A Novel Circular RNA circITSN2 Targets the miR-218-5p/LMO7 Axis to Promote Chicken Embryonic Myoblast Proliferation and Differentiation

Shen, Xiaoxu; Wei, Yuanhang; Liu, Wei; You, Guishuang; Tang, Shuyue; Su, Zhenyu; Du, Mingxin; He, Jian; Zhao, Jing; Tian, Yongtong; Zhang, Yao; Ma, Menggen; Zhu, Qing; Yin, Huadong

doi:10.3389/fcell.2021.748844

Cited by 15 publications

(10 citation statements)

References 42 publications

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“…S4A). Lmo7 was reported to be required for proper myoblast differentiation by temporally regulating myogenic differentiation genes ( 28 ), and Foxp1 was shown to be required for neuronal differentiation in the neocortex and the hippocampus ( 29 ). Mutations in Foxp1 are causative for neurodevelopment disorders such as autism spectrum disorders ( 30 ).…”

Section: Resultsmentioning

confidence: 99%

FOXG1 drives transcriptomic networks to specify principal neuron subtypes during the development of the medial pallium

Yang

Zhang

et al. 2023

Sci. Adv.

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The medial pallium (MP) is the major forebrain region underlying learning and memory, spatial navigation, and emotion; however, the mechanisms underlying the specification of its principal neuron subtypes remain largely unexplored. Here, by postmitotic deletion of FOXG1 (a transcription factor linked to autism spectrum disorders and FOXG1 syndrome) and single-cell RNA sequencing of E17.5 MP in mice, we found that FOXG1 controls the specification of upper-layer retrosplenial cortical pyramidal neurons [RSC-PyNs (UL)], subiculum PyNs (SubC-PyNs), CA1-PyNs, CA3-PyNs, and dentate gyrus granule cells (DG-GCs) in the MP. We uncovered subtype-specific and subtype-shared FOXG1-regulated transcriptomic networks orchestrating MP neuron specification. We further demonstrated that FOXG1 transcriptionally represses Zbtb20 , Prox1 , and Epha4 to prevent CA3-PyN and DG-GC identities during the specification of RSC-PyNs (UL) and SubC-PyNs; FOXG1 directly activates Nr4a2 to promote SubC-PyN identity. We showed that TBR1, controlled by FOXG1 during CA1-PyN specification, was down-regulated. Thus, our study illuminates MP principal neuron subtype specification and related neuropathogenesis.

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Section: Resultsmentioning

confidence: 99%

FOXG1 drives transcriptomic networks to specify principal neuron subtypes during the development of the medial pallium

Yang

Zhang

et al. 2023

Sci. Adv.

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“…LMO7 is essential for skeletal muscle development and encodes a transcription factor that binds to the PAX3 , MyoD , and Myf5 promoters to activate expression of key myogenic differentiation genes in C2C12 cells [ 64 ]. Thus, downregulation of LMO7 was shown to inhibit myogenesis in chicken myoblasts [ 36 ]. In addition, circLMO7 (formed by circularization of exons 3–5 of bovine LMO7 ), can target miR-378a-3p to inhibit the differentiation and apoptosis of myoblasts, and the sequence of bovine circLMO7 is completely different from that of porcine circ_0016243 and circ_0016253 in our study [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of circular RNA-mediated ceRNA regulation in porcine skeletal muscle development

et al. 2023

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Background As a diverse and abundant class of endogenous RNAs, circular RNAs (circRNAs) participate in various biological processes including cell proliferation and apoptosis. Nevertheless, few researchers have investigated the role of circRNAs in muscle development in cultivated pigs. Results In this study, we used RNA-seq to construct circRNA expression profiles in skeletal muscle of Jinfen White pigs at the age of 1, 90, and 180 days. Among the 16,990 identified circRNAs, 584 circRNAs were differentially expressed. Moreover, the enrichment analysis of DE circRNA host genes showed that they were mainly involved in muscle contraction, muscle organ development and muscle system processes, as well as AMPK and cAMP-related signal pathways. We also constructed a circRNA–miRNA–mRNA co-expression network to find key circRNAs which many involved in the regulation of porcine skeletal muscle development through the competitive endogenous RNA (ceRNA) mechanism. It is noteworthy that circ_0018595/miR-1343/PGM1 axis may play a regulatory role in the development of porcine skeletal muscle. Conclusions This study identified the circRNAs and present the circRNA expression profile in the development of pigs, revealed that DE circRNA host genes participate in different cell fates and enriched the porcine ceRNA network. Thus, this work will become a valuable resource for further in-depth study of the regulatory mechanism of circRNA in the development of porcine skeletal muscle.

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“…LMO7 is essential for skeletal muscle development and encodes a transcription factor that binds to the PAX3, MyoD, and Myf5 promoters to activate expression of key myogenic differentiation genes in C2C12 cells [60]. Thus, downregulation of LMO7 was shown to inhibit myogenesis in chicken myoblasts [37]. In addition, circLMO7 (formed by circularization of exons 3-5 of bovine LMO7), can target miR-378a-3p to inhibit the differentiation and apoptosis of myoblasts, and the sequence of bovine circLMO7 is completely different from that of porcine novel_circ_0016243 and novel_circ_0016253 in our study [61].…”

Section: Discussionmentioning

confidence: 99%

“…The existing research on circRNAs in muscle development is far from su cient, and their regulatory mechanisms have not yet been fully clari ed. Thus, further exploration is needed [36][37][38].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of Circular RNA-Mediated ceRNA Regulation in Porcine Skeletal Muscle Development

Yun

Huang

Liu

et al. 2022

Preprint

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Background: As a diverse and abundant class of endogenous RNAs, circular RNAs (circRNAs) participate in various biological processes including cell proliferation and apoptosis. Nevertheless, few researchers have investigated the role of circRNAs in muscle development in cultivated pigs. Results: In this study, we used RNA-seq to construct circRNA expression profiles in skeletal muscle of Jinfen White pigs at the age of 1, 90, and 180 days. Among the 16,990 identified circRNAs, 584 were differentially expressed, with 255, 477, and 63 DE circRNAs in the 90 d vs. 1 d, 180 d vs. 1 d, and 180 d vs. 90 d groups, respectively. Moreover, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of DE circRNA host genes revealed them to be mainly involved in skeletal muscle fiber-related processes (e.g., muscle contraction, muscle organ development, and muscle system processes) and skeletal muscle fiber-related signaling pathways (e.g., AMPK and cAMP pathways). We also constructed circRNA–miRNA–mRNA co-expression network to screen out circRNAs many involved in the regulation of porcine skeletal muscle growth and development through the competitive endogenous RNA (ceRNA) mechanism. In this network, we predicted circ_0018595 may as a potential sponge of miR-1343 to regulate PGM1 expression, in turn promoting the proliferation of pig skeletal muscle satellite cells. The structure and expression of circ_0018595 were confirmed using convergent and divergent primer amplification, RNase R digestion, and qRT-PCR. Conclusions: This study has identified 584 candidate circRNAs, especially circ_0018595, which may be involved in the growth and development of porcine skeletal muscle, and will therefore serve as a valuable resource for further in-depth study of circRNA regulatory mechanisms in skeletal muscle development.

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A Novel Circular RNA circITSN2 Targets the miR-218-5p/LMO7 Axis to Promote Chicken Embryonic Myoblast Proliferation and Differentiation

Cited by 15 publications

References 42 publications

FOXG1 drives transcriptomic networks to specify principal neuron subtypes during the development of the medial pallium

FOXG1 drives transcriptomic networks to specify principal neuron subtypes during the development of the medial pallium

Genome-wide analysis of circular RNA-mediated ceRNA regulation in porcine skeletal muscle development

Genome-Wide Analysis of Circular RNA-Mediated ceRNA Regulation in Porcine Skeletal Muscle Development

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