A novel combined analytical UV and MS approach for the quantification of oleuropein metabolites in human biological samples when authentic standards are not available

Mitochondrial Calcium Uptake Declines during Aging and is Directly Activated by Oleuropein to Boost Energy Metabolism and Skeletal Muscle Performance

“…5A and 5B). In a 2 to 8 hour time course after acute oral intake, OLE induced a sustained 36-58% dephosphorylation of PDH in skeletal muscle that was already prominent after 2h, consistent with the reported rapid peak of oleuropein and its metabolites in blood (Garcia-Villalba et al, 2014;Polia et al, 2022) (Fig 5C and S7A). As expected, dephosphorylation was mirrored by increased PDH activity in skeletal muscle which was boosted by +44% after acute OLE intake (Fig.…”

“…Animals were treated orally with an Oleuropein-rich olive leaf extract (OLE) standardized with 40% of Oleuropein as previously reported (Polia et al, 2022). Acute single dose administration was performed by an oral gavage of 50mg/kg OLE dissolved in water, or vehicle, and skeletal muscle was collected after euthanasia 2 to 8h post gavage.…”