A novel classification of tumour budding in colorectal cancer based on the presence of cytoplasmic pseudo‐fragments around budding foci

Shinto, Eiji; Mochizuki, Hidetaka; Ueno, Hideki; Matsubara, Osamu; Jass, Jeremy R.

doi:10.1111/j.1365-2559.2005.02162.x

Cited by 93 publications

(116 citation statements)

References 17 publications

Supporting

Mentioning

108

Contrasting

Unclassified

Order By: Relevance

“…Tumor budding is defined as the presence of isolated single cells or small cell clusters (up to 4) scattered in the stroma at the invasive tumor margin and is established as an adverse prognostic indicator. [28][29][30][31][32][33] Tumor budding is at least in part driven by the wnt signaling pathway as attested by the fact that nuclear b-catenin accumulates in the nuclei in tumor buds (dedifferentiated cancer cells) at the invasive tumor border. [34][35][36][37] In the present study, nuclear pERK expression was correlated with increasing RHAMM expression in MMR proficient (P ¼ 0.012) and with complete RHAMM expression in presumed Lynch syndrome (P ¼ 0.03), whereas an association was not found in MLH1-negative CRC.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of the receptor for hyaluronic acid mediated motility is an independent adverse prognostic factor in colorectal cancer

et al. 2006

View full text Add to dashboard Cite

RHAMM, a member of the microtubule-associated protein family that interacts with the mitogen-activated protein kinase pathway, is associated with tumor progression, aggressive disease and shortened survival in several tumor types. This study aimed to determine the prognostic value of RHAMM in colorectal cancer (CRC). A series of 1420 unselected, nonconsecutive CRC resections were subdivided into three groups: (1) DNA mismatch repair (MMR)-proficient, (2) MLH1 negative and (3) presumed Lynch syndrome. Immunohistochemical analysis of RHAMM expression (0 vs 40%), increasing expression (increasing percentage positivity) and complete expression (100 vs o100%) was performed using tissue microarray technique and the results were correlated with clinicopathological parameters. Fifty-seven tissue samples of normal colonic mucosa were included as a control group. In a univariate analysis increasing and complete expression of RHAMM were associated with higher N stage (P ¼ 0.023 and 0.021) and worse survival (Po0.0001) in MMR-proficient CRC. Complete expression of RHAMM was associated with worse survival in presumed Lynch syndrome (P ¼ 0.016). In MLH1-negative CRC there was no association between RHAMM expression and the clinicopathological features. In a multivariate analysis, increasing RHAMM expression was an independent adverse prognostic factor in MMR-proficient CRC (Po0.0001) and complete expression in MMR-proficient CRC and presumed Lynch syndrome (Po0.0001 and P ¼ 0.031, respectively). Nuclear pERK expression was associated with increasing RHAMM expression in MMR-proficient CRC (P ¼ 0.012) and with complete RHAMM expression in presumed HNPCC (P ¼ 0.03). Increasing and complete RHAMM expressions are independent adverse prognostic factors in MMR-proficient CRC and presumed Lynch syndrome.

show abstract

Section: Discussionmentioning

confidence: 99%

Overexpression of the receptor for hyaluronic acid mediated motility is an independent adverse prognostic factor in colorectal cancer

et al. 2006

View full text Add to dashboard Cite

show abstract

“…They identified a subset of colorectal carcinomas with invasive fronts that displayed a strikingly different architecture compared with the central area of the tumor, with well-developed glandular structures giving way to isolated tumor cells and small cell groups, as illustrated in Figure 1. At the ultrastructural level, Shinto et al 12,13 used immunohistochemistry with anti-cytokeratin antibodies to highlight another feature of buds, termed cytoplasmic pseudofragmentation, whereby cytoplasmic fragments, visible only by immunohistochemistry, are seen in the immediate vicinity of tumor buds. When examined on serial sections, some of the fragments were shown to be connected to the buds (hence the term 'pseudofragments').…”

Section: Morphological Featuresmentioning

confidence: 99%

“…12,13,22,23,31,32,38,40,43,44,48,51,54,55 The use of immunohistochemistry in the assessment of budding is somewhat controversial: some authors argue that the evaluation of budding should be limited to H&E-stained slides because of the cost and impracticality of performing immunohistochemistry in routine cases, while others argue that immunohistochemistry should be used routinely to improve the accuracy and reproducibility of bud counts. In addition to cost considerations, the use of immunohistochemistry may also require the establishment of a separate cutoff for defining positive budding.…”

Section: Role Of Immunohistochemistry In the Evaluation Of Buddingmentioning

confidence: 99%

Tumor budding in colorectal carcinoma: time to take notice

et al. 2012

View full text Add to dashboard Cite

“…In colorectal carcinomas, 'tumor budding' has been defined as the presence of isolated single neoplastic cells or small cell clusters of up to four cells in the stroma at the invasive tumor margin (1)(2)(3)(4)(5)(6)(7)(8). It was first reported by Imai (9).…”

Section: Introductionmentioning

confidence: 99%

“…There are reports which determines the association of tumor budding at the invasive tumor margin and tumor behavior (3)(4)(5)(6)(7)(8). Dedifferentiation and dissociation of cancer cells has been reported to be the first event of invasion and metastasis in experimental studies.…”

Section: Introductionmentioning

confidence: 99%

Tumor budding in colorectal carcinomas

Bektas

Mamak²,

Çiriş³

et al. 2012

TJPATH

View full text Add to dashboard Cite

Objective: In colorectal carcinomas, tumor budding has been defined as the presence of isolated single tumor cells or small cell clusters in the stroma at the invasive tumor margin. In this study, the relationship between tumor budding density at the invasive tumor margin and pathological parameters is investigated. Material and Method:Haematoxylin and eosin stained slides of 73 cases with colorectal carcinoma were retrospectively evaluated for the presence and intensity of tumor budding by 2 observers. After the specimens were assessed, the highest density of tumor budding area was counted in a microscopic field of x200. Cases were separated into 2 groups according to tumor budding density as low grade (<10) and high grade (≥10). The relationship of these groups with depth of tumor invasion, histological grade, vascular invasion and lymph node involvement was investigated. Results:Of the 73 colorectal carcinoma cases, 33 (45.2%) had low and 40 (54.8%) had high grade tumor budding density, respectively. There was a statistically significant relationship between high grade tumor budding density and histological grade (p=0.042), lymph node involvement (p=0.0001) and vascular invasion (p=0.0034). Conclusion:High grade tumor budding density is associated with aggressive phenotypical features in colorectal carcinoma.Key Words: Colorectal neoplasms, Neoplasm invasion, Lymph node, Metastasis ÖZ Amaç: Kolorektal karsinomlarda tümör tomurcuklanması, invaziv tümör komşuluğundaki stromada bulunan izole tümör hücreleri ya da küçük tümöral hücre kümeleri olarak tanımlanmaktadır. Bu çalışmada kolorektal karsinomlarda invaziv tümör komşuluğundaki stromada bulunan tümör tomurcuklanması yoğunluğunun patolojik özelliklerle ilişkisi araştırılmıştır. Gereç ve Yöntem:Retrospektif olarak, kolorektal karsinomlu 73 olguya ait hematoksilen-eozin boyalı preparatlar tümör tomurcuklanması varlığı ve yoğunluğunun tespit edilmesi amacıyla 2 gözlemci tarafından değerlendirilmiştir. Preparatlar tekrar değerlendirildikten sonra tümör tomurcuklanmaları, en yoğun oldukları alan tespit edilerek x200 büyütme alanında sayılmıştır. Olgular, tümör tomurcuklanması yoğunluğu 10' dan az olanlar düşük dereceli, 10 ve üzeri olanlar yüksek dereceli olmak üzere 2 gruba ayrılmıştır. Bu grupların tümörün invazyon derinliği, histolojik derecesi, lenf nodu tutulumu ve vasküler invazyon gibi patolojik özelliklerle ilişkisi araştırılmıştır. Bulgular: Kolorektal karsinomlu 73 olgunun 33'ünde (%45,2) düşük, 40'ında (%54,8) yüksek dereceli tümör tomurcuklanması yoğunluğu tespit edilmiştir. Yüksek dereceli tümör tomurcuklanması yoğunluğu ile histolojik derece (p=0.042), lenf nodu tutulumu (p=0,0001) ve vasküler invazyon (p=0,0034) arasında istatistiksel olarak anlamlı ilişki saptanmıştır.Sonuç: Yüksek dereceli tümör tomurcuklanması yoğunluğu kolorektal karsinomlarda agresif fenotipik özelliklerle ilişkilidir.

show abstract

A novel classification of tumour budding in colorectal cancer based on the presence of cytoplasmic pseudo‐fragments around budding foci

Abstract: Cytoplasmic pseudo-fragments may be a marker for an activated budding phenotype that is associated with cell motility and increased invasiveness in CRC and is independent of the extent of budding.

Cited by 93 publications

References 17 publications

Overexpression of the receptor for hyaluronic acid mediated motility is an independent adverse prognostic factor in colorectal cancer

Overexpression of the receptor for hyaluronic acid mediated motility is an independent adverse prognostic factor in colorectal cancer

Tumor budding in colorectal carcinoma: time to take notice

Tumor budding in colorectal carcinomas

Contact Info

Product

Resources

About