A novel dielectrophoresis-based device for the selective retention of viable cells in cell culture media

Docoslis, Aristides; Kalogerakis, Nicolas; Behie, Leo A.; Kaler, K.V.I.S.

doi:10.1002/(sici)1097-0290(19970505)54:3<239::aid-bit5>3.0.co;2-g

Cited by 55 publications

(32 citation statements)

References 45 publications

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“…While the immune system of organisms can identify and remove dead and dying cells from the body, the presence of debris and dead cells in in vitro cell culture adversely affects its quality and productivity. Enrichment for viable cells can also improve the accuracy of biomedical assays, as quantification can be made inaccurate by the presence of nonviable cells in sample preparation or lead to improper conclusions in monitoring the effect of experimental compounds 9–12 .…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Sorting of Cells by Viability Based on Differences in Cell Stiffness

Islam

Brink

Blanche

et al. 2017

Sci Rep

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The enrichment of viable cells is an essential step to obtain effective products for cell therapy. While procedures exist to characterize the viability of cells, most methods to exclude nonviable cells require the use of density gradient centrifugation or antibody-based cell sorting with molecular labels of cell viability. We report a label-free microfluidic technique to separate live and dead cells that exploits differences in cellular stiffness. The device uses a channel with repeated ridges that are diagonal with respect to the direction of cell flow. Stiff nonviable cells directed through the channel are compressed and translated orthogonally to the channel length, while soft live cells follow hydrodynamic flow. As a proof of concept, Jurkat cells are enriched to high purity of viable cells by a factor of 185-fold. Cell stiffness was validated as a sorting parameter as nonviable cells were substantially stiffer than live cells. To highlight the utility for hematopoietic stem cell transplantation, frozen samples of cord blood were thawed and the purity of viable nucleated cells was increased from 65% to over 94% with a recovery of 73% of the viable cells. Thus, the microfluidic stiffness sorting can simply and efficiently obtain highly pure populations of viable cells.

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Section: Introductionmentioning

confidence: 99%

Microfluidic Sorting of Cells by Viability Based on Differences in Cell Stiffness

Islam

Brink

Blanche

et al. 2017

Sci Rep

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“…The use of nonuniform electric fields for the manipulation of mm-sized objects is well documented [28][29][30][31][32][33][34][35][36]. A variety of applications including collection, fusion, and separation of biological cells [37], immobilization of DNA [38], collection of viral particles [39], and alignment of nanospheres [40], have been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

One-, two-, and three-dimensional organization of colloidal particles using nonuniform alternating current electric fields

Docoslis

Alexandridis

2002

Electrophoresis

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We demonstrate here the use of nonuniform alternating current (AC) electric fields, generated by planar electrodes, for the organization of num-sized particles into one-, two-, and three-dimensional assemblies. The electrodes, with separations that vary from 35 to 300 num, are made of gold deposited on glass substrata. Latex, silica and graphite particles have been examined inside organic or aqueous media in order to illustrate the general applicability of the technique. Theoretical predictions of the particle response under the electric fields are experimentally confirmed for all the above particle/media combinations and can thus be used as a valuable design tool. The size and shape of the final structures are mainly dependent on the electrode shape and dimensions, but are also subject to the particle type and operating conditions. Particle organization in one dimension (strings) is achieved under conditions of positive or negative dielectrophoresis in the space between two energized electrodes. Two-dimensional particle organization (ordered, planar particles assemblies) was observed under conditions of negative dielectrophoresis, when quadrupole electrodes were employed. Moreover, when negative dielectrophoresis and stronger electric fields are applied (of the order of 50 kV(rms) m(-1)), three-dimensional, pyramid-like structures with a vertical dimension 1000-fold higher than that of the corresponding (planar) electrodes can be assembled. These 3-D structures can grow as free-standing assemblies, or inside templates etched in the substratum. The dielectrophoresis (DEP)-organized particle assemblies can subsequently be rendered permanent via the in situ fixing (cross-linking) of the individual particles.

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“…This has the advantage of allowing the use of two separate outlets to be used, one for each of the two populations to be separated. Flow separator systems have been demonstrated to be effective for the separation of cancer cells [13,35,36,38,[41][42][43][44], cells in culture [45] and yeast cells [12,46], but have increasingly been superseded by field-flow fractionation devices for separation of many populations, rather than the straightforward binary separation of flow methods. One possible future application may be as a dielectrophoretic barrier to particles entering the device, as recently demonstrated by Dussaud et al [47].…”

Section: Flow Separationmentioning

confidence: 99%

Strategies for dielectrophoretic separation in laboratory-on-a-chip systems

2002

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Dielectrophoresis -the induced motion of polarizable particles in nonuniform fieldshas proven to be an effective method for the separation of bioparticles such as cells, viruses and proteins. In this paper, the application of dielectrophoresis in miniaturized laboratories-on-a-chip is discussed, and strategies are described for using dielectrophoresis both for the binary separation of bioparticles and for the fractionation of many populations in such devices.

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A novel dielectrophoresis-based device for the selective retention of viable cells in cell culture media

Cited by 55 publications

References 45 publications

Microfluidic Sorting of Cells by Viability Based on Differences in Cell Stiffness

Microfluidic Sorting of Cells by Viability Based on Differences in Cell Stiffness

One-, two-, and three-dimensional organization of colloidal particles using nonuniform alternating current electric fields

Strategies for dielectrophoretic separation in laboratory-on-a-chip systems

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