A novel colorimetric sensor using aptamers to enhance peroxidase-like property of gold nanoclusters for detection of Escherichia coli O157:H7 in milk

Yang, Song; Wang, Lihan; Zhao, Jiayuan; Li, Hongxuan; Yang, Xinyan; Fu, Shiqian; Xue, Qiang; Qing, Chen; Jiang, Yujun; Man, Chaoxin

doi:10.1016/j.idairyj.2022.105318

Cited by 24 publications

(9 citation statements)

References 38 publications

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“…coli O157:H7 in ultrahigh temperature (UHT) sterilized, pasteurized, and raw milk. The prediscussed aptamer sequence was 5′-SH–CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG-3′ . In another work, Q. Chen et al developed aptamers@BSA-AuNCs for the selective detection of Salmonella typhimurium ( S. typhimurium ) using dual thiolated aptamers, BSA-AuNCs, and the TMB/H 2 O 2 system.…”

Section: Capping Agents For Colorimetric Assays Based On Fluorescent ...mentioning

confidence: 99%

Challenges and Opportunities of Using Fluorescent Metal Nanocluster-Based Colorimetric Assays in Medicine

Mohseni,

Moodi,

Kefayat

et al. 2024

ACS Omega

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Development of rapid colorimetric methods based on novel opticalactive metal nanomaterials has provided methods for the detection of ions, biomarkers, cancers, etc. Fluorescent metal nanoclusters (FMNCs) have gained a lot of attention due to their unique physical, chemical, and optical properties providing numerous applications from rapid and sensitive detection to cellular imaging. However, because of very small color changes, their colorimetric applications for developing rapid tests based on the naked eye or simple UV−vis absorption spectrophotometry are still limited. FMNCs with peroxidase-like activity have significant potential in a wide variety of applications, especially for point-of-care diagnostics. In this review, the effect of using various capping agents and metals for the preparation of nanoclusters in their colorimetric sensing properties is explored, and the synthesis and detection mechanisms and the recent advances in their application for ultrasensitive chemical and biological analysis regarding human health are highlighted. Finally, the challenges that remain as well as the future perspectives are briefly discussed. Overcoming these limitations will allow us to expand the nanocluster's application for colorimetric diagnostic purposes in medical practice.

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Section: Capping Agents For Colorimetric Assays Based On Fluorescent ...mentioning

confidence: 99%

Challenges and Opportunities of Using Fluorescent Metal Nanocluster-Based Colorimetric Assays in Medicine

Mohseni,

Moodi,

Kefayat

et al. 2024

ACS Omega

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“…Compared to antibodies, aptamers offer the advantages of stability, low cost, and ease of synthesis. Therefore, Song et al (2022) proposed a colorimetric nanosensor based on aptamers and papain-stabilized AuNCs (papain-AuNCs) for detecting E. coli O157:H7. E. coli O157:H7 binds to aptamer-modified papain-AuNCs, leading to the inhibition of the peroxidase activity of papain-AuNCs.…”

Section: Foodborne Pathogens Detectionmentioning

confidence: 99%

“…Foodborne pathogens, a major cause of such illnesses, can contaminate food at various stages, leading to outbreaks and recalls (Chen, He, et al, 2021;Riley, 2020). Escherichia coli O157:H7 is a common foodborne pathogen found in dairy products and can cause severe health issues (Hiremath et al, 2021;Song et al, 2022). Wang, Xing et al (2019) developed a fluorescence sensing method based on DNA-AgNCs for detecting E. coli O157:H7 in milk.…”

Section: Foodborne Pathogens Detectionmentioning

confidence: 99%

Optical nanosensors based on noble metal nanoclusters for detecting food contaminants: A review

Pang,

Pi,

Zhao

et al. 2024

Comp Rev Food Sci Food Safe

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Food contaminants present a significant threat to public health. In response to escalating global concerns regarding food safety, there is a growing demand for straightforward, rapid, and sensitive detection technologies. Noble metal nanoclusters (NMNCs) have garnered considerable attention due to their superior attributes compared to other optical materials. These attributes include high catalytic activity, excellent biocompatibility, and outstanding photoluminescence properties. These features render NMNCs promising candidates for crafting nanosensors for food contaminant detection, offering the potential for the development of uncomplicated, swift, sensitive, user‐friendly, and cost‐effective detection approaches. This review investigates optical nanosensors based on NMNCs, including the synthesis methodologies of NMNCs, sensing strategies, and their applications in detecting food contaminants. Furthermore, it involves a comparative assessment of the applications of NMNCs in optical sensing and their performance. Ultimately, this paper imparts fresh perspectives on the forthcoming challenges. Hitherto, optical (particularly fluorescent) nanosensors founded on NMNCs have demonstrated exceptional sensing capabilities in the realm of food contaminant detection. To enhance sensing performance, future research should prioritize atomically precise NMNCs synthesis, augmentation of catalytic activity and optical properties, development of high‐throughput and multimode sensing, integration of NMNCs with microfluidic devices, and the optimization of NMNCs storage, shelf life, and transportation conditions.

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“…127 Aptamer-modified papain–AuNCs with POD-like activity were used for the detection of Escherichia coli O157:H7. 128…”

Section: The Roles Played By Auncs Stabilized By Templates Consisting...mentioning

confidence: 99%

The roles of templates consisting of amino acids in the synthesis and application of gold nanoclusters

Ma,

Yang,

Zhang

et al. 2024

Nanoscale

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A novel colorimetric sensor using aptamers to enhance peroxidase-like property of gold nanoclusters for detection of Escherichia coli O157:H7 in milk

Cited by 24 publications

References 38 publications

Challenges and Opportunities of Using Fluorescent Metal Nanocluster-Based Colorimetric Assays in Medicine

Challenges and Opportunities of Using Fluorescent Metal Nanocluster-Based Colorimetric Assays in Medicine

Optical nanosensors based on noble metal nanoclusters for detecting food contaminants: A review

The roles of templates consisting of amino acids in the synthesis and application of gold nanoclusters

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