A novel detergent additive: Organic solvent- and thermo-alkaline-stable recombinant subtilisin

Gülmez, Canan; Atakisi, Onur; Dalginli, Kezban Yıldız; Atakişi, Emine

doi:10.1016/j.ijbiomac.2017.11.133

Cited by 24 publications

(11 citation statements)

References 37 publications

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“…Catalysis in organic media with enzymes can provide important advantages in industrial applications. But, there are some difficulties due to enzymes that have low activity and instability in organic media [3]. The results showed that co-lyophilization of protease with pumice and KCl improved the catalytic activity in the presence of 2.5% and 5% of organic solvents when compared with lyophilized protease without additive (table 2, Enzyme).…”

Section: Organic Solvents Effect On Lyophilized Protease Preparationsmentioning

confidence: 97%

“…Furthermore, extracellular production of enzymes by microorganisms provides advantages like simple downstream processing and decreasing the costs [2]. The genus Bacillus is the most prominent source of protease with high yield [2][3][4]. Protease production from bacteria is generally performed with submerged fermentation (SmF) in shake flasks or fermenters [5].…”

Section: Introductionmentioning

confidence: 99%

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a PRODUCTION OF PROTEASE FROM BACILLUS SUBTILIS UNDER SSF AND EFFECT OF ORGANIC SOLVENTS ON LYOPHILIZED PROTEASE PREPARATIONS

2020

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Objective: In the present work, protease was produced from Bacillus subtilis under solid-state fermentation (SSF). The effect of lyophilization with different additives on the activity of protease in an organic solvent and kineteci properties was investigated. Methods: Production conditions of protease (fermentation time, moisture level, initial pH, temperature) were optimized. After production, it was partially purified and then, lyophilized with different additives from an aqueous buffer solution containing 98% (w/w) of different additives (pumice, KCl, without additive) for 72 h after freezing in liquid nitrogen. After that, the effect of organic solvents (2.5% and 5% of DCM, ethanol, hexane, toluene) on these lyophilized protease preparations was determined and their kinetic properties were determined. Results: Optimum protease production was obtained with 40% of moisture level, at pH 7.5, 37 °C after 24 h fermentation. It was partially purified by using ammonium sulphate precipitation (20-80%) with 5.8-fold and specific activity of 38 U/mg and then dialysed with 6.4-fold and a specific activity of 35 U/mg. Co-lyophilization of protease with pumice and KCl was increased activity of an enzyme in aqueous organic solvents when compared lyophilized protease without additive. Used solvents, except DCM, were increased activity of lyophilized protease with pumice/KCl. It was found that the lyophilization with pumice and KCl resulted in an increasing in the catalytic efficiency, while it was decreased in Km and Vmax values. Conclusion: The obtained findings demonstrated that protease from B. subtilis can effectively be produced under SSF by using wheat bran and used in industrial applications because of showing improved activity in an organic solvent by co-lyophilization with pumice/KCl.

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Section: Organic Solvents Effect On Lyophilized Protease Preparationsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

a PRODUCTION OF PROTEASE FROM BACILLUS SUBTILIS UNDER SSF AND EFFECT OF ORGANIC SOLVENTS ON LYOPHILIZED PROTEASE PREPARATIONS

2020

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“…The production and application of enzymes has been growing every year, not only in biochemical research, but also in the chemical industry, food industry and pharmaceutical industry . Under appropriate conditions of application, enzymes have specificity for substrates, accelerating reaction rates and thus actively participating in industrial manufacture of added value products .…”

Section: Introductionmentioning

confidence: 99%

“…P45, is characterized by catalyzing the hydrolysis of a variety of substrates, including rigid structures containing keratin . In this context, the characterization of proteases is extremely important for improving the understanding on the catalytic behavior and predicting the outcome when the enzyme is used in an industrial process …”

Section: Introductionmentioning

confidence: 99%

“…11,12 In this context, the characterization of proteases is extremely important for improving the understanding on the catalytic behavior and predicting the outcome when the enzyme is used in an industrial process. 2,13 The effect organic solvents on the activity and stability differs according to the origin of the protease and the character of solvent. 14 Several proteases have shown stability and/ or increased activity in aqueous-hydrophobic organic mixtures, 14,15 suggesting the ability of these enzymes to form multiple hydrogen bonds with water to maintain the structural flexibility for optimal catalysis.…”

Section: Introductionmentioning

confidence: 99%

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Thermal stability and catalytic properties of protease from Bacillus sp. P45 active in organic solvents and ionic liquid

Borba

Machado

Brandelli

et al. 2018

Biotechnology Progress

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The parameters half-life, z value, enthalpy, entropy, and free energy were evaluated in the temperature range of 40 to 55°C for a Bacillus sp. P45 protease present in a medium composed of ionic liquid (IL) and organic solvent. The protease was previously treated in IL [Emim][Tf N] and increased activity was observed in four out of five organic solvents tested. The reaction medium containing acetone and IL (1:1 v/v ratio) was more stable than the buffer medium, with half-life of 2.4 h at 55°C. Thermodynamic parameters values showed there was probably less rupture of non-covalent bonds that stabilize the protein structure, which promoted the protease P45 stabilization. According to this study it was concluded that the protease P45 in a medium composed of [Emim][Tf N] and acetone may be used at higher temperatures than those employed in buffer or medium containing organic solvent. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 2018 © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1102-1108, 2018.

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Co‐production of Biosurfactants and Other Bioproducts in Biorefineries

Vélez‐Mercado,

Espinosa‐Lavenant,

Flores‐Iga

et al. 2023

Biosurfactants and Sustainability

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A novel detergent additive: Organic solvent- and thermo-alkaline-stable recombinant subtilisin

Cited by 24 publications

References 37 publications

a PRODUCTION OF PROTEASE FROM BACILLUS SUBTILIS UNDER SSF AND EFFECT OF ORGANIC SOLVENTS ON LYOPHILIZED PROTEASE PREPARATIONS

a PRODUCTION OF PROTEASE FROM BACILLUS SUBTILIS UNDER SSF AND EFFECT OF ORGANIC SOLVENTS ON LYOPHILIZED PROTEASE PREPARATIONS

Thermal stability and catalytic properties of protease from Bacillus sp. P45 active in organic solvents and ionic liquid

Co‐production of Biosurfactants and Other Bioproducts in Biorefineries

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