A novel C-type lectin in the black tiger shrimp Penaeus monodon functions as a pattern recognition receptor by binding and causing bacterial agglutination

Wongpanya, Ratree; Sengprasert, Panjana; Amparyup, Piti; Tassanakajon, Anchalee

doi:10.1016/j.fsi.2016.11.042

Cited by 47 publications

(19 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lectins have been shown to be directly relevant to the immune system of crustaceans [91, 92]. A C-type lectin (CTL) in M. japonicus , expressed primarily in intestinal tissues, is upregulated upon bacteria and WSSV infection and can bind LPS and PGN in a dose-dependent manner [93].…”

Section: Resultsmentioning

confidence: 99%

Comparative genomic analysis of innate immunity reveals novel and conserved components in crustacean food crop species

Lai

Aboobaker

2017

BMC Genomics

View full text Add to dashboard Cite

BackgroundGrowing global demands for crustacean food crop species have driven large investments in aquaculture research worldwide. However, large-scale production is susceptible to pathogen-mediated destruction particularly in developing economies. Thus, a thorough understanding of the immune system components of food crop species is imperative for research to combat pathogens.ResultsThrough a comparative genomics approach utilising extant data from 55 species, we describe the innate immune system of the class Malacostraca, which includes all food crop species. We identify 7407 malacostracan genes from 39 gene families implicated in different aspects of host defence and demonstrate dynamic evolution of innate immunity components within this group. Malacostracans have achieved flexibility in recognising infectious agents through divergent evolution and expansion of pathogen recognition receptors genes. Antiviral RNAi, Toll and JAK-STAT signal transduction pathways have remained conserved within Malacostraca, although the Imd pathway appears to lack several key components. Immune effectors such as the antimicrobial peptides (AMPs) have unique evolutionary profiles, with many malacostracan AMPs not found in other arthropods. Lastly, we describe four putative novel immune gene families, potentially representing important evolutionary novelties of the malacostracan immune system.ConclusionOur analyses across the broader Malacostraca have allowed us to not only draw analogies with other arthropods but also to identify evolutionary novelties in immune modulation components and form strong hypotheses as to when key pathways have evolved or diverged. This will serve as a key resource for future immunology research in crustacean food crops.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3769-4) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative genomic analysis of innate immunity reveals novel and conserved components in crustacean food crop species

Lai

Aboobaker

2017

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…This specificity is due to their high level of polymorphism and/or diversification to face up pathogens’ antigenic diversity (31). In addition to their role as soluble receptors, they can also limit the spreading of the pathogen in the host’s tissues and participate to its elimination (32, 33). Two different C-type lectins were interacting with the bacterium M. luteus and the yeast S. cerevisiae but not with the three other pathogens (Figure 2; Table S1 in Supplementary Material).…”

Section: Resultsmentioning

confidence: 99%

“…Indeed, most of the studies are based on the differential analysis (i.e., uninfected vs infected, or infected by different pathogens) of the host immune response (efficient or not) leading to a list of genes whose immunological function is rarely demonstrated. Moreover, if functional invalidation (gene knock-out, siRNA-mediated gene silencing, and mutants) already demonstrated the requirement of such molecules during the immune response, the first step of pathogens binding is still rarely studied (11, 12, 33). …”

Section: Resultsmentioning

confidence: 99%

Specific Pathogen Recognition by Multiple Innate Immune Sensors in an Invertebrate

et al. 2017

View full text Add to dashboard Cite

Detection of pathogens by all living organisms is the primary step needed to implement a coherent and efficient immune response. This implies a mediation by different soluble and/or membrane-anchored proteins related to innate immune receptors called PRRs (pattern-recognition receptors) to trigger immune signaling pathways. In most invertebrates, their roles have been inferred by analogy to those already characterized in vertebrate homologs. Despite the induction of their gene expression upon challenge and the presence of structural domains associated with the detection of pathogen-associated molecular patterns in their sequence, their exact role in the induction of immune response and their binding capacity still remain to be demonstrated. To this purpose, we developed a fast interactome approach, usable on any host–pathogen couple, to identify soluble proteins capable of directly or indirectly detecting the presence of pathogens. To investigate the molecular basis of immune recognition specificity, different pathogens (Gram-positive bacterium, Micrococcus luteus; Gram-negative, Escherichia coli; yeast, Saccharomyces cerevisiae; and metazoan parasites, Echinostoma caproni or Schistosoma mansoni) were exposed to hemocyte-free hemolymph from the gastropod Biomphalaria glabrata. Twenty-three different proteins bound to pathogens were identified and grouped into three different categories based on their primary function. Each pathogen was recognized by a specific but overlapping set of circulating proteins in mollusk’s hemolymph. While known PRRs such as C-type lectins were identified, other proteins not known to be primarily involved in pathogen recognition were found, including actin, tubulin, collagen, and hemoglobin. Confocal microscopy and specific fluorescent labeling revealed that extracellular actin present in snail hemolymph was able to bind to yeasts and induce their clotting, a preliminary step for their elimination by the snail immune system. Aerolysin-like proteins (named biomphalysins) were the only ones involved in the recognition of all the five pathogens tested, suggesting a sentinel role of these horizontally acquired toxins. These findings highlight the diversity and complexity of a highly specific innate immune sensing system. It paves the way for the use of such approach on a wide range of host–pathogen systems to provide new insights into the specificity and diversity of immune recognition by innate immune systems.

show abstract

“…Lectins have been shown to be directly relevant to the immune system of crustaceans (Wang et al, 2013; Wongpanya et al 2016). A C-type lectin in M. japonicus , expressed primarily in intestinal tissues, is upregulated upon bacteria and WSSV infection and can bind LPS and PGN in a dose-dependent manner (Feng et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

The innate immune systems of malacostracan crustaceans exhibit both conserved and evolutionarily distinct components

Lai

Aboobaker

2016

Preprint

View full text Add to dashboard Cite

A novel C-type lectin in the black tiger shrimp Penaeus monodon functions as a pattern recognition receptor by binding and causing bacterial agglutination

Cited by 47 publications

References 31 publications

Comparative genomic analysis of innate immunity reveals novel and conserved components in crustacean food crop species

Comparative genomic analysis of innate immunity reveals novel and conserved components in crustacean food crop species

Specific Pathogen Recognition by Multiple Innate Immune Sensors in an Invertebrate

The innate immune systems of malacostracan crustaceans exhibit both conserved and evolutionarily distinct components

Contact Info

Product

Resources

About