydrogel

Biocompatible polymeric magnetically driven microtool for particle sorting

Non-Contact On-Chip Manipulation of 3-D Microtools and Its Applications

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