A novel culture medium for freshwater diatoms promotes efficient photoautotrophic batch production of biomass, fucoxanthin, and eicosapentaenoic acid

Gerin, Stéphanie; Delhez, Thomas; Corato, Amélie; Remacle, Claire; Franck, Fabrice

doi:10.1007/s10811-020-02097-1

Cited by 27 publications

(23 citation statements)

References 74 publications

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“…The dried lipids were resuspended in 1 mL methylation reactive [33% methanolic HCl 3N, 67% MeOH and 10 µg·mL −1 butylated hydroxytoluene (BHT)] in sealed vials, and transesterification was then performed at 70 °C, for 1 h. The FAMEs were then transferred in 800 µL of 100% heptane via four consecutive liquid–liquid extractions. They were quantified using gas chromatography (GC), and a flame ionization detector (FID, Shimadzu), as mentioned in [ 45 ]. The FAME separation was performed using SGE BPX70 column (30 m × 0.25 mm × 0.25 μm) with helium as the carrier gas.…”

Section: Methodsmentioning

confidence: 99%

A Fast-Growing Oleaginous Strain of Coelastrella Capable of Astaxanthin and Canthaxanthin Accumulation in Phototrophy and Heterotrophy

Corato

Baurain

et al. 2022

Life

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Considering the importance of microalgae as a promising feedstock for the production of both low- and high-value products, such as lipids and pigments, it is desirable to isolate strains which simultaneously accumulate these two types of products and grow in various conditions in order to widen their biotechnological applicability. A novel freshwater strain from the genus Coelastrella was isolated in Belgium. Compared to other Coelastrella species, the isolate presented rapid growth in phototrophy, dividing 3.5 times per day at a light intensity of 400 µmol·m−2·s−1 and 5% CO2. In addition, nitrogen depletion was associated with the accumulation of astaxanthin, canthaxanthin, and fatty acids, which reached ~30% of dry weight, and a majority of SFAs and MUFAs, which are good precursors for biodiesel. This strain also accumulated astaxanthin and canthaxanthin in heterotrophy. Although the content was very low in this latter condition, it is an interesting feature considering the biotechnological potential of the microalgal heterotrophic growth. Thus, due to its rapid growth in the light, its carotenogenesis, and its fatty acids characteristics, the newly identified Coelastrella strain could be considered as a potential candidate for biorefinery purposes of both low- and high-values products.

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Section: Methodsmentioning

confidence: 99%

A Fast-Growing Oleaginous Strain of Coelastrella Capable of Astaxanthin and Canthaxanthin Accumulation in Phototrophy and Heterotrophy

Corato

Baurain

et al. 2022

Life

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“…Fatty Acid Methyl Esters (FAMEs) were generated from the microalgal biomass as described in Corato et al (2022) . FAMEs quantification was performed using gas chromatography (GC, Shimadzu, Tokyo, Japan) and a flame ionization detector (FID, Shimadzu) as mentioned in Gérin et al (2020) . 1 μl of sample was injected on a SGE BPX70 column (30 m × 0.25 mm × 0.25 μm) in “split” mode with a ratio of 10.…”

Section: Methodsmentioning

confidence: 99%

Cell adaptation of the extremophilic red microalga Galdieria sulphuraria to the availability of carbon sources

et al. 2022

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were analyzed: heterotrophy with glycerol or glucose and phototrophy. This allowed us to understand the transcriptional response of cells to light and dark environments both at the nuclear and chloroplast levels, and to show that transcription of gene families, acquired by horizontal gene transfer, such as sugar, amino acid, or acetate transporters, were involved in the response to the availability of different (in)organic sources.

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“…It is the most widely used operational strategy for EPA production. Novel media development and optimization have been done to enhance the growth and EPA production of diatoms in phototrophic batch mode (Gérin et al, 2020). High biomass and EPA content of 5 g L −1 and 5% dry biomass, respectively, have been achieved in batch flat‐plate photobioreactors (Hulatt et al, 2017).…”

Section: Microalgae Cultivation For Epa Productionmentioning

confidence: 99%

Sustainable production of eicosapentaenoic acid-rich oil from microalgae: Towards an algal biorefinery

Sivakumar

Sachin

Priyadarshini

et al. 2022

Journal of Applied Microbiology

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Utilization of sustainable natural resources such as microalgae has been considered for the production of biofuels, aquaculture feed, high‐value bioactives such as omega‐3 fatty acids, carotenoids, etc. Eicosapentaenoic acid (EPA) is an omega‐3 fatty acid present in fish oil, which is of physiological importance to both humans and fish. Marine microalgae are sustainable sources of lipid rich in EPA, and different species have been explored for the production of EPA as a single product. There has been a rising interest in the concept of a multi‐product biorefinery, focusing on the maximum valorization of the algal biomass. Targeting one or more value‐added compounds in a biorefinery scenario can improve the commercial viability of low‐value products such as triglycerides for biofuel. This approach has been viewed by technologists and experts as a sustainable and economically feasible possibility for the large‐scale production of microalgae for its potential applications in biodiesel and jet fuel production, nutraceuticals, animal and aquaculture feeds, etc. In this review paper, we describe the recent developments in the production of high‐value EPA‐rich oil from microalgae, emphasizing the upstream and downstream bioprocess techniques, and the advantages of considering an EPA‐rich oil‐based biorefinery.

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A novel culture medium for freshwater diatoms promotes efficient photoautotrophic batch production of biomass, fucoxanthin, and eicosapentaenoic acid

Cited by 27 publications

References 74 publications

A Fast-Growing Oleaginous Strain of Coelastrella Capable of Astaxanthin and Canthaxanthin Accumulation in Phototrophy and Heterotrophy

A Fast-Growing Oleaginous Strain of Coelastrella Capable of Astaxanthin and Canthaxanthin Accumulation in Phototrophy and Heterotrophy

Cell adaptation of the extremophilic red microalga Galdieria sulphuraria to the availability of carbon sources

Sustainable production of eicosapentaenoic acid-rich oil from microalgae: Towards an algal biorefinery

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