A Novel Diagnostic Method to Detect Duck Tembusu Virus: A Colloidal Gold-Based Immunochromatographic Assay

Yu, Guanliu; Yu, Xianglong; Yang, Guoping; Tang, Yi; Diao, Youxiang

doi:10.3389/fmicb.2018.01001

Cited by 12 publications

(9 citation statements)

References 49 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DTMUV is a major pathogen that endangers waterfowl, and has caused several billion dollars of losses in the Chinese poultry industry since 2010 [ 16 ]. Increasing our knowledge of the genetic evolution of DTMUV is important for realizing the virus protein mutations, and gaining an insight into the protein structure can also provide strategies for vaccine development, which further lay a foundation for controlling the prevalence of DTMUV infection.…”

Section: Discussionmentioning

confidence: 99%

Evolution of Tembusu Virus in Ducks, Chickens, Geese, Sparrows, and Mosquitoes in Northern China

Lin

Tang

et al. 2018

Viruses

Self Cite

View full text Add to dashboard Cite

Tembusu virus (TMUV) is a contagious pathogen from fowl that mainly infects ducks and geese, causing symptoms of high fever, loss of appetite, retarded growth, neurological symptoms, severe duck-drop syndrome, and even death. During an epidemiological investigation of TMUV in Northern China, we isolated 11 TMUV strains from ducks, chickens, geese, sparrows, and mosquitoes (2011–2017). Phylogenetic analysis of the open-reading frames of genes revealed that these strains clustered into Chinese strains II. The nucleotide and amino acid homologies of NS1 of the strains ranged between 85.8–99.8% and 92.5–99.68%, respectively, which were lower than those of E (86.7–99.9% and 96.5–99.9%, respectively), NS3 (87.6–99.9% and 98.2–99.8%, respectively), and NS5 (86.5–99.9% and 97.8–99.9%, respectively). Predictions of the tertiary structure of the viral proteins indicated that NS1 in 4 of 11 strains had a protein structure mutation at 180TAV182 that changed a random crimp into an alpha helix. The protein of 6 of 11 strains had a glycosylation site mutation from NTTD to NITD. Furthermore, epidemiological data suggested that TMUV has been circulating in half of China’s provinces (17 of 34). Our findings, for the first time, have identified the NS1 protein as a potential hypervariable region for genetic evolution. Additionally, the territorial scope of the virus has expanded, requiring strict bio-security measures or a multivalent vaccine to control its spread.

show abstract

Section: Discussionmentioning

confidence: 99%

Evolution of Tembusu Virus in Ducks, Chickens, Geese, Sparrows, and Mosquitoes in Northern China

Lin

Tang

et al. 2018

Viruses

Self Cite

View full text Add to dashboard Cite

show abstract

“…An immunochromatography assay (ICA) determines the presence or absence of a target analyte, such as pathogens or biomarkers [ 16 , 17 ]. A schematic diagram of ICA is shown in Fig.…”

Section: Introductionmentioning

confidence: 99%

Broad-range and effective detection of human noroviruses by colloidal gold immunochromatographic assay based on the shell domain of the major capsid protein

Lyu

et al. 2021

BMC Microbiol

View full text Add to dashboard Cite

Background Human noroviruses (HuNoVs) are a major cause of nonbacterial gastroenteritis in all age groups worldwide. HuNoVs can be detected in vitro using molecular assays such as RT-PCR and RT-qPCR. However, these molecular-based techniques require special equipment, unique reagents, experienced personnel, and extended time to obtain results. Besides, the diversity of viral genotypes is high. Therefore, methods that are rapid, broad-range and effective in the detection of HuNoVs are desiderated for screening the feces or vomit of infected people during outbreaks. Results In this study, a colloidal-gold-based immunochromatographic assay (ICA) was developed for effective detection of HuNoVs in clinical samples. Monoclonal antibodies (MAbs) against the shell (S) domain in the major capsid protein of HuNoVs were used in the ICA. The limitations of detection for HuNoVs in clinical samples were 1.2 × 106 genomic copies per gram of stool sample (gc/g) and 4.4 × 105 gc/g for genogroup I and II (GI and GII) HuNoVs, respectively. A total of 122 clinical samples were tested for HuNoVs by ICA and compared against RT-qPCR. The relative sensitivity, specificity and agreement of ICA was 84.2% (95% CI: 83.6–84.8%), 100.0% (95% CI: 98.5–100.0%) and 87.7% (95% CI: 85.6–89.8%), respectively. No cross-reaction with other common enteric viruses or bacteria was observed. The ICA detected a broad range of genotypes, including GI.1, GI.3, GI.4, GI.6, GI.14, GII.2, GII.3, GII.4, GII.6, GII.13, and GII.17 HuNoVs. Conclusions This study demonstrates that ICA targeting the S domain of VP1 is a promising candidate for effectively identifying the different genotypes of HuNoVs in clinical samples with high sensitivity and specificity.

show abstract

“…The disease is characterized by a substantial drop in egg-laying and low mortality [1]. The causative agent has been identified as duck Tembusu virus (DTMUV), an enveloped positive sense single-stranded RNA virus that belongs to the genus Flavivirus, family Flaviviridae [2][3][4]. DTMUV, similar to other flaviviruses, is a mosquito-borne Flavivirus, and was first isolated from mosquitoes of the genus Culex in 1970s in Malaysia [5].…”

Section: Introductionmentioning

confidence: 99%

pUC18-CpG Is an Effective Adjuvant for a Duck Tembusu Virus Inactivated Vaccine

Ren

Wang

Shan

et al. 2020

Viruses

View full text Add to dashboard Cite

Duck Tembusu virus (DTMUV) is an emerging pathogenic flavivirus responsible for massive economic losses in the duck industry. However, commercially inactivated DTMUV vaccines have been ineffective at inducing protective immunity in ducks. The widely used adjuvant cytosine-phosphate-guanine oligodeoxynucleotides (CpG ODNs) reportedly improve humoral and cellular immunities in animal models. However, its effectiveness in DTMUV vaccines requires validation. Here, we assessed the protective efficacy of pUC18-CpG as an adjuvant in an inactivated live DTMUV vaccine in ducks. Our results revealed that the serum hemagglutination inhibition (HI) antibody titers, positive rates of anti-DTMUV antibodies, the concentration of serum cytokines, and protection efficacy were significantly increased in ducks immunized with pUC18-CpG compared to that in the control group. Moreover, ducks immunized with a full vaccine dose containing a half dose of antigen supplemented with 40 µg of pUC18-CpG exhibited the most potent responses. This study suggests that pUC18-CpG is a promising adjuvant against DTMUV, which might prove effective in treating other viral diseases in waterfowl.Viruses 2020, 12, 238 2 of 11 can also infect humans [14]. Therefore, DTMUV can pose a severe economic burden on the poultry industry while simultaneously constituting a public health threat.Although an effective attenuated DTMUV vaccine candidate has been reported [15], immunization with this type of vaccine hampers the differentiation of infected and vaccinated ducks based on serology. In addition, the cost of attenuated vaccines is prohibitive, and improper administration may cause potential virulence reversion [16]. Alternatively, an inactivated live DTMUV vaccine is available; however, it has been shown to induce low protective antibody titers in ducks and, thus, is not completely effective in protecting immunized ducks [17]. A more cost-effective and safer DTMUV vaccine variant is, therefore, urgently needed to tackle this emergent disease and assist the duck industry.The bacterial genomic DNA, or synthetic unmethylated cytosine-phosphate-guanine oligodeoxynucleotides (CpG ODNs), which contain bioactive CpG motifs, elicit immunostimulatory effects and are considered safe and effective immunopotentiator adjuvants. These molecules reportedly enhance humoral and cellular immunity in humans and animals [18][19][20]. It has now been recognized that the human immune system, similar to other vertebrates, identifies unmethylated CpG dinucleotides in specific situations involving dangerous stimuli or infection, thereby activating immune response cascades [21]. Moreover, studies have shown that when the exogenous CpG motif is inserted into an empty plasmid vector (pUC-18), it significantly enhances the immune response to a vaccine [22,23]. To the best of our knowledge, CpG ODNs have preliminarily been explored as immunostimulants or adjuvants in waterfowl (including ducks), and satisfactory results have been achieved [24,25].This study evaluated the adjuvant potenti...

show abstract

A Novel Diagnostic Method to Detect Duck Tembusu Virus: A Colloidal Gold-Based Immunochromatographic Assay

Cited by 12 publications

References 49 publications

Evolution of Tembusu Virus in Ducks, Chickens, Geese, Sparrows, and Mosquitoes in Northern China

Evolution of Tembusu Virus in Ducks, Chickens, Geese, Sparrows, and Mosquitoes in Northern China

Broad-range and effective detection of human noroviruses by colloidal gold immunochromatographic assay based on the shell domain of the major capsid protein

pUC18-CpG Is an Effective Adjuvant for a Duck Tembusu Virus Inactivated Vaccine

Contact Info

Product

Resources

About