A Novel Cell Therapy Method for Recovering after Brain Stroke in Rats

Abstract

BackgroundNowadays, stroke leads to a significant part of the adult mortality and morbidity and also it could result in some neurological deficits in the patients’ lives. Cell therapy has opened a new approach to treat the brain ischemia and reduce its terrible effects on the patients’ lives. There are several articles which show that the cell therapy could be beneficial for treating brain stroke. In this study, we have planned to present a new cell therapy method for stroke by administration of Mesenchymal stem cells and differentiated neural stem cells without astrocytes.Method and MaterialsThe Mesenchymal stem cells were isolated from tibia and femur of a 250~300 g rat and they were cultured in DMEM/F12, 10% fetal bovine serum, 1% Pen/Strep. Neural stem cells were isolated from 14 days rat embryo ganglion eminence and were cultured in NSA media containing Neurobasal, 2% B27, bFGF 10 ng/ml and EGF 20 ng/ml after 5 days they formed some neurospheres. The isolated neural stem cells were differentiated to neural lineages by adding 5% fetal bovine serum to their culture media. After 48 hours the astrocytes were depleted by using MACS kit.ResultsThe group that received Mesenchymal stem cells systemically and differentiated neural stem cells without astrocytes had the best neurological outcomes and the least infarct volume and apoptosis. It could be understood that this cell therapy method might cause almost full recovery after brain stoke.ConclusionUsing combination cell therapy with Mesenchymal stem cells and differentiated neural stem cells with removed astrocyte could provide a novel method for curing brain stroke.

Optimization of time for neural stem cells transplantation for brain stroke in rats

“…Noticeable amongst these are neural stem cells (3,4), bone marrow stem cells (3,5,6), cord blood derived MSCs (7,8), adipose tissue derived (5), embryonic stem cells and their derivatives (9), and induced pluripotent stem cells (iPSCs) (10,11). Nevertheless, ease of availability without moral and ethical strings and differentiation capacity to adopt desired phenotype are vital issues for the search of ideal donor cells for transplantation therapy.…”

Optimized Protocol is the Key for Clinical Use of Stem Cell Therapy in Stroke

“…Astrocytes secrete inflammatory cytokines [28]; therefore, they might be an obstacle for achieving the optimum effect of NSCs transplantation. By omitting the astrocytes, the efficacy of NSCs transplantation would be increased [29].…”