A novel copper ion sensing fluorescent probe for fast detection of pyrophosphate and alkaline phosphatase

Abstract

A Cu2+ sensing fluorescent probe is synthesized via a Mannich reaction and is applied in the fluorescence detection of pyrophosphate and alkaline phosphatase.

Polyacrylic Acid-Ca2+(Ce3+) Nanoclusters as Fluorescent “Off–On–Off” Probes for Quantitative Detection of Pyrophosphate and Alkaline Phosphatase

“…Recently, several fluorescence strategies have been developed to detect PPi and ALP. For example, Chen’s group synthesized Cu 2+ fluorescent probe using 4-hydroxyhypoxidone, paraformaldehyde, and proline, and this probe is capable of detecting both PPi and ALP . Chai’s group designed carbon quantum dots-based reversible fluorescence assay to detect PPi, based on the disaggregating effect of PPi on the Ce 3+ , inducing severe aggregation of surface carboxylated CQDs; moreover, this probe was further utilized to detect ALP in the presence of ATP. , In addition, based on the outstanding optical properties of rare earth elements, including large stoke shifts, sharp emission bands, long lifetime, and chemical stability, − the fluorescent strategies for quantitative detection of PPi and ALP have been developed.…”

“…Hitherto, a variety of methods have been applied in the quantitative detection of PPi, including fluorescence, , colorimetry, , and chemiluminescence . On the other hand, fluorescent strategies, , colorimetric methods, , electrochemistry, , chemiluminescence, , and surface-enhanced Raman scattering have also been developed for qualitative and quantitative detection of ALP. Compared to colorimetric methods with weak anti-interference capability and high detection limits, and electrochemical analysis methods with relatively weak signals and low sensitivity, fluorescence methods feature low sample requirements and strong resistance to interference.…”