A novel cationic niosome formulation for gene delivery to the retina

Puras, Gustavo; Mashal, Mohamed; Zárate, Jon; Agirre, Mireia; Ojeda, E; Grijalvo, Santiago; Eritja, Ramón; Díaz-Tahoces, Ariadna; Navarrete, Gema Martínez; Avilés‐Trigueros, Marcelino; Fernández, Eduardo; Pedraz, José Luís

doi:10.1016/j.jconrel.2013.11.004

Cited by 139 publications

(132 citation statements)

References 45 publications

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“…Research has been focused on providing controlled release of antimicrobial agents, anti-inflammatory drugs, peptides and various macromolecules (156)(157)(158)(159)(160). Cationic niosomes have been prepared by the combination of cationic lipids and non-ionic surfactants in niosomes (161). Recently, novel multicomponent drug delivery systems have been prepared in which preformed niosomes were loaded into liquid crystal gel.…”

Section: Niosomesmentioning

confidence: 99%

Liposomal Drug Delivery: A Versatile Platform for Challenging Clinical Applications

et al. 2014

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-Liposomes are lipid based vesicular systems that offer novel platform for versatile drug delivery to target cell. Liposomes were first reported by Bangham and his co-workers in 1964 (1). Since then, liposomes have undergone extensive research with the prime aim to optimize encapsulation, stability, circulation time and target specific drug delivery. Manipulation of a liposome's lipid bilayer and surface decoration with selective ligands has transformed conventional liposomes into adaptable and multifunctional liposomes. Development of liposomes with target specificity provide the prospect of safe and effective therapy for challenging clinical applications. Bioresponsive liposomes offer the opportunity to release payload in response to tissue specific microenvironment. Incorporation of novel natural and synthetic materials has extended their application from stable formulations to controlled release targeted drug delivery systems. Integration and optimization of multiple features into one system revolutionized research in the field of cancer, gene therapy, immunotherapy and infectious diseases. After 50 years since the first publication, this review is aimed to highlight next generation of liposomes, their preparation methods and progress in clinical applications.

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Section: Niosomesmentioning

confidence: 99%

Liposomal Drug Delivery: A Versatile Platform for Challenging Clinical Applications

et al. 2014

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“…They have the potential to offer advantages over traditional liposomes, owing to the high purity of the surfactants, lower cost, greater chemical and physical stability, extended shelf-life, wider formulation versatility and greater possibilities for sterilization (Hasan et al, 2013). However, the greatest advantage of these innovative carriers is the possibility of inserting in their structure suitable agents appropriately functionalized in order to interact with specific biological targets (Sankar et al, 2009;Bragagni et al, 2014;Puras et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Sugar-based novel niosomal nanocarrier system for enhanced oral bioavailability of levofloxacin

et al. 2016

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Context: Vesicular systems have attracted great attention in drug delivery because of their amphiphilicity, biodegradability, non-toxicity and potential for increasing drug bioavailability. Objective: A novel sugar-based double-tailed surfactant containing renewable block was synthesized for preparing niosomal vesicles that could be exploited for Levofloxacin encapsulation, aiming to increase its oral bioavailability. Materials and methods: The surfactant was characterized by 1 H NMR, mass spectroscopy and Fourier transform infrared spectroscopy (FT-IR). Its biocompatibility was studied against cell cultures and human blood hemolysis. In vivo acute toxicity was evaluated in mice. The vesicle morphology, size, drug-excipients interaction and entrapment efficiency (EE) were examined using atomic force microscope (AFM), dynamic light scattering (DLS), FT-IR and HPLC. Oral bioavailability studies of Levofloxacin in surfactant-based niosomal formulation were carried out using rabbits and plasma samples were analyzed using HPLC. Results and discussion: Vesicles were spherical in shape and the size was 190.31 ± 4.51 nm with a polydispersity index (PDI) of 0.29 ± 0.03. The drug EE in niosomes was 68.28 ± 3.45%. When applied on cell lines, high cell viability was observed even after prolonged exposure at high concentrations. It caused 5.77 ± 1.34% hemolysis at 1000 mg/mL and was found to be safe up to 2000 mg/kg. Elevated Levofloxacin plasma concentration was achieved when delivered with novel vesicles. Conclusion:The surfactant was demonstrated to be safe and effective as carrier of Levofloxacin. The study suggests that this sugar-based double-tailed nonionic surfactant could be promising nano-vesicular system for delivery and enhancing oral bioavailability of the hydrophobic Levofloxacin.

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“…Despite recent improvements in plasmid DNA delivery, regarding efficiency and viability, the efficiency of transfection is still considered to be low or moderate (seldom over 50%) (31,32). Moreover, the most successful in vitro plasmid DNA gene delivery studies were on RPE-derived cell lines, which are easier to transfect than primary RPE cells (33).…”

Section: Discussionmentioning

confidence: 99%

Efficient Delivery and Functional Expression of Transfected Modified mRNA in Human Embryonic Stem Cell-derived Retinal Pigmented Epithelial Cells

Hansson

Albert

Somermeyer

et al. 2015

Journal of Biological Chemistry

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Background:In vitro-produced retinal pigmented epithelial (RPE) cells represent a novel source for retinal degenerative disease healing. Results: mRNA transfection outperformed plasmid transfection in cellular uptake. Modified-mRNA displayed negligible immune activation and functional protein expression. Conclusion: Modified mRNA transfection can be used efficiently for the engineering of RPE cells. Significance: The modified mRNA transfection technique offers new venues for the treatment of RPE-related diseases.

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A novel cationic niosome formulation for gene delivery to the retina

Cited by 139 publications

References 45 publications

Liposomal Drug Delivery: A Versatile Platform for Challenging Clinical Applications

Liposomal Drug Delivery: A Versatile Platform for Challenging Clinical Applications

Sugar-based novel niosomal nanocarrier system for enhanced oral bioavailability of levofloxacin

Efficient Delivery and Functional Expression of Transfected Modified mRNA in Human Embryonic Stem Cell-derived Retinal Pigmented Epithelial Cells

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