A novel culture-independent method to search unknown dominant bacterial groups and its application to microbial analysis of membrane bioreactors

Abstract

13As we could not get numerical information for unknown unculturable microorganisms through 14 conventional culture-independent analysis methods such as next-generation sequencing, or real time 15 PCR, we developed an original culture-independent method, and searched the numerically dominant 16 bacteria in three industrial membrane bioreactors for livestock farms. 17