A novel conducting polymer based platform for ethanol sensing

Kekec, Nur Cicek; Kanik, Fulya Ekiz; Udum, Yasemin Arslan; Hızlıateş, Cevher Gündoğdu; Ergün, Yavuz; Toppare, Levent

doi:10.1016/j.snb.2013.12.007

Cited by 25 publications

(14 citation statements)

References 28 publications

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“…In bioactive platform preparation, EDC and NHS were used as cross linkers to obtain a covalent binding between amino groups of the polymer and carboxylic acid groups of the enzyme molecules [15][16][17]. The enzyme solution that contained 3 µL (3.33 U) AO, 3 µL 0.4 M EDC and 3 µL 0.1 M NHS was dip-coated on the surface of the polymer coated electrode [18]. The modified electrodes were stored in a refrigerator (4 °C).…”

Section: B Fabrication Of Biosensormentioning

confidence: 99%

Fabrication of Chiral Poly(3,4-ethylenedioxythiophene) Derivatives Modified Glassy Carbon Electrodes for Ascorbic Acid Determination

Dong¹,

Hu²,

Sun³

et al. 2016

Proceedings of the 2015 International Conference on Materials Chemistry and Environmental Protection (Meep-15)

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Two chiral poly(N-(tert-butoxycarbonyl)-Lphenylalayl (3,4-ethylenedioxythiophene-2'-yl)methylamide) (PEDOT-Boc-L-Phe) and poly(L-phenylalayl (3,4ethylenedioxythiophene-2'-yl)methylamide) (PEDOT-L-Phe) were synthesized by electrochemical polymerization, and their properties as an immobilization platform for biosensor application were explored. After covalent immobilization of ascorbate oxidase (AO) on the polymeric matrixs, their applications for ascorbic acid (AA) biosensing were investigated in detail. Importantly, optimized biosensors showed very good linear relationships between [AA] and the steady-state current response (I) in the concentration range of 0.03 μM to 7 mM (y = 0.08972 x ＋ 0.5785, r = 0.9954, (PEDOT-Boc-Phe)) and 0.07 μM to 7 mM (y = 0.0397x + 0.59381, r = 0.9928 (PEDOT-Phe)), respectively. Satisfactory results implied that the as-formed polymer films were realized the immobilization of biologically active species and could be candidates in the development of biosensing performance.

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Section: B Fabrication Of Biosensormentioning

confidence: 99%

Fabrication of Chiral Poly(3,4-ethylenedioxythiophene) Derivatives Modified Glassy Carbon Electrodes for Ascorbic Acid Determination

Dong¹,

Hu²,

Sun³

et al. 2016

Proceedings of the 2015 International Conference on Materials Chemistry and Environmental Protection (Meep-15)

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“…Alcohol oxidase (AOx) from Pichia pastoris [1][2][3][4][5][6][7], Hansenula polymorpha [8][9][10] and Candida boidinii sp. [11], as a bio-recognition element for ethanol sensing [1][2][3][7][8][9][10][11] and development of biofuel cells [4][5][6], has been the focal point of many studies in the last years.…”

Section: Introductionmentioning

confidence: 99%

“…[11], as a bio-recognition element for ethanol sensing [1][2][3][7][8][9][10][11] and development of biofuel cells [4][5][6], has been the focal point of many studies in the last years. It is oligomeric enzyme consisting of eight identical subunits, each containing a tightly bound flavin adenine dinucleotide (FAD) redox center [12].…”

Section: Introductionmentioning

confidence: 99%

Bioelectrocatalytic Activity of Immobilized Alcohol Oxidase on 4‐(pyrrole‐1‐yl) Benzoic Acid Modified Carbon Nanotubes

Kowalewska

Jakubow

2016

Electroanalysis

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A novel bioelectrocatalytic system was prepared by immobilizing alcohol oxidase (AOx) onto multiwalled carbon nanotubes (MWCNT) modified with 4‐(pyrrole‐1‐yl) benzoic acid (PyBA). Functional carboxylic groups from PyBA create covalent amide linkages with amine groups from the enzyme molecule and provide an anchor for the effective immobilization of AOx improving the stability of the whole system. The immobilized enzyme displayed a pair of reversible redox peaks of flavin adenine dinucleotide (FAD) cofactor with the formal potential E0’=−0.451 V. The response showed a surface‐controlled electrode process with the heterogeneous electron transfer rate constant ks=2.7 s−1. Under aerobic conditions AOx(FADH2) can be oxidized to AOx(FAD) by oxygen, which then reacts with ethanol decreasing the cathodic response, which could be used for ethanol detection with a high sensitivity 13.1 μA mM−1 cm−2. The lack of bioactivity towards ethanol in anaerobic conditions suggests the presence of two types of AOx molecules in the system: active with oxygen maintaining the direct electron transfer feature and not active without a redox mediator, due to the deeply embedded FAD cofactor. The polarization curve showed that the electrooxidation current of ethanol appears at −410 mV and reaches 2.0 µA cm−1 at −300 mV. In this case, the bioactivity of AOx to ethanol can be observed offering promising solution for the development of mediatorless systems for application to biosensors and biofuel cells.

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“…In addition, alcohol are widely used in the clinical analysis and food industry. Especially, it is crucial to monitor ethanol during fermentation processes in biotechnological production of beverages to ensure food quality and safety [20]. Thus, it is necessary to have a cost-effective, simple, and convenient method for the detection of benzaldehyde, benzyl alcohol and ethanol.…”

Section: Introductionmentioning

confidence: 99%

A multifunctional chemical sensor based on a three-dimensional lanthanide metal-organic framework

Liao

et al. 2016

Journal of Solid State Chemistry

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A novel conducting polymer based platform for ethanol sensing

Cited by 25 publications

References 28 publications

Fabrication of Chiral Poly(3,4-ethylenedioxythiophene) Derivatives Modified Glassy Carbon Electrodes for Ascorbic Acid Determination

Fabrication of Chiral Poly(3,4-ethylenedioxythiophene) Derivatives Modified Glassy Carbon Electrodes for Ascorbic Acid Determination

Bioelectrocatalytic Activity of Immobilized Alcohol Oxidase on 4‐(pyrrole‐1‐yl) Benzoic Acid Modified Carbon Nanotubes

A multifunctional chemical sensor based on a three-dimensional lanthanide metal-organic framework

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