A novel black poplar propolis extract with promising health-promoting properties: focus on its chemical composition, antioxidant, anti-inflammatory, and anti-genotoxic activities

Acito, Mattia; Varfaj, Ina; Brighenti, Virginia; Cengiz, Emine Ceren; Rondini, Tommaso; Fatigoni, Cristina; Russo, Carla; Pietrella, Donatella; Pellati, Federica; Bartolini, Desirée; Sardella, Roccaldo; Moretti, Massimo; Villarini, Milena

doi:10.1039/d3fo05059a

Food Funct.

2024

DOI: 10.1039/d3fo05059a

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A novel black poplar propolis extract with promising health-promoting properties: focus on its chemical composition, antioxidant, anti-inflammatory, and anti-genotoxic activities

Mattia Acito,

Ina Varfaj,

Virginia Brighenti

et al.

Abstract: Propolis is a resinous mixture produced by honeybees, which has been used since ancient times for its useful properties. However, its chemical composition and bioactive activity may vary, depending on...

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Cited by 3 publications

References 99 publications

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The effect of extraction conditions on the chemical profile of obtained raw poplar propolis extract

Miłek,

Bonikowski,

Dżugan

2024

Chem. Pap.

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Various conditions of extraction were applied to obtain active extracts of raw poplar-type propolis. The extraction efficiency of traditional maceration was evaluated in terms of used solvent: ethanol (70 and 96% v/v), acetone (pure and 70%), propylene glycol, glycerol (50%), water and water with extraction modifiers: PEG 400 and lecithin. For obtained extracts, the total amounts of extracted phenolics and flavonoids as well as antioxidant activity were compared. For the most active extracts, the profile of volatile organic compounds with the use of GC × GC–MS and selected polyphenols content by HPLC–DAD was compared. To increase the activity of water propolis, extract ultrasound and microwave-assisted extraction were applied and obtained water extracts were compared regarding the main polyphenolic compounds content quantified by HPLC method. The recovery of 70% ethanolic extraction and the effect of the extension of extraction time were also examined by the HPTLC method. Based on conducted analyses, 70% ethanolic extract was found as the most aromatic and bioactive, followed by pure acetone and polypropylene glycol extracts. Compared to the classic maceration, water extraction assisted by microwaves and ultrasounds did not provide a higher extraction yield. In the case of 70% ethanolic extraction, the 5-day duration is recommended which allows to recovery of close to 80% of bioactive components of raw propolis.

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The effect of extraction conditions on the chemical profile of obtained raw poplar propolis extract

Miłek,

Bonikowski,

Dżugan

2024

Chem. Pap.

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Olive Mill Wastewater Extract: In Vitro Genotoxicity/Antigenotoxicity Assessment on HepaRG Cells

Rondini,

Branciari,

Franceschini

et al. 2024

IJERPH

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Olive mill wastewater (OMWW), with its high level of phenolic compounds, simultaneously represents a serious environmental challenge and a great resource with potential nutraceutical activities. To increase the knowledge of OMWW’s biological effects, with an aim to developing a food supplement, we performed a chemical characterisation of the extract using the Liquid Chromatography–Quadrupole Time-of-flight spectrometry (LC–QTOF) and an in vitro genotoxicity/antigenotoxicity assessment on HepaRG ™ cells. Chemical analysis revealed that the most abundant phenolic compound was hydroxytyrosol. Biological tests showed that the extract was not cytotoxic at the lowest tested concentrations (from 0.25 to 2.5 mg/mL), unlike the highest concentrations (from 5 to 20 mg/mL). Regarding genotoxic activity, when tested at non-cytotoxic concentrations, the extract did not display any effect. Additionally, the lowest tested OMWW concentrations showed antigenotoxic activity (J-shaped dose–response effect) against a known mutagenic substance, reducing the extent of DNA damage in the co-exposure treatment. The antigenotoxic effect was also obtained in the post-exposure procedure, although only at the extract concentrations of 0.015625 and 0.03125 mg/mL. This behaviour was not confirmed in the pre-exposure protocol. In conclusion, the present study established a maximum non-toxic OMWW extract dose for the HepaRG cell model, smoothing the path for future research.

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An Advanced Combinatorial System from Vitis vinifera Leaves and Propolis Enhances Antioxidants’ Skin Delivery and Fibroblasts Functionality

Athanasopoulou,

Spanidi,

Panagiotidou

et al. 2024

Pharmaceuticals

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Background/Objectives: Vine leaves are a bulky by-product that are disposed of and treated as waste in the wine production process. In the present study polyphenols from vine leaves were extracted and simultaneously encapsulated in a new delivery system consisting of liposomes and cyclodextrins. This system was further combined with propolis polyphenols encapsulated in cyclodextrins, resulting in a colloidal suspension for the release of antioxidants in a time-controlled way, the rate of which depends on the ratio of the materials. The result is a raw material that exhibits antioxidant and ECM protective effects when administered in skin fibroblasts (NHDFs). Methods: The antioxidant and ECM promoting efficacy of the produced raw material was assessed by the Folin–Ciocalteu method, DPPH assay, and in cellulo assays in fibroblasts, such as the cell viability assay, scratch assay, cell migration assay, gene expression analysis, and immunofluorescence analysis, for the detection, visualization, and quantification of collagen-I, collagen-IIIa, and elastin signals and collagenase assay. Results: Treatment of NHDFs with the combinatorial delivery system promoted collagen and elastin synthesis and deposition in normal conditions and, upon induced external stress, as assessed by in vitro transcriptomic and proteomic analysis. A significant inhibition of collagenase was also observed, suggesting a multitargeted efficacy of the active ingredients also by preventing collagen degradation. Conclusions: Therefore, this liposome–cyclodextrin encapsulated polyphenol complex represents a novel bioactive ingredient with promising skin applications.

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A novel black poplar propolis extract with promising health-promoting properties: focus on its chemical composition, antioxidant, anti-inflammatory, and anti-genotoxic activities

Abstract: Propolis is a resinous mixture produced by honeybees, which has been used since ancient times for its useful properties. However, its chemical composition and bioactive activity may vary, depending on...

Cited by 3 publications

References 99 publications

The effect of extraction conditions on the chemical profile of obtained raw poplar propolis extract

The effect of extraction conditions on the chemical profile of obtained raw poplar propolis extract

Olive Mill Wastewater Extract: In Vitro Genotoxicity/Antigenotoxicity Assessment on HepaRG Cells

An Advanced Combinatorial System from Vitis vinifera Leaves and Propolis Enhances Antioxidants’ Skin Delivery and Fibroblasts Functionality

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