A novel brain receptor is expressed in a distinct population of olfactory sensory neurons

Abstract

Link to publication in University of Groningen/UMCG research database Citation for published version (APA): Conzelmann, S., Levai, O., Bode, B., Eisel, U., Raming, K., Breer, H., & Strotmann, J. (2000). A novel brain receptor is expressed in a distinct population of olfactory sensory neurons. European Journal of Neuroscience, 12(11), 3926-3934. https://doi.org/10.1046Neuroscience, 12(11), 3926-3934. https://doi.org/10. /j.1460Neuroscience, 12(11), 3926-3934. https://doi.org/10. -9568.2000 Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. AbstractThree novel G-protein-coupled receptor genes related to the previously described RA1c gene have been isolated from the mouse genome. Expression of these genes has been detected in distinct areas of the brain and also in the olfactory epithelium of the nose. Developmental studies revealed a differential onset of expression: in the brain at embryonic stage 17, in the olfactory system at stage E12. In order to determine which cell type in the olfactory epithelium expresses this unique receptor type, a transgenic approach was employed which allowed a coexpression of histological markers together with the receptor and thus visualization of the appropriate cell population. It was found that the receptor-expressing cells were located very close to the basal membrane of the epithelium; however, the cells extended a dendritic process to the epithelial surface and their axons projected into the main olfactory bulb where they converged onto two or three glomeruli in the dorsal and posterior region of the bulb. Thus, these data provide evidence that this unique type of receptor is expressed in mature olfactory neurons and suggests that it may be involved in the detection of special odour molecules. IntroductionG-protein-coupled receptors (GPCRs) are integral membrane proteins which mediate signals to the interior of cells via activation of heterotrimeric G-proteins, which subsequently interact with and activate various effector proteins, ultimately resulting in the physiological response. GPCRs are involved in the transduction of a large variety of extracellular signals as diverse as inorganic ions, peptides and lipids or sensory stimuli like photons or odourants. More and more orphan G-protein-coupled receptors have been made available by various cloning procedures such as PCR ampli®cation or systematic sequencing of cDNA libraries (Marchese et al., 1999).We have recently isolated a cDNA...

Retinoic Acid Receptor-Dependent Survival of Olfactory Sensory Neurons in Postnatal and Adult Mice

“…Transgenic mice were crossed to P2-IRES-tauLacZ mice (Mombaerts et al, 1996), MOL2.3-IRES-tauLacZ-IRES-GFP (Conzelmann et al, 2000), or RARE-Hsp-LacZ mice. Tissues from the F1 generation of these crosses were processed and sectioned at 20 m as described above.…”

“…The P2 odorant receptor belongs to class II odorant receptors and is expressed in a medial zone of the epithelium, whereas MOL2.3 is a class I odorant receptor that is expressed in a dorsal zone of the epithelium (Mombaerts et al, 1996;Conzelmann et al, 2000). To verify that also class I receptor-expressing OSNs were reduced in number, we analyzed newborn and 1-month-old (PD28) progeny from OMP-dnRAR ϫ MOL2.3-IRES-tauLacZ crosses.…”

Maps of Odorant Molecular Features in the Mammalian Olfactory Bulb

“…Class I ORs (fish type, present in both fish and tetrapods) tend to recognize relatively hydrophilic compounds with one or more polar functional group(s), whereas tetrapod-specific class II ORs tend to recognize more hydrophobic compounds (48,54,60). Class I ORs are expressed in zone 1 of the olfactory epithelium, while class II ORs are expressed in all the four zones (14,17,54). Thus glomeruli in zone 1 represent both class I ORs and class II ORs, while glomeruli in zones 2-4 are most likely to represent class II ORs.…”

“…This raises the possibility that the alkylamine-responsive glomeruli in cluster A represent fishlike class I ORs (see sect. VI) (17,132).…”

Rodents and humans are able to detect the odour of L-Lactate

“…For example, in testis, ORs are thought to play a role in sperm chemotaxis [12, 13] but their roles in other tissues, including the brain [14, 15], are completely unknown. One of these ectopically expressed ORs is the mouse olfactory receptor 78 (Olfr78) which has rat and human orthologues (Olr59 and OR51E2, respectively [16, 17]). In the mouse, Olfr78 was detected in the brainstem area postrema and nucleus tractus solitarius [16] and later found in smooth muscle cells in small blood vessels in a variety of tissues [18, 19].…”