A Novel Cost-Effective Nanobody against Fumonisin B1 Contaminations: Efficacy Test in Dairy Milk and Chickens

Abstract

Background: Fumonisin B1 (FB1) is a secondary metabolite produced mainly by Fusarium verticillioides or Fusarium proliferatum. It poses a huge threat to the sustainable animal industry and human health as well via food chains (egg, meat and milk). Although E. coli-expressed nanobodies are documented for diagnostic applications, nanobodies remain elusive as FB1 detoxifiers in feed and food. Results: In the present study, the E. coli-expressed nanobody was assessed to remove FB1 in fresh milk, embryonated eggs and broilers. Firstly, 2 alpacas received intramuscularly FB1-adjuvanted BSA 6 times, and then the variable domain of the heavy-chain antibody (VHH) of fb1 genes were amplified to clone into the pCANTAB 5 E vector in order to generate a VHH-FB1 phage antibody display library, yielding 3.4 × 1010 capacity with 96.7% positivity. Afterwards, 5 anti-FB1 nanobodies were expressed and identified. Furthermore, maximal 43.2% FB1 was removed from milk by 1:2000 concentration of nanobody 5 (Nb5). Furthermore, SPF-embryonated eggs were inoculated into albumens with nanobody-treated FB1. The Nb5 group yielded an 83.3% hatching rate, higher body weight, lower gizzard ulceration and fewer FB1 residuals. In order to warrant the above results, 50 broilers aged 10 days were received orally with 20 ppm of FB1 for 20 days. At the same time, birds were fed orally with 50 μg of Nb5 or bivalent nanobody 11 (BiNb11). Finally, the Nb5 group showed a higher relative body weight gain and lower gastric ulcerations and fewer inflammations in the thymus and bursa. Conclusions: Based on the above evidence, the Nb5 nanobody may be considered as an additional FB1 detoxifier, contributing to FB1 decontamination.

Food-Borne Biotoxin Neutralization in Vivo by Nanobodies: Current Status and Prospects

“…82 The subsequent evaluation of its immunomodulatory potential showcased its significant cellular tolerance and resistance. 83 Additional endeavors, as undertaken by Tu et al 84 86 Their experiment involved administering 20 ppm of Fumonisin B1 orally to 50 ten-day-old broilers for a duration of 20 days. Concurrently, these birds were also orally fed with either 50 μg of Nb or bivalent Nb.…”

“…Advancements have also been made in developing Nbs against Fumonisin B1, which is a food-born mycotoxin produced by Fusarium moniliforme. Chen et al conducted meticulous animal experiments, specifically oral tests, which confirmed a substantial reduction in symptoms . Their experiment involved administering 20 ppm of Fumonisin B1 orally to 50 ten-day-old broilers for a duration of 20 days.…”

Avaliação da eficácia de diferentes adsorventes para redução de efeitos tóxicos combinados de micotoxinas na alimentação de vacas em lactação

“…A contaminação do leite bovino por ZEN é frequentemente observada (Falkauskas et al, 2022), assim como a presença de FUM (Y. Chen et al, 2022), e a co-ocorrência de diferentes micotoxinas (Frey et al, 2021).…”

“…Além disso, foi constatado que nanocorpos anti-FB 1 produzidos em Escherichia coli apresentaram uma remoção de 43,2% de FB 1 do leite quando utilizados na concentração de 1:2000 do nanocorpo 5 (Y. Chen et al, 2022). Na silagem de alfafa deteriorada, a fermentação promovida pela adição de LABs removeu 85,5% das micotoxinas (Zong et al, 2022).…”