A Novel Combination of Wheat Peptides and Fucoidan Attenuates Ethanol-Induced Gastric Mucosal Damage through Anti-Oxidant, Anti-Inflammatory, and Pro-Survival Mechanisms

Abstract

Gastritis or peptic ulcer is believed to affect about half of people worldwide. Traditional medications can lead to adverse effects, therefore, alternative nutritional strategies are needed to prevent the development of gastric mucosal damage. A novel combination of two food-grade ingredients, wheat peptides and fucoidan (WPF), was prepared to treat male Sprague Dawley rats for 30 days before gastric mucosal damage was induced by oral administration of ethanol. The serum levels of biomarkers were determined by enzyme-linked immunosorbent assay. Biomarkers in stomach tissue were analyzed using immunohistochemistry. In addition, human gastric epithelial cell line (GES-1) was used to investigate protein expression by Western blot. WPF could attenuate ethanol-induced gastric mucosal damage in an inverse dose-dependent manner, with both ulcer index and pathological index improved. WPF increased superoxide dismutase level and decreased malondialdehyde level. WPF also decreased the levels of interleukin-8, platelet-activating factor, and Caspase 3, while increasing the levels of prostaglandin E-2, epidermal growth factor (EGF), and EGF receptor (EGFR). Furthermore, phosphorylation of EGFR and extracellular signal–regulated kinases was induced by WPF in GES-1 cells. In conclusion, the novel combination of wheat peptides and fucoidan attenuated ethanol-induced gastric mucosal damage in rats through anti-oxidant, anti-inflammatory, and pro-survival mechanisms.

A New Participant in the Pathogenesis of Alcoholic Gastritis: Pyroptosis

“…Increased levels of inflammation with the release of large amounts of cytokines are the main features of gastritis [50][51][52][53]. It has become clear that bacteria have caused gastritis in the overwhelming majority of the cases recorded since 1982 [54].…”

<p>Selenium Nanoparticles-Embedded Chitosan Microspheres and Their Effects Upon Alcohol-Induced Gastric Mucosal Injury in Rats: Rapid Preparation, Oral Delivery, and Gastroprotective Potential of Selenium Nanoparticles</p>

“…Gross mucosal lesions were recognized as hemorrhage spot or damage strip (erosions) on the mucosal surface. 6 The areas of stomach tissue and gross lesions were approximately measured by using Image J (v1.48) software (developed by National Institutes of Health, USA). 28 The results were expressed as the gross ulcer index-1 (GUI-1) calculated by using Equation 2.…”

“…Chronic gastric ulcer is closely associated with smoking, bacteria infection, irregular eating habits, chronic bile reflux, autoimmune disorders and stress, whereas acute gastric ulcer is usually caused by excessive consumption of alcohol or high dose of non-steroidal anti-inflammatory drugs (NSAIDs). [1][2][3][4][5][6] High concentration of alcohol (>40%, v/v) and overconsumption of alcoholic beverages can lead to acute gastric ulcers characterized by powerful oxidative stress. [1][2][3][4][6][7][8] Selenium (Se) is an indispensable trace element required for most of the living organisms including animals and human beings.…”

“…[1][2][3][4][5][6] High concentration of alcohol (>40%, v/v) and overconsumption of alcoholic beverages can lead to acute gastric ulcers characterized by powerful oxidative stress. [1][2][3][4][6][7][8] Selenium (Se) is an indispensable trace element required for most of the living organisms including animals and human beings. 9 Se-deficiency might condone the development of acute gastric mucosal injury induced by alcohol, especially for those living in Se-deficient area.…”

Влияние Глицилпролина На Показатели Стимулированной Карбахолом Желудочной Секреции У Крыс В Условиях Многокомпонентного Стресса

“…Âåäóùåå ìåñòî â ðåàêöèè àäàïòàöèè íà ñòðåññ è íàðóøåíèè ãîìåîñòàçà ñî ñòîðîíû aeåëóäêà çàíèìàþò ïåïòèäû, ÷òî ïðîäåìîíñòðèðîâàíî â ýêñïåðèìåíòàõ íà ðàçíûõ ìîäåëÿõ ñòðåññà [8, 13,17]. Çíà÷èòåëüíóþ ðîëü â ðåãóëÿöèè ñåêðåòîðíîé àêòèâíîñòè aeåëóäêà èãðàþò âûñîêîñòàáèëüíûå â ñðåäàõ êîðîòêèå ïåïòèäû -ãëèïðîëèíû (Pro-Gly-Pro, Pro-Gly, Gly-Pro è äð.…”